Back to Journals » International Journal of Nanomedicine » Volume 7

Mannosylated liposomes for targeted gene delivery

Authors Kong F, Zhou, Ge, Liu, Wang

Received 14 December 2011

Accepted for publication 12 January 2012

Published 22 February 2012 Volume 2012:7 Pages 1079—1089

DOI https://doi.org/10.2147/IJN.S29183

Review by Single-blind

Peer reviewer comments 5


Fansheng Kong1, Fang Zhou1, Linfu Ge1, Ximin Liu1, Yong Wang2
1Department of Hematology, 2Department of Rehabilitation and Physiotherapy, General Hospital of Ji'nan Command, PLA, Ji'nan, People's Republic of China

Background: Liposomes can be modified with different ligands to control their biological properties, such as longevity, targeting ability, and intracellular penetration, in a desired fashion. The aim of this study was to modify liposomes with a novel mannosylated polyethylene glycol-phosphatidylethanolamine (M-PEG-PE) ligand to achieve active targeted gene delivery.
Methods: Rat Kupffer cells were isolated and used as model cells for in vitro evaluation of cytotoxicity and transfection efficiency. The modified liposomes were intravenously injected into the rats, and Kupffer cells were isolated and analyzed by flow cytometry for in vivo gene delivery and expression.
Results: The M-PEG-PE-modified liposome-enhanced green fluorescence protein plasmid (M-PEG-PE-Lipo-pEGFP) complexes had a particle size of 237 nm and a loading efficiency of 90%. The M-PEG-PE-Lipo-pEGFP complexes displayed remarkably higher transfection efficiency than unmodified Lipo-pEGFP, both in vitro (51%–30%) and in vivo (43%–27%).
Conclusion: M-PEG-PE could function as an excellent active targeting ligand, and M-PEG-PE-modified liposomes could be promising active targeted drug delivery vectors.

Keywords: gene delivery, active targeting, mannosylated, polyethylene glycol, phosphatidylethanolamine, liposomes

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]