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Long-term complete remission in a patient with intravascular large B-cell lymphoma with central nervous system involvement

Authors Sawada T, Omuro Y, Kobayashi T, Hishima T, Koizumi F, Kanemasa Y, Shimoyama T, Sasaki E, Maeda Y

Received 12 August 2014

Accepted for publication 4 October 2014

Published 18 November 2014 Volume 2014:7 Pages 2133—2136

DOI https://doi.org/10.2147/OTT.S72596

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 4

Editor who approved publication: Dr Faris Farassati



Takeshi Sawada,1 Yasushi Omuro,1 Takeshi Kobayashi,2 Tunekazu Hishima,3 Fumiaki Koizumi,4 Yusuke Kanemasa,1 Tatsu Shimoyama,1 Eisaku Sasaki,1 Yoshiharu Maeda1

1Department of Chemotherapy, 2Department of Hematology, 3Department of Pathology, 4Department of Laboratory Medicine, Tokyo Metropolitan Cancer and Infectious Diseases Center, Komagome Hospital, Bunkyo-ku, Tokyo, Japan


Abstract: This report describes a patient with intravascular large B-cell lymphoma (IVLBCL) with central nervous system involvement at the time of diagnosis who achieved complete remission for over 5 years in response to therapy. The patient, a 71 year-old woman, was previously healthy with the exception of taking verapamil for paroxysmal supraventricular tachycardia. She had presented with pyrexia and gradually progressive anemia. Brain magnetic resonance imaging revealed an infarct-like lesion in the pons, although no paralysis was observed. She was diagnosed with IVLBCL on the basis of random skin biopsy. After eight cycles of rituximab plus cyclophosphamide, doxorubicin, vincristine, and prednisone therapy, abnormal laboratory data had normalized, and no pontine lesion was evident on magnetic resonance imaging without receiving any intrathecal chemotherapy. IVLBCL is associated with poor prognosis, particularly in patients with central nervous system involvement. Early initiation of rituximab plus cyclophosphamide, doxorubicin, vincristine, and prednisone therapy and drug interactions between anticancer agents and verapamil as a p-glycoprotein inhibitor were considered the possible reasons for favorable outcome in the present case.

Keywords: intravascular large B-cell lymphoma, random skin biopsy, CNS involvement, rituximab, verapamil, blood–brain barrier

Introduction

Intravascular large B-cell lymphoma (IVLBCL) is a rare and aggressive variant of extranodal large B-cell lymphoma with frequent involvement of the central nervous system (CNS) characterized by proliferation of large lymphoma cells within the lumina of the small vessels.1 The lack of characteristic clinical symptoms and the absence of lymphadenopathy can make the diagnosis of IVLBCL quite difficult, and the prognosis for patients with this disease is extremely poor.2 The recent use of random skin biopsies has enabled earlier diagnosis of IVLBCL.3 While the anti-CD20 monoclonal antibody, rituximab, can improve survival for patients with IVLBCL,4 those with CNS involvement still have poor outcomes.5 The present report describes the first reported case of a patient with IVLBCL who was diagnosed in the early phase by a random skin biopsy and who was successfully treated with rituximab plus cyclophosphamide, doxorubicin, vincristine, and prednisone (R-CHOP) therapy without any intrathecal treatment.

Case presentation

A 71 year-old woman had intermittent pyrexia (>38°C) for 1 week in May 2009. Her family doctor treated her with a course of antibiotics to no effect. Computed tomography (CT), cardiac ultrasonography, and tuberculosis skin test detected no abnormalities. General malaise gradually developed, and hemoglobin (Hb) level fell from 10.2 g/dL to 9.0 g/dL over 2 weeks. She was admitted to our hospital as a case of fever of unknown origin. On admission, pyrexia was the only abnormality on vital signs. She was taking no medications other than verapamil and digoxin for paroxysmal supraventricular tachycardia. Meticulous physical examination detected anemic conjunctivae and edema of the bilateral lower extremities. No skin lesions, palpable lymph nodes, or neurological abnormalities were evident. Laboratory findings showed anemia (Hb, 8.8 g/dL) and elevated levels of lactate dehydrogenase (454 IU/L) and soluble interleukin-2 receptor (sIL2-R, 6,030 U/mL). Commonly used autoantibodies and tumor markers for assessment of collagen diseases and cancers were all negative. CT scan revealed no lymphadenopathy, hepatosplenomegaly, or abnormal lung lesions. Gallium scintigraphy showed no abnormal accumulations. Brain magnetic resonance imaging (MRI) revealed a non-enhancing, high-intensity area of 17 mm in diameter in the pons on T2- and diffusion-weighted imaging (Figure 1A and B); however, no abnormal neurological signs were observed. Cerebrospinal fluid examination likewise found no abnormalities. Based on the high sIL2-R level, IVLBCL was suspected. A bone marrow biopsy showed a normocellular marrow with no apparent lymphoma cells. Genetic analysis of the bone marrow specimen showed no clonal rearrangement of T-cell receptor Cβ1 or the immunoglobulin heavy chain JH region gene. We took healthy-appearing skin randomly from the forearm, lower abdomen, and thigh for biopsy although no skin lesions were observed. All specimens revealed large B lymphoma cells within small veins and capillaries of the subcutaneous fat tissues but not outside the vessels (Figure 2A and B). Immunohistochemical studies showed that lymphoma cells were positive for CD20 (Figure 3), a B-cell marker, and negative for CD3, a T-cell marker. Based on these findings, a diagnosis of IVLBCL was made. The patient’s poor general condition required a less toxic regimen, thus conventional R-CHOP therapy, consisting of rituximab (375 mg/m2), cyclophosphamide (750 mg/m2), doxorubicin (50 mg/m2), vincristine (1.4 mg/m2), and prednisolone (60 mg/m2), was started within 1 month of initial symptoms. After the first lumbar puncture for spinal fluid examination, the patient did not accept repeating it due to its invasiveness. For this reason, we treated the patient without any intrathecal treatment. After the first cycle of systemic chemotherapy, the patient had resolution of fever and peripheral edema, as well as improvement in Hb, lactate dehydrogenase, and sIL2-R levels. After eight cycles of R-CHOP therapy, the pontine lesion had completely disappeared (Figure 4A and B). Therefore, the asymptomatic reversible pontine lesion was considered as IVLBCL involvement. During follow-up, the value of sIL2-R had been within reference range. Currently at 5 years after diagnosis, the patient has been doing well with no recurrence and returned to work without any neurological disorders.

Figure 1 The image shows a 17 mm high intensity area in the pons on T2-weighted (A) and diffusion-weighted (B) magnetic resonance imaging (MRI) of the brain.

Figure 2 Pathological specimens.
Notes: (A) Skin biopsy specimen taken from the lower abdomen reveals proliferation of large lymphoma cells filling the vessels of hypodermic adipose tissue. (B) Numerous lymphoma cells with irregular nuclear contours and large nucleoli clustered are seen in small vessel lumens. Hematoxylin and eosin staining; magnification (A) ×40 (B) ×100.

Figure 3 Immunohistochemistry demonstrating positivity of intravascular lymphoma cells for CD20.
Note: Magnification ×100.

Figure 4 Magnetic resonance imaging (MRI) of the brain after receiving chemotherapy.
Notes: Pontine lesion on T2-weighted (A) and diffusion-weighted (B) MRI of the brain completely resolved after eight cycles of rituximab plus cyclophosphamide, doxorubicin, vincristine, and prednisone therapy.

Discussion

The present report describe a case of IVLBCL with CNS involvement that achieved complete remission for at least 5 years in response to R-CHOP therapy. Patients with IVLBCL with CNS involvement have a high risk of developing CNS recurrence and poor outcomes, even if they receive intensive chemotherapy like autologous stem cell transplantation.5 There is no reported case that had maintained complete remission for over 5 years.

Clinical manifestations of IVLBCL are known to differ between Asian and Western countries.4 In Western countries, IVLBCL often involves the skin and CNS. While IVLBCL in Asian countries tends to show only few skin lesions,6 random skin biopsy still appears useful to diagnose IVLBCL in Japan.3,7 In the present case, biopsied specimens were taken from apparently healthy skin. Even when biopsy is not easily available, in a case where IVLBCL is clinically suspected, for example, when the patient has an increased level of sIL2-R with or without lymphadenopathy, it is advisable to include random skin biopsies in the initial work-up to facilitate early diagnosis.

Rituximab-containing chemotherapies appear to improve clinical outcomes for IVLBCL patients.4 Additionally, a retrospective analysis demonstrated that rituximab might reduce the risk of CNS recurrence in IVLBCL patients.5 Those facts indicate that IVLBCL should be treated with regimens including rituximab. Although a few case reports showed that a patient with IVLBCL with pontine involvement was successfully treated with a rituximab containing regimen,8,9 the treatment effect of rituximab against IVLBCL with CNS involvement has not been clarified.4,5 Previous reports proposed that patients with IVLBCL with CNS involvement should be treated with regimens similar to those used for primary or secondary CNS lymphoma,10 including agents showing higher bioavailability in the CNS such as methotrexate or cytarabine.11,12 Besides, a case report suggested that intrathecal chemotherapy is a useful treatment for IVLBCL with CNS involvement.8 However, the results in this case suggest that R-CHOP can be effective for early IVLBCL with CNS lesions. In fact, the blood–brain barrier (BBB) is likely to block access of drugs to sites of infiltrative tumors; thus, the effectiveness of R-CHOP on CNS lymphomas is not promising.13 In that respect, as lymphoma cells must be present in vessels, drug efficacy for IVLBCL does not necessarily reflect vascular permeability; however, some IVLBCL cases indicate that minimal extravascular infiltration around the involved vessels occurs in the course of the disease.14 Therefore, early diagnosis and early initiation of R-CHOP may have contributed to the favorable response, despite CNS involvement, in the present case.

Regarding the drug metabolism, verapamil and all drugs contained in CHOP regimen are metabolized by liver enzyme cytochrome P 3A4 (CYP3A4). Due to this fact, it is worth considering that the metabolization of these drugs was affected by the drug–drug interaction, although it is difficult to analyze plasma drug concentrations retrospectively.

In the present case, the patient was using 80 mg/day of verapamil and 0.25 mg/day of digoxin for paroxysmal supraventricular tachycardia. Verapamil is a potent inhibitor of p-glycoprotein, which plays an important role in anticancer drug resistance in malignant lymphoma15 and limits the access of systemic drugs across the BBB.16 Digoxin is also a substrate of p-glycoprotein and might modulate BBB permeability to various drugs.17 In in vitro experiments in a previous report, inhibition of p-glycoprotein using 0.1 μM/L of verapamil resulted in reversal of p-glycoprotein-mediated doxorubicin and vincristine resistance in lymphoma cells.18 According to the manufacturer’s data sheet of verapamil, the Cmax (the maximum serum concentration of drug) of 80 mg of verapamil sufficiently reaches 0.1 μM/L. In fact, a combinational treatment effect between verapamil and anticancer agents for lymphoma patients has been reported previously.15 Therefore, taking verapamil during chemotherapy might contribute to complete remission despite CNS involvement in the present case.

In conclusion, treatment strategies for IVLBCL with CNS involvement are still controversial. The patient showed a favorable outcome after early diagnosis and the chemotherapy with verapamil. The present case suggests that early initiation of therapy and chemotherapy combined with p-glycoprotein inhibitors play an important role in treatment of IVLBCL with CNS involvement. Further retrospective investigation into the time of initiation of therapy and drug interaction between p-glycoprotein inhibitors and anticancer agents in IVLBCL patients is needed.

Disclosure

The authors report no conflicts of interest in this work.


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