Infectious complications in children with malignant bone tumors: a multicenter nationwide study
Authors Czyzewski K, Galazka P, Zalas-Wiecek P, Gryniewicz-Kwiatkowska O, Gietka A, Semczuk K, Chelmecka-Wiktorczyk L, Zak I, Salamonowicz M, Fraczkiewicz J, Zajac-Spychala O, Bien E, Plonowski M, Wawrykow P, Pierlejewski F, Gamrot Z, Malas Z, Stolpa W, Musial J, Styczynski J
Received 27 December 2018
Accepted for publication 29 March 2019
Published 30 May 2019 Volume 2019:12 Pages 1471—1480
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 3
Editor who approved publication: Dr Joachim Wink
Krzysztof Czyzewski,1 Przemyslaw Galazka,2 Patrycja Zalas-Wiecek,3 Olga Gryniewicz-Kwiatkowska,4 Agnieszka Gietka,4 Katarzyna Semczuk,5 Liliana Chelmecka-Wiktorczyk,6 Iwona Zak,7 Malgorzata Salamonowicz,8 Jowita Fraczkiewicz,8 Olga Zajac-Spychala,9 Ewa Bien,10 Marcin Plonowski,11 Pawel Wawrykow,12 Filip Pierlejewski,13 Zuzanna Gamrot,14 Zofia Malas,15 Weronika Stolpa,16 Jakub Musial,17 Jan Styczynski1
1Department of Pediatric Hematology and Oncology, Collegium Medicum, Nicolaus Copernicus University Torun, Bydgoszcz, Poland; 2Department of General and Oncological Surgery for Children and Adolescents, Collegium Medicum, Nicolaus Copernicus University Torun, Bydgoszcz, Poland; 3Department of Microbiology, Collegium Medicum, Nicolaus Copernicus University Torun, Bydgoszcz, Poland; 4Department of Oncology, Children’s Memorial Health Institute, Warsaw, Poland; 5Department of Microbiology, Children’s Memorial Health Institute, Warsaw, Poland; 6Department of Pediatric Oncology and Hematology, University Children’s Hospital, Jagiellonian University, Collegium Medicum, Krakow, Poland; 7Department of Microbiology, University Children’s Hospital, Jagiellonian University, Collegium Medicum, Krakow, Poland; 8Department of Pediatric Stem Cell Transplantation, Hematology and Oncology, Medical University, Wroclaw, Poland; 9Department of Pediatric Oncology, Hematology and Transplantology, University of Medical Sciences, Poznan, Poland; 10Department of Pediatrics, Hematology and Oncology, Medical University, Gdansk, Poland; 11Department of Pediatric Oncology and Hematology, Medical University, Bialystok, Poland; 12Department of Pediatrics and Pediatric Oncology, Pomeranian Medical University, Szczecin, Poland; 13Department of Pediatric Oncology and Hematology, Medical University, Lodz, Poland; 14Division of Pediatric Hematology and Oncology, Chorzow City Hospital, Chorzow, Poland; 15Division of Pediatric Hematology and Oncology, Children Hospital, Olsztyn, Poland; 16Division of Pediatric Oncology, Hematology and Chemotherapy, Department of Pediatric, Silesian Medical University, Katowice, Poland; 17Department of Pediatric Oncohematology, Medical Faculty University of Rzeszow, Clinical Provincial Hospital No. 2, Rzeszow, Poland
Objectives: The analysis of epidemiology, risk factors and outcome of infections in children with malignant bone tumors (MBT) undergoing chemotherapy.
Methods: In this retrospective nationwide multicenter cross-sectional study, a total number of 126 children with MBT including 70 with Ewing sarcoma (ES) and 56 with osteosarcoma (OSA) were screened for infections over a period of 72 consecutive months.
Results: The risk of infection was 7.15-fold higher in patients with ES as compared to the OSA group, especially concerning bacterial infections (4.1-fold increase risk). Bacterial infections occurred in 74.3% patients with ES and in 41.1% with OSA. The median time from diagnosis to first infection was 4.9 months. 33.0% of bacterial episodes were diagnosed as bloodstream (BSI), 31.1% as gastrointestinal tract, 30.1% as urinary tract infection. Infection-related mortality (IRM) from bacterial infection was 6% and 15% in ES and OSA patients, respectively. Cumulative incidence was 7.1% for invasive fungal disease and 6.3% for viral infections. The only significant risk factor for IRM was time to infection ≥5 months since the beginning of chemotherapy. All patients who have died from infection had BSI and were in neutropenia.
Conclusions: Infections in the children with MBT in our study occurred with high frequency, especially in patients with ES. The most frequent were bacterial infections, while fungal and viral infections were episodic. Among the bacterial infections, bloodstream, urinary tract and gastrointestinal tract infections occurred with similar frequency. All deceased patients died due to BSI. Bacterial infection occurring ≥5 months since the beginning of chemotherapy was a risk factor for death.
Keywords: infectious complications, risk factors analysis, Ewing sarcoma, osteosarcoma, malignant bone tumor, children
Infections are the major cause of morbidity and mortality in patients with cancer or undergoing hematopoietic cell transplantation (HCT).1–3 Children with malignancies are highly susceptible to bacterial, fungal and viral infections as a consequence of intensive chemotherapy, impairment of the immune system, alterations in the body’s natural barriers, or presence of central venous catheters.1 Malignant bone tumors (MBT) in children are a rare group of tumors that accounts for 3–5% of pediatric cancer below 18 years of age.4 The two most frequent MBT are osteosarcoma (OSA) (51%) and Ewing sarcoma (ES) (45.9%).4 The current standard of management for ES is a combination treatment based on: neo-adjuvant chemotherapy, surgery, adjuvant chemotherapy with high-dose chemotherapy followed by autologous HCT (auto-HCT) in the case of IV stage, and radiotherapy.5,6 In patients with ES, the duration of combined oncological treatment ranges from 8–9 months for patients treated with high-dose chemotherapy followed by auto-HCT to 14 months in patients treated without auto-HCT. Treatment of OSA includes neo-adjuvant chemotherapy, surgery and adjuvant chemotherapy.5 Radiotherapy does not apply in OSA, and surgery might be recommended in cases of metastases.5,7 In patients with OSA, the duration of the combined oncological treatment ranges from 9 months for good-responders to 11–12 months for poor responders.
There are limited data on bacterial, fungal and viral infections in children with ES and OSA during intensive anticancer treatment. The objective of this study was to assess the epidemiology, risk factors and outcome of infections in children with MBT.
The study was designed as a retrospective multicenter nationwide cross-sectional analysis performed on behalf of the Polish Society of Pediatric Oncology and Hematology. Bacterial, fungal and viral infections diagnosed in the 6-year period (between January 1, 2012 and December 31, 2017), were reported by each of 13 participating centers and data were analyzed centrally by two independent researchers. In patients who underwent high-dose chemotherapy with HCT the observation was completed on the day before conditioning regimen to transplantation was started.
Only microbiologically documented bacterial infections (MDI) were analyzed. The following clinical categories of infections were diagnosed: bloodstream infections (BSI – presence of viable bacteria in bloodstream, combined with relevant clinical symptoms), urinary tract infections (UTI – presence of viable bacteria in urinary tract, combined with relevant clinical symptoms), skin and soft tissue infections (SSTI – primary or secondary cutaneous or soft tissue involvement due to any infectious bacterial agent) and gut infection (GI a combination of clinical symptoms from the gastrointestinal tract, and demonstration of bacteria and viruses in a specimen from the gastrointestinal tract by culture, antigen detection or PCR).1,8 Colonization was not included into this analysis. Bacteria were analyzed also with respect to their resistance profile: ESBL (extended-spectrum β-lactamase), AmpC (cephalosporinase AmpC), KPC (Klebsiella pneumoniae carbapenemase; class A carbapenemase), MBL (metallo-beta-lactamase; class B carbapenemase), OXA-48 (oxacillinase-48; class D carbapenemase) in Gram-negative rods, MRS (methicillin-resistant Staphylococcus), VRE (vancomycin-resistant Enterococcus), LRE (linezolid-resistant Enterococcus) and HLAR (high level aminoglycoside-resistant Enterococcus).1,9 Carbapenemases were detected by phenotypic and genotypic methods of identification, while other mechanisms of resistance by phenotypic methods. Multidrug-resistant (MDR) bacteria were defined as acquired non-denote resistance to at least two antibiotics used in empiric therapy or resistance to at least three antibiotic classes.1,10,11
The invasive fungal disease (IFD) was diagnosed according to European Organizations for Research and Treatment of Cancer/Mycoses study group (EORTC/MSG) criteria as proven, probable and possible.12,13 Infections of urinary tract with Candida species were diagnosed as probable. Viral infections were classified as latent or episodic.14 Respiratory tract infections were diagnosed with PCR, while GI infections with antigen-based tests. No routine pharmacological anti-infective prophylaxis was used in children with MBT during oncological treatment. Commonly accepted strategies were performed for empirical and targeted anti-infectious therapy with various agents.1,11,15,16 Neutropenia was defined as absolute neutrophil count lower than 1500/µL, and severe neutropenia <500/µL.
The chi-square test was used for categorical variables, and the Mann–Whitney U test for non-categorical variables. Odds ratio (OR) and confidence intervals (CI) were calculated for the differences in frequency of infections in patients. Cumulative incidences of viral, bacterial or fungal infections were calculated using competing risk analysis,17 starting from the day of diagnosis of MBT, to the day of the first infection. An event was defined as the diagnosis of a first specific infectious disorder. Death was considered as the competing event. The Kaplan–Meier method was used to determine infection-related mortality (IRM) and overall survival (OS).18 The risk factors were analyzed by multivariate logistic regression model in order to estimate hazard ratio (HR) with 95%CI. All reported p-values are two-sided; p<0.05 was considered as statistically significant.
Frequency of infection
Infectious complications occurred in 83 of 126 (65.8%) children with MBT including 59/70 (84.3%) with ES and 24/56 (42.8%) with OSA (OR=7.15, 95%CI=3.11–16.46, p<0.001); 43 boys and 40 girls, with a median age of 13.3 years (range: 16 days to 21 years). The median time from the diagnosis to the first infection (bacterial, fungal or viral) was 4.9 months (range 0.2–20). With respect to etiology, bacterial infections were diagnosed in 85.8% cases, fungal in 7.6% and viral in 6.7% of patients.
A total number of 103 episodes of bacterial infections occurred in 75 of 126 (59.5%) patients. They included 76/103 (73.8%) episodes in 52/70 (74.3%) patients with ES and 27/103 (26.2%) episodes in 23/56 (41.1%) patients with OSA (OR=4.1, 95%CI=1.9–8.8, p<0.001). The cumulative incidence of microbiologically-documented bacterial infections is shown in Figure 1. The median time from the diagnosis to the first episode of bacterial infection was 5.4 months (range 0.1–20 months). According to the site of infection, 33.0% of bacterial episodes were diagnosed as BSI, 31.1% as GI, 30.1% as UTI, and 6% as SSTI. All SSTI were associated with post-operative wound infection after surgical procedure. Site of infection and type of pathogen was shown in Table S1. Overall, 27.2% of the patients had more than one bacterial MDI, including two infections in 19.4% of the patients and three infections in 7.8% of the patients. None of the patients had more than three bacterial MDI. In 7.8% of episodes of infection more than one strain was isolated from the same specimen (seven episodes with two strains (UTI; 4, BSI; 2, SSTI; 1) and one episode (SSTI) with four strains). In 4.8% (5/103) episodes coexistence of infection in two sites was diagnosed (UTI/BSI – three episodes, UTI/SSTI – one episode, UTI/GI – one episode) (Table S2).
Table 1 Profile and antimicrobial resistance of Gram-negative bacteria, n (%)#
Table 2 Profile and antimicrobial resistance of Gram-positive bacteria, n (%)#
Figure 1 Bacterial infection incidence in patients with Ewing Sarcoma (ES) and osteosarcoma (OSA).
The most frequently diagnosed Gram-negative bacteria were Escherichia coli (40.8%), Klebsiella pneumoniae (25.3%) and Pseudomonas aeruginosa (11.3%). The most frequently diagnosed Gram-positive bacteria were Clostridium difficile (29.8%), S. epidermidis (21.3%) and S. aureus (14.9%) (Tables 1 and 2).
MDR pattern was present in 42.4% strains (56.3% Gram-negative and 21.3% Gram-positive) and was present in 100.0% of Enterobacter cloacae, 76.9% of K. pneumoniae, 66.7% of Acinetobacter baumannii and 61.9% of E. coli. The MRS occurred in 17.6% of all staphylococcal infections and vancomycin resistance was identified in 25.0% of enterococcal infections (Tables 1 and 2). MDR Gram-negative bacteria were more frequent in ES than in OSA (OR=3.4, 95%CI 1.15–10.1, p=0.027)
The median time of treatment of bacterial infections was 10 days (range 0–46 days) and was the same for ES and OSA patients. In patients with ES, IRM was 0.06 (95%CI=0.02–0.1) and did not differ from patients with OSA (0.15, 95%CI=0.07–0.23) (Figure 2). All deceased patients (six cases) died due to BSI (with coexistence of UTI in 33.3%) during neutropenia. In two of these patients MDR bacteria were cultured. During the study period we observed six deaths (6/126=4.8%) not related to infection, due to disease progression (5/70=7.1% with ES and 1/56=1.8% with OSA).
Figure 2 Infection related mortality (IRM) in patients with Ewing Sarcoma (ES) and osteosarcoma (OSA).
Cumulative incidence of IFD was 7.1% (nine patients), including proven IFD in 0.8% (one patient), probable 2.4% (three patients) and possible in 3.9% (five patients). None of the patients was diagnosed more than once for IFD; 22.2% of the IFD were reported in OSA (all possible), while 77.8% of the IFD in ES (p=ns). Clinical manifestation of IFD were: fungemia (one proven case, Candida guilliermondii in ES patient), UTI (two probable cases: one Candida kefyr, one Candida spp., both in ES), pneumonia (six possible IFD: four in ES, two in OSA). The diagnosis of possible fungal pneumonia was based on the chest HRCT in a neutropenic patient with cough and fever lasting >96 hours, with no improvement after broad-spectrum antibiotic therapy. The median time from the beginning of oncological treatment to IFD diagnosis was 6.2 months (range 0.6–20 months). The median time of anti-fungal treatment was 18 days (range 7–104 days): in 66.7% monotherapy was applied (fluconazole in three cases for 14–71 days, voriconazole in two cases for 33 and 104 days) and caspofungin in one case for 13 days). Combination therapy was used in the 33.3% of IFD (one case voriconazole + micafungin for 7 days, one case voriconazole + amphotericin B for 12 days and one case of caspofungin + amphotericin B for 36 days). All patients survived from IFD.
Cumulative incidence of viral infection was 6.4% (eight cases) including 0.8% of infections with HHV-6 (one case), 4.8% (six cases) of GI infections (five cases of rotavirus (three ES, two OSA) and one case of norovirus (ES)) and 0.8% (1 case, OSA) of influenza A. The median time from the diagnosis of MBT to diagnosis of viral infection was 2.4 months (range 0.7–13.4 months). HHV-6 infection was treated with ganciclovir for 8 days, and influenza A infection with oseltamivir for 5 days. In all cases symptomatic treatment was also implemented. No patient died due to the viral infections.
Risk factor analysis
The following risk factors predisposing to the incidence of infection were analyzed: primary diagnosis, sex, age (<13 years vs ≥13 years), and neutropenia. No risk factor was significant for bacterial, fungal and viral infections.
As no patient died from fungal or viral infection, the risk factor analysis for IRM was performed only for bacterial infections. The following risk factors were analyzed for bacteria-related IRM: primary diagnosis, sex, age (<13 years vs ≥13 years), type of bacteria (Gram-negative vs Gram-positive), neutropenia, bacteremia, pneumonia, time to infection (<5 months vs ≥5 months), and duration of treatment of infection (<11 days vs ≥11 days). The only significant risk factor for death was time to infection ≥5 months since primary diagnosis of bone tumor (Table 3, Figure 3). A trend towards higher mortality in patients ≥13 years was observed. All patients who died from bacterial infection had BSI and were neutropenic for a median of 7 days (range 2–14).
Table 3 Multivariate logistic regression analysis for risk factors for death from bacterial infections
Figure 3 Survival from bacterial infection with respect to time of infection (<5 months vs ≥5 months from the primary diagnosis of bone tumor).
Introduction of chemotherapy in order to intensify treatment together with the use of radiotherapy and surgical therapy of MBT has resulted in a significant increase in curability.5 The main adverse effects of intensification chemotherapy are neutropenia and infectious complications.1 There are only a few reports on infectious complications in children with MBT (apart from case reports) which focus on infectious complications in the perioperative period, prosthetic infections or surgical site infections.19–22
We report the results of a study on epidemiology, risk factors and outcome of infections in children with ES or OSA including bacterial, fungal and viral episodes analyzed over a period of 6 years. All reported patients were treated with the same therapeutic protocols, using comparable supportive therapies.
The highest risk of infectious complications related with the applied chemotherapy occurs in patients with acute leukemia, non-Hodgkin’s lymphoma and after HCT.1,5 We observed high incidence of infectious complications in children with MBT, especially with ES. More advanced stages at diagnosis, more intensive chemotherapy with more immunosuppressive reagents and more MDR bacteria were the possible reason of higher incidence of infectious complications in ES than OSA. Analysis of neutropenic fever after the VIDE cycles in patients treated according to EURO-EWING 99 was performed by Penel-Page et al.23 It was shown that patients treated in the adult services were less frequently hospitalized with shorter length of stay, without differences in survival, number of documented infections, transfusions, dose modification, delay in chemotherapy, or stay in the intensive care unit.23
Angelini et al24 analyzed infectious complications in patients after surgical resection due to bone tumors. They found that 20% of patients (15% without bone reconstruction and 26% with bone reconstruction) had a deep infection develop at a mean time of 8 months from diagnosis. In the multivariate analysis, only bone surgery was a risk factor for the occurrence bacterial complications.24 Longhi et al25 found that among patients with OSA, infection of the central catheter with fever were found in 5.6% of them. Authors suggest that the most common complications of the use of central venous catheters were infection and venous thrombosis whereas pulmonary septic emboli were rare.25 We observed that only 6% of infections were associated with post-operative wound infection after surgical procedure, while BSI and the UTI were the most frequent type of infection complication.
Additionally, in the analysis of the Polish Pediatric Group for Solid Tumors performed 20 years ago, including infections in patients treated for OSA between 1989 and 1998 according to the protocol OS-SFOP-94, deaths were observed in 5 of 28 treated patients, however only one child died due to infectious complications, while the other four died due to non-infectious complications (three with disease progression/relapse, one because of treatment refusal).26
In the other study, among 125 children and young adults with OSA chronic localized infections were determined in 4.8% of patients.27 More amputations (p<0.001) were necessary in infected patients due to uncontrolled infection. The 5‑year overall survival rate and event‑free survival rate in infected patients were 100%, which were significantly higher than that of the non‑infected patients, of whom the rates were 54% and 43% respectively (p=0.01). Authors suggest that association between infection and survival rate of OS patients remains to be elucidated, because of the wide spectrum of immune cells and transducers which have shown potential in the treatment of OSA. On the other hand, risk factors analysis showed that only tumor metastasis was an independent negative risk factor for survival (p<0.001).27 In our study in the multivariate analysis for risk factors of death from bacterial infection we found that the only significant risk factor was time to infection ≥5 months since primary diagnosis of MBT and severe neutropenia was present in all these cases for a period of 2–14 days. However, neutropenia was not a significant factor in the multivariate analysis, as most bacterial infections developed during the neutropenic phase. High intensity of the chemotherapy followed by myelosuppression could be the reasons for this observation.
We found that the overall cumulative incidence of bacterial infections in ES and OSA (74.3% and 41.1%, respectively) was higher than previously reported in patients with acute lymphoblastic leukemia (ALL) (40.9%), Non-Hodgkin lymphoma (NHL) (37.0%) and Hodgkin’s disease (HD) (12.7%), while in acute myelogenous leukemia (AML) it was comparable (58.0%).1 We have shown that IFD incidence in patients with MBT was lower (7.1%) than in patients after allo-HCT (30.6%) and auto-HCT (17.1%) and also in patients with AML (43.0%), ALL (13.0%) and NHL (10.2%), but higher than in patients with HD (1.8%).1 Comparing to HCT patients,1 MDR bacteria were less frequent in patients with MBT (76.8% vs 42.4% respectively), what can be explained by usually longer and more intensive chemotherapy preceding HCT.
Although the present study is inherent to several limitations of observational research, including retrospective design, its strong points are that it is a multicenter, nationwide long-term study carried out on a large homogeneous group of patients treated in the same way in all centers. Additionally, to our knowledge, this is the first such comprehensive study of infectious complications in children with MBT.
Infections in children with MBT occurred with high frequency, especially in patients with ES. The most frequent were bacterial infections, while fungal and viral infections were episodic. Among the bacterial infections, BSI, UTI and GI occurred with similar frequency. The MDR bacteria occurred in 42.4% of episodes, and mainly they were Gram-negative bacteria and were more frequent in ES patients than OSA patients. All patients who died from infection were in severe neutropenia and died due to BSI. The only significant risk factor for death was time to infection ≥5 months since primary diagnosis of MBT in patients with neutropenia. Presented data provide new insights into infections in children with MBT and underline that there is an increased risk of death related to infection after 5 months of intensive chemotherapy.
ALL, acute lymphoblastic leukemia; AML, acute myelogenous leukemia; AmpC, cephalosporinase AmpC; auto-HCT, autologous hematopoietic cell transplantation; BSI, bloodstream infections; CI, confidence intervals; EORTC/MSG, European Organizations for Research and Treatment of Cancer/Mycoses study group; ES, Ewing sarcoma; ESBL, extended-spectrum β-lactamase; GI, gut infection; HCT, hematopoietic cell transplantation; HD, Hodgkin’s disease; HHV-6, human herpesvirus 6; HLAR, high level aminoglycoside-resistant Enterococcus; IFD, invasive fungal disease; IRM, infection-related mortality; KPC, Klebsiella pneumoniae carbapenemase, class A carbapenemase; LRE, linezolid-resistant Enterococcus; MBL, metallo-beta-lactamase, class B carbapenemase; MBT, malignant bone tumors; MDI, microbiologically documented bacterial infections; MDR, multidrug resistant; MRS, methicillin-resistant Staphylococcus; NHL, Non-Hodgkin lymphoma; OR, odds ratio; OS, overall survival; OSA, osteosarcoma; OXA-48, oxacillinase-48, class D carbapenemase; SSTI, skin and soft tissue infections; UTI, urinary tract infections; VRE, vancomycin-resistant Enterococcus.
This study was conducted in accordance with the Declaration of Helsinki and approved by the ethics committee of Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun. All patient data were analyzed in anonymity. As neither individual data were published nor any intervention was performed on patients, patient consent was waived by the ethics committee.
The authors thank the following persons for their support and contribution to the creation of this paper: Magdalena Dziedzic, Monika Pogorzala, Ewa Demidowicz, Natalia Bartoszewicz, Mariusz Wysocki, Bozenna Dembowska-Baginska, Katarzyna Dzierzanowska-Fangrat, Walentyna Balwierz, Bernarda Kazanowska, Jacek Wachowiak, Elzbieta Adamkiewicz-Drozynska, Maryna Krawczuk-Rybak, Jaroslaw Peregud-Pogorzelski, Wojciech Mlynarski, Mariola Woszczyk, Wanda Badowska, Grazyna Sobol-Milejska, Radoslaw Chaber. The authors thank anonymous reviewers for their suggestions to improve the article.
KC and JS were responsible for the concept of the study and design. KC was the major contributor in writing the paper. All authors contributed toward data collection, data analysis, critically revising the paper, gave final approval of the version to be published and agree to be accountable for all aspects of the work.
The authors report no conflicts of interest in this work.
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Table S1 Site of infection and type of pathogen in patients with ES and OSA
Table S2 Site of infection and type of pathogen in coexistent infections
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