Back to Journals » International Journal of Nanomedicine » Volume 7

In vitro perforation of human epithelial carcinoma cell with antibody-conjugated biodegradable microspheres illuminated by a single 80 femtosecond near-infrared laser pulse

Authors Terakawa M, Tsunoi, Mitsuhashi

Received 13 March 2012

Accepted for publication 5 April 2012

Published 28 May 2012 Volume 2012:7 Pages 2653—2660


Review by Single-blind

Peer reviewer comments 2

Mitsuhiro Terakawa, Yasuyuki Tsunoi, Tatsuki Mitsuhashi

Department of Electronics and Electrical Engineering, Keio University, Yokohama, Kanagawa, Japan

Abstract: Pulsed laser interaction with small metallic and dielectric particles has been receiving attention as a method of drug delivery to many cells. However, most of the particles are attended by many risks, which are mainly dependent upon particle size. Unlike other widely used particles, biodegradable particles have advantages of being broken down and eliminated by innate metabolic processes. In this paper, the perforation of cell membrane by a focused spot with transparent biodegradable microspheres excited by a single 800 nm, 80 fs laser pulse is demonstrated. A polylactic acid (PLA) sphere, a biodegradable polymer, was used. Fluorescein isothiocyanate (FITC)-dextran and short interfering RNA were delivered into many human epithelial carcinoma cells (A431 cells) by applying a single 80 fs laser pulse in the presence of antibody-conjugated PLA microspheres. The focused intensity was also simulated by the three-dimensional finite-difference time-domain method. Perforation by biodegradable spheres compared with other particles has the potential to be a much safer phototherapy and drug delivery method for patients. The present method can open a new avenue, which is considered an efficient adherent for the selective perforation of cells which express the specific antigen on the cell membrane.
Keywords: femtosecond laser, drug delivery, transfection, biodegradable polymer

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]


Readers of this article also read:

Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples

Veerapandian M, Seo YT, Shin H, Yun K, Lee MH

International Journal of Nanomedicine 2012, 7:6123-6136

Published Date: 18 December 2012

Comparing the effects of nano-sized sugarcane fiber with cellulose and psyllium on hepatic cellular signaling in mice

Wang ZQ, Yu Y, Zhang XH, Floyd ZE, Boudreau A, Lian K, Cefalu WT

International Journal of Nanomedicine 2012, 7:2999-3012

Published Date: 18 June 2012

Preventing postoperative abdominal adhesions in a rat model with PEG-PCL-PEG hydrogel

Yang B, Gong C, Zhao X, Zhou S, Li Z, Qi X, Zhong Q, Luo F, Qian Z

International Journal of Nanomedicine 2012, 7:547-557

Published Date: 2 February 2012

Microemulsion-based novel transdermal delivery system of tetramethylpyrazine: preparation and evaluation in vitro and in vivo

Zhao JH, Ji L, Wang H, Chen ZQ, Zhang YT, Liu Y, Feng NP

International Journal of Nanomedicine 2011, 6:1611-1619

Published Date: 9 August 2011

Optimizing particle size for targeting diseased microvasculature: from experiments to artificial neural networks

Boso DP, Lee S, Ferrari M, Schrefler BA, Decuzzi P

International Journal of Nanomedicine 2011, 6:1517-1526

Published Date: 19 July 2011

Integrin αvβ3-targeted gold nanoshells augment tumor vasculature-specific imaging and therapy

Huan Xie, Parmeswaran Diagaradjane, Amit A Deorukhkar, et al

International Journal of Nanomedicine 2011, 6:259-269

Published Date: 27 January 2011