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In vitro antioxidant activity and in vivo antifatigue effect of layered double hydroxide nanoparticles as delivery vehicles for folic acid

Authors Qin L, Wang W, You S, Dong J, Zhou Y, Wang J

Received 14 September 2014

Accepted for publication 1 November 2014

Published 4 December 2014 Volume 2014:9(1) Pages 5701—5710


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 4

Editor who approved publication: Dr Thomas Webster

Lili Qin,1,* Wenrui Wang,2,* Songhui You,1 Jingmei Dong,1 Yunhe Zhou,1 Jibing Wang1

1Department of Physical Education, 2School of Life Science and Technology, Tongji University, Shanghai, People’s Republic of China

*These authors contributed equally to this work

Abstract: Folic acid antioxidants were successfully intercalated into layered double hydroxides (LDH) nanoparticles according to a previous method with minor modification. The resultant folic acid-LDH constructs were then characterized by X-ray powder diffraction and transmission electron microscopy. The in vitro antioxidant activities, cytotoxicity effect, and in vivo anti­fatigue were examined by a series of assays. The results showed that folic acid-LDH antioxidant system can scavenge 1,1-diphenyl-2-picrylhydrazyl and hydroxyl free radicals and chelate pro-oxidative Cu2+. The in vitro cytotoxicity assays indicated that folic acid-LDH antioxidant system had no significant cytotoxic effect or obvious toxicity to normal cells. It also prolonged the forced swimming time of the mice by 32% and 51% compared to folic acid and control groups, respectively. It had an obvious effect on decreasing the blood urea nitrogen and blood lactic acid, while increasing muscle and hepatic glycogen levels. Therefore, folic acid-LDH might be used as a novel antioxidant and antifatigue nutritional supplement.

Keywords: antioxidant supplementation, free radicals, biomaterials, drug delivery


The classic “radical theory” indicates that intense exercise can produce an imbalance between the body’s oxidation and antioxidation systems. The accumulation of reactive free radicals will lead to oxidative stress which can cause oxidative damage to bimolecular such as protein, lipid, and DNA.1 It also can induce injury to tissues or organs and is one of the reasons for fatigue.2,3 Fatigue is a physiological phenomenon that arises from physical stress or exhaustive exercises leading to the reduction of physical endurance capacity.4 Therefore, supplementing with antioxidants has been considered to have an important role in reducing the degree of fatigue caused by free radical and oxidative stress.5

Folic acid is a vitamin of the B group. It can be converted into a coenzyme N5,N10-dimethylene-5,6,7,8-tetrahydrofolate in vivo through a series of enzymatic transformations. It has purine and pyrazine type rings and two amino acids on the pyrazine ring. Its lactim form has a hydroxyl group on the purine type ring. When folic acid reacts with oxidizing free radicals, this hydroxyl group can play an important role in inhibiting the oxidation effect.6 Therefore, some researches have reported that folic acid possesses a significant antioxidant effect and reduces oxidative stress.7,8 However, folic acid, like other natural antioxidants, is limited by poor stability, short half-life in vivo, low bioavailability, and is easily degraded by proteolytic or gastrointestinal enzymes. Therefore, using nanomaterials as an efficient delivery system for antioxidants can solve these problems and improve clinical therapy.

Layered double hydroxides (LDHs) as one of the inorganic delivery nanoparticles have recently been receiving considerable attentions.9,10 They possess some promising advantages such as low cost, good biocompatibility, low toxicity to mammalian cells, controlled release property, and full protection for loaded drugs. Many LDH compounds intercalated with beneficial organic anions such as DNA,11,12 amino acids,13 anionic polymers,14 pesticides,15 and drugs1619 have been prepared successfully. Furthermore, the antioxidant intercalated into LDH delivery can be used as a nanomaterial antioxidant supplementation, thereby expanding its range of applications.

In our previous research, we reported that folic acid-LDH nanohybrids could be used as a reservoir of folic acid molecules.20 In the present study, folic acid-LDH was prepared according to a previous method with some modifications. Our goal here is to examine the in vitro antioxidative capacity of folic acid-LDH as well as its in vivo antifatigue effects.

Materials and methods


Mg(NO3)2·6H2O, Al(NO3)3·9H2O, folic acid, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) were purchased from China National Medicine Group, Shanghai Chemical Reagents Company (Shanghai, People’s Republic of China), and were used without further purification. RPMI-1640, fetal calf serum, penicillin G, streptomycin, and trypsin were obtained from GIBCO BRL (Grand Island, NY, USA). Dimethyl sulfoxide and [3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] (MTT) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Deionized water was decarbonated by boiling before use in all applications.


All the in vivo tests were carried by Academy of Experimental Animal Center of Tongji University (Shanghai, People’s Republic of China), which obtained the permission for performing the research protocols and all animal experiments were approved by the ethics committee of Tongji University. Sixty male Kunming mice (15–22 g), specific pathogen-free grade, were purchased from Shanghai Laboratory Animal Centre (SLAC). Animals were allowed to adapt to their surroundings for 1 week before starting the experiments. Mice were housed five to six per cage at room temperature (22°C±2°C) and moderate humidity (50%±10%), with a 12-/12-hour light/dark cycle. The noise was <60 dB. They were fed a balanced murine diet with drinking water available ad libitum provided by the Academy of Experimental Animal Center of Tongji University.

Sixty mice were randomly divided into six groups with ten mice each: two control groups, two folic acid treatment groups, and two folic acid-LDH treatment groups. The control groups were administered distilled water at 10 mL/kg of body weight. The other groups were administered folic acid at 5 mg/kg of body weight. The amount of folic acid was adjusted to be equal to that of the folic acid-LDH treatment group. Water, folic acid, and folic acid-LDH were administered for 28 days. The forced swimming test was conducted on the last day and corresponding biochemical parameters were measured.

Preparation of folic acid-LDH

Folic acid-LDH was prepared according to a previous co-precipitation method with some modifications.20 A mixed solution (40 mL) of 0.17 M NaOH and 0.1 M folic acid was stirred at room temperature for 10 minutes, and then a solution (10 mL) containing 0.3 M Mg(NO3)2 and 0.1 M Al(NO3)3 was added. The mixture was continuously vigorously magnetically stirred under N2 for 3 hours. The resultant precipitates were centrifuged (4,500 rpm, 15 minutes) and washed twice with deionized water, and then dispersed in 40 mL of double distilled water and treated in an autoclave at 50°C for 12 hours. The LDH solid was harvested by filtering and vacuum drying overnight. The product was denoted as folic acid-LDH.


X-ray powder diffraction (XRD) patterns were recorded on a Rigaku Miniflex diffractometer using CuKa radiation (λ=0.154060 nm, 40 kV, 40 mA, step of 0.0330°). The average particle size (z-average size) and size distribution were measured using photon correlation spectroscopy (PCS) (LS230; Beckman Coulter) at 25°C under a fixed angle of 90° in disposable polystyrene cuvettes. The measurements were recorded using a He–Ne laser of 633 nm. Zeta potential distribution and polydispersity index of the nanoparticles were analyzed by Nano ZS (Malvern Instruments, Malvern, UK). Transmission electron micrographs were taken using a JEOL (Tokyo, Japan) 1230 transmission electron microscope (TEM). Ultraviolet (UV)/visible (VIS) absorption spectra were measured on a CARY 50 spectrophotometer.

In vitro antioxidant activities of folic acid-LDH

Hydroxyl radical scavenging activity assay

The hydroxyl radical scavenging activity assay was performed according to the method by de Avellar et al with some modifications.21 The solution contained 1,10-phenanthroline (5.0 mM, 600 μL), FeSO4 (5.0 mM, 600 μL), and ethylenediaminetetraacetic acid (15 mM, 600 μL) dissolved in sodium phosphate buffer (0×10−3 mg/mL to 20×10−3 mg/mL). Then, folic acid, folic acid-LDH (0–20×10−3 mg/mL), and H2O2 (0.01%) were added. The mixture was incubated at 37°C for 60 minutes, and the absorbance was measured by UV/VIS absorption spectra on a CARY 50 spectrophotometer at 536 nm. The result was determined using the following equation:


where AS is the absorbance of the sample; A0 is the absorbance of the blank solution using distilled water instead of sample; and AC is the absorbance of a control solution in the absence of H2O2.

DPPH radical scavenging activity assay

The scavenging activity of folic acid-LDH on DPPH free radicals was measured according to the method of Shimada et al with minor modification.22 Two milliliters of folic acid and folic acid-LDH (0×10−3 mg/mL to 6×10−3 mg/mL) were mixed with 2.0 mL of 0.10 mM DPPH that was dissolved in 95% ethanol. The mixture was then shaken vigorously and kept for 30 minutes in the dark at room temperature. The absorbance of the resulting solution was recorded at 517 nm. A lower absorbance represents a higher DPPH scavenging activity. The scavenging activity was calculated using the following equation:


here, ADPPH sample is the value for the 2 mL of sample solution mixed with the DPPH solution; ASample control is the value for the 2 mL of sample solution mixed with the 2 mL of 95% ethanol; and ADPPH blank is the value for the 2 mL of 95% ethanol mixed with the DPPH solution.

Cupric ion chelating activity assay

The chelating effect of folic acid-LDH on pro-oxidative Cu2+ was investigated according to Zhu et al.23 In the test, 1 mL of 2 mM CuSO4 was mixed with 1 mL of pyridine (pH 7.0) and 0.1% pyrocatechol violet. After the addition of 1 mL of folic acid and folic acid-LDH (0–6×10−3 mg/mL), the blue color disappeared because of the dissociation of Cu2+. After reacting for 5 minutes, it was recorded by measuring the absorbance at 632 nm. An equivalent volume of distilled water instead of the sample was used for the blank. The Cu2+ chelating activity were calculated as:


where AS is the absorbance of the sample and A0 is the absorbance of the blank solution using distilled water instead of sample.

In vitro cytotoxicity effect of folic acid-LDH

GES-1 cells derived from human gastric mucosa epithelium were obtained from Shanghai institute of biochemistry and cell biology (Shanghai, People’s Republic of China). Cells were routinely cultured at 37°C in 5% CO2 atmosphere in the flasks containing 10 mL of Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 μg/mL streptomycin. At 80%–90% confluence, cells were disassociated using trypsin–ethylenediaminetetraacetic acid and plated at a density of approximately 2×104 cells per well in a 96-well plate. The number of viable cells was determined by MTT assay with 3-(4,5-dimethylthiazole-2-yl)-2,5-phenyltetrazolium bromide. After incubation at 37°C in a 5% CO2 humid atmosphere for 24 hours, triplicate wells were treated with folic acid, folic acid-LDH, and LDH of various concentrations (5, 10, 20, and 40 μg/mL). The control cultures were grown in growth medium only. Then, the incubation was continued as indicated above for 4 hours. For the LDH-treated wells, the amount of LDH was adjusted to be equal to that of folic acid-LDH-treated wells. Subsequently, each well was incubated with 20 μL (5 mg/mL) of MTT dye solution for 4 hours at 37°C. After removal of the MTT solution, cells were treated with 150 μL dimethyl sulfoxide and the absorbance at 490 nm was quantified using a microplate reader (ELX 800 UV; BIO-TEK, Vermont, USA). Cell viability was calculated by means of the following formula:


In vivo antifatigue effect of folic acid-LDH

Effect on swimming time to fatigue

After 28 days, ten mice were taken from each group for the forced swimming test. The procedure was used according to the previous study with some modification.24 Briefly, 30 minutes after the final treatment with folic acid, folic acid-LDH or distilled water, the mice were placed individually in a swimming pool (50×50×40 cm) filled with water to a depth of 30 cm and the water temperature was kept at 25°C±1°C. A lead sinker (10% of the body weight) was attached to the tail root of each mouse. The mice were assessed to be exhausted when they failed to rise to the surface within 10 seconds. The swimming time was recorded immediately. In the last stage, the mice were then removed from the water, dried with a paper towel, and returned to their original cages. The pool water was replaced after each session. The swimming time to exhaustion was used as the index of the forced swimming capacity.

Determination of biochemical parameters related to fatigue

After the final treatment with folic acid, folic acid-LDH or distilled water, the mice were allowed to rest for 30 minutes. Then they were placed in the swimming tank. After swimming for 30 minutes without loads, the whole blood was collected via retro-orbital venipuncture, and serum was prepared by centrifugation at 4,000 rpm at 4°C for 15 minutes. Then, the content of blood urea nitrogen (BUN) and blood lactic acid (BLA) were determined. The liver and gastrocnemius muscle were collected to be made into 10% homogenates with saline as soon as possible. All of the biochemical parameters were determined using the commercial diagnostic kits according to the procedures provided by the Nanjing Jiancheng Bioengineering Institute.

Statistical analysis

For statistical analysis, the data were presented as the mean ± standard deviation of three independent experiments. One-way analysis of variance followed with t-test was performed using the SPSS program, and the levels of significance were represented in each result.

Results and discussion

Preparation and characterization of folic acid-LDH

The powder XRD patterns of folic acid-LDH sample was compared to the pristine LDH pattern (Figure 1). The patterns of LDH and folic acid-LDH are typical of lamellar materials. They are characterized with basal reflections, associated with harmonics at low 2θ angles, and weaker nonbasal reflections at higher angles.25 As shown in Figure 1A, the pristine LDH is well crystallized, showing a typical XRD pattern for LDH-NO3 with the sharp (003) and (006) basal peaks. The intercalation of folic acid can lead to a significant increase in the interlayer space. For the pattern of folic acid-LDH in Figure 1B, the reflection peaks shift to lower angles with becoming weaker and broader. These reflections could be attributed to folic acid intercalation to LDH layer with the basal spacing from 0.88 to 1.18 nm (Figure 2). Assuming a thickness of 0.48 nm for the brucite-like layer of LDH, the gallery height in folic acid-LDH became 0.70 nm, which is shorter than the molecular length of folic acid (~2.15 nm).20,26 The reason for the discrepancy between the calculated gallery height (0.70 nm) and estimated molecular length (~2.15 nm) is the strong electrostatic interactions between folic acid anions and LDH hydroxide layers. It also can be deduced that the folic acid molecules are arranged in a tilted longitudinal monolayer, with tilting angles of approximately 70°. As listed in Table 1, the amount of folic acid intercalated into the LDH was calculated using a UV–VIS spectrophotometer and its drug loading was about 32.4% (w/w). Thus, in this study, the preparation procedure offers a much higher drug-loading amount compared to our previous study.20

Figure 1 Powder X-ray diffraction patterns for LDH and folic acid-LDH.
Notes: (A) LDH and (B) folic acid-LDH. The pointed arrow indicates the new basal diffractions attributed to the folic acid intercalated into LDH phase.
Abbreviation: LDH, layered double hydroxide.

Figure 2 Schematic model of layered double hydroxide structure before and after folic acid intercalation.

Table 1 The d-values of nanohybrid and folic acid loading
Note: – indicates no data.
Abbreviation: LDH, layered double hydroxide.

Physicochemical characteristics of folic acid-LDH

TEM samples were prepared by adding 300 mL of LDH and folic acid-LDH slurry (0.5 mg/mL) to 15 mL of deionized water followed by probe sonication of the solution for 2 minutes. A drop of the solution was then placed on a holey carbon copper grid and air dried. As shown in Figure 3A and B), Mg/Al-LDH was observed as compact, nonporous crystallites with a hexagonal plate-like shape and a diameter of approximately 50 nm. When the folic acid intercalated into the inorganic host, the shape of the nanohybrid changed from regular hexagons to ellipses, and the crystal size was increased to a diameter range of 80–90 nm. The particle size and distribution dynamics of folic acid-LDH were investigated by PCS. The mean particle size of folic acid-LDH is narrowly distributed with a nominal hydrodynamic diameter of 95.3 nm (Figure 3C), and the zeta potentials are 27.5 mV with a polydispersity index of 0.317±0.005 (Figure 3D). The size of particles measured by PCS is larger than those estimated using TEM. This is because PCS is used for hydrodynamic diameter determination, while TEM is used for characterization of particles in the dried state.27

Figure 3 TEM images of nanohybrids.
Notes: (A) LDH crystals (B) folic acid-LDH nanohybrid crystals. Particle size distribution (C) and zeta potential distribution (D) of folic acid-LDH nanoparticles.
Abbreviations: LDH, layered double hydroxide; TEM, transmission electron microscope.

In vitro antioxidant activities of folic acid-LDH

In vitro antioxidant activities of folic acid-LDH were evaluated by several antioxidant activity-determining assays based on different active mechanisms. The different free radical systems including hydroxyl radical, DPPH, and Cupric ion were employed in this study.

Hydroxyl radicals (OH·) are one of reactive oxygen species generated in the human body. They are the most destructive reactive oxygen species and can be formed from superoxide anion and hydrogen peroxide in the presence of metal ions such as copper or iron.28 Due to their high level of reaction activity, they can induce oxidative damage to almost all important biomolecules including amino acids, DNA, polyunsaturated fatty acids, and proteins. This can lead to physiological disorders and disease.29 The hydroxyl radical scavenging ability of folic acid-LDH with different concentrations is shown in Figure 4. As the folic acid-LDH concentration increased, the hydroxyl radical scavenging activities exhibited an increasing tendency to higher scavenging activity. When the concentration reached 20×10−3 mg/mL, it showed 83.86% hydroxyl radical scavenging activity, which is obviously higher than that of folic acid.

Figure 4 Hydroxyl radical scavenging activity of folic acid and folic acid-LDH.
Note: Results represent the means of three independent experiments.
Abbreviation: LDH, layered double hydroxide.

The relatively stable DPPH radical was widely used to test the ability of compounds to act as free radical scavengers or hydrogen donors to determine the antioxidant activity. This method has been recommended as an easy and accurate method of measuring the antioxidant activity.30 Figure 5 shows the DPPH radical scavenging capacity of folic acid-LDH in comparison with folic acid as reference. The results suggest significant antioxidant activity in a concentration-dependent manner. Folic acid-LDH shows an excellent DPPH radical scavenging activity of 91.71%, while folic acid offers 78.23% inhibitory effect at the concentration of 6×10−3 mg/mL.

Figure 5 DPPH radical scavenging activity of folic acid and folic acid-LDH.
Note: Results represent the means of three independent experiments.
Abbreviations: DPPH, 1,1-diphenyl-2-picrylhydrazyl; LDH, layered double hydroxide.

The radical system used for antioxidant evaluation may influence the experimental results, so two or more radical systems are required to investigate the antioxidant capacities.31 The folic acid-LDH was analyzed for pro-oxidative Cu2+ chelating effect to better examine its antioxidant properties. As shown in Figure 6, both folic acid and folic-LDH suggest significant Cu2+ chelating activity in a concentration-dependent manner. In this assay, folic acid-LDH shows the highest chelating activity of 73.44% at the concentration of 6×10−3 mg/mL. On the other hand, folic acid shows about 65.89% chelating effect of pro-oxidative Cu2+.

Figure 6 Cupric ion chelating activity of folic acid and folic acid-LDH.
Note: Results represent the means of three independent experiments.
Abbreviation: LDH, layered double hydroxide.

In summary, firstly, all antioxidant activity assays indicate that folic acid does not lose its antioxidant properties after the intercalation into LDH layers. Secondly, it is evident that folic acid loaded into LDH exhibits an obvious improved antioxidant capacity in comparison with the same concentration of free-drug folic acid. Therefore, folic acid-LDH nanoparticles possess in vitro antioxidant abilities that imply their potential to reduce the lipid peroxidation and reinforce antioxidant defense of free radicals. Moreover, they may have the potential ability to reduce oxidative stress in vivo and protect the body from fatigue.32

Cytotoxicity study of folic acid-LDH

The cytotoxicity of pure folic acid and folic acid-LDH were evaluated in GES-1 using MTT tetrazolium assay. The yellow tetrazolium MTT was reduced by metabolically active cells, in part by the action of hydrogenase enzymes to generate reducing equivalents such as NADH and NADPH. The resulting intracellular purple formazan was solubilized and quantized by spectrophotometric means. Figure 7 shows the effect of folic acid and folic acid-LDH on the proliferation of GES-1 cells at various concentrations. Both pure drug folic acid and folic acid-LDH nanoparticles do not show an obvious toxicity to GES-1 cells owing to the fact that the rate of living cells is maintained at 90% after incubation for 48 hours at each of the tested concentrations. Therefore, this confirms that folic acid-LDH is hardly toxic to GES-1 cells and can serve as a safe drug-delivery system in the cellular system.

Figure 7 Cell viability of LDH, folic acid, and folic acid-LDH on the growth of GES-1 cells.
Notes: The cells have been treated with one of these three agents for 48 hours at concentrations of 5, 10, 20, and 40 μg/mL. Results represent the means of three independent experiments and error bars represent the standard error of the mean. N=3, *P<0.05; **P<0.001. All entries showed significant difference with the control group according to the Student’s t-test.
Abbreviation: LDH, layered double hydroxide.

In vivo antifatigue capacity of folic acid-LDH

Effect of folic acid-LDH on prolonged swimming time

The forced swimming test is one of the most commonly used animal models for evaluating physical endurance capacity as it provides high reproducibility. Reduced susceptibility to fatigue is correlated with longer swimming times.33 The forced swimming time of mice is shown in Figure 8. Compared to the control groups, oral administration of both folic acid and folic acid-LDH significantly (P<0.05) prolong the time to exhaustion. The average swimming time of the folic acid-LDH-mice group was 13.12 minutes, which is an increase of 51% compared with the control group. Meanwhile, the forced swimming time of the folic acid groups was 9.42 minutes, which is 32% longer than that of the control groups. The swimming-time data suggest that the administration of folic acid-LDH could prolong the swimming time for exhaustion, indicating that folic acid-LDH possesses an antifatigue effect that could elevate exercise endurance.34

Figure 8 Effect of folic acid and folic acid-LDH on the swimming time in mice.
Notes: Results present means of three independent experiments and error bars represent the standard error of the mean. N=3, **P<0.001. All entries showed significant difference compared with the control group.
Abbreviation: LDH, layered double hydroxide.

Effect of biochemical parameters related to fatigue

BUN is a product of energy metabolism representing normally kidney function, which is the metabolism outcome of protein and amino acid fatigue.35 Urea as the end product of protein metabolism and is formed in the liver. In the process of digestion, protein is broken down into small peptides and amino acid. The amino acid nitrogen is removed as NH4+, and the rest of the molecule is used to produce energy or other substances for the cell demand.36 As BUN is a sensitive index of fatigue status, it has a positive correlation between urea nitrogen in vivo and exercise tolerance, which means that the worse the body is adapted for exercise tolerance, the more significantly the urea nitrogen level increases.37 As shown in Figure 9, the levels of BUN in folic acid and folic acid-LDH groups are lower than those in control groups. The BUN contents in the folic acid groups were significantly lower by 10.0% compared to the control groups, while those in the folic acid-LDH groups were decreased by 19.1%, indicating that folic acid-LDH may reduce protein metabolism and enhance endurance.38

Figure 9 Effect of folic acid and folic acid-LDH on BUN, BLA, hepatic glycogen, and muscle glycogen contents of mice.
Notes: Results represent means of three independent experiments and error bars represent the standard error of the mean. N=3, **P<0.001 as compared with the control group.
Abbreviations: BLA, blood lactate acid; BUN, blood urea nitrogen; LDH, layered double hydroxide.

BLA is the glycolysis product of carbohydrates under an anaerobic condition. It results from muscles doing high-intensity exercise in a short time. The excess lactic acid can be removed and converted to glucose via a gluconeogenesis pathway, then the excess glucose will be stored as hepatic glycogen.39 With the intense exercises increased, the higher level of lactic acid will cause an increased H+ concentration and change the internal pH value. It will induce many negative side effects on cells, organs, and physiological processes that ultimately result in fatigue.40 Therefore, the blood lactate acid is one of the important indexes to reflect the fatigue degree. As per the results shown in Figure 9, it is found that folic acid and folic acid-LDH can reduce the BLA level by 21.3% and 30.5%, respectively. The results indicate that folic acid-LDHs have a better effect on reducing BLA and can protect the body from appearance of fatigue.

It has been established that the effect of energy stored as glycogen is one of the most important factors in determining the capacity for prolonged exercise.41 When muscle glycogen stores are depleted during exercise, “the content of glycogen stores lasts longer in liver than in muscle.42 Energy for exercise is derived initially from the utilizing of glycogen, then from the glycogen stored in liver, and later from non-esterified fatty acids.43 Therefore, liver and muscle glycogen are sensitive indices related to fatigue. The data in Figure 9 reveals that the levels of hepatic glycogen and muscle glycogen in both folic acid and folic acid-LDH groups are much higher than those in control groups. The folic acid-LDH groups show an obvious increased content level compared to the folic acid groups. The data indicates that folic acid-LDH can increase the storage of hepatic glycogen leading to enhanced exercise endurance and improved energy metabolism activity.

Many studies have reported that folic acid as a vitamin of the B group reacts with the free radicals that play an important role on antioxidant effect.44 The in vivo antifatigue assays indicate that both folic acid and folic acid-LDH groups showed obvious increased effects on biochemical parameters related to fatigue compared to the control groups. Moreover, folic acid intercalates into LDH delivery are more effective in decreasing BUN and BLA, and increasing muscle and hepatic glycogen level compared to the folic acid groups. We propose the following mechanism: firstly, the LDH nanomaterials have a sustained release profile which can help increase the practical delivery of folic acid and the drug molecules. The folic acid is more stabilized when it is entrapped in LDH, which can protect it from degradation in the biochemical reaction process.17 Secondly, loading LDH nanoparticles with antioxidants would improve cellular drug uptake and retention.45,46 Therefore, the folic acid-LDH delivery system may be used as a potential antioxidant supplement for a broad range of exercise endurance and antifatigue applications. This research provides an important basis for developing nanomaterial nutritional supplements suitable for antioxidant and antifatigue effects.


In this research, the folic acid-LDHs were successfully prepared with 32.4% (w/w) drug loading. The composition, structure, particle shape, and size of the nano-antioxidants were also characterized. The in vitro antioxidant effect assays demonstrate that folic acid-LDH antioxidant systems have an obvious DPPH scavenging effect, hydroxyl radicals scavenging effect, and pro-oxidative Cu2+ chelating effect. The in vitro cytotoxicity assays indicate that the folic acid-LDH antioxidant system has no significant cytotoxic effect or obvious toxicity to normal cells. The in vivo antifatigue study shows that folic acid-LDH can prolong the forced swimming time of the mice. Folic acid-LDH nanomaterials are more effective in decreasing BUN and BLA, and increasing muscle and hepatic glycogen levels compared to folic acid groups and control groups. These results imply that the folic acid-LDH antioxidant system increases the endurance capacity and facilitates recovery from fatigue. They indicate that it may be used as a novel antioxidant or antifatigue nutritional supplement. Future work will focus on the study of antioxidant and antifatigue mechanisms at the molecular level.


This work was financially supported by the National Natural Science Foundation of China (Grant No 31100855, 31260251, 81301310), a Special Financial Grant from the China Postdoctoral Science Foundation (Grant No 2012T50440).


The authors report no conflicts of interest in this work.



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