Impact of RNA integrity and blood sample storage conditions on the gene expression analysis
Received 4 December 2017
Accepted for publication 16 April 2018
Published 20 June 2018 Volume 2018:11 Pages 3573—3581
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 3
Editor who approved publication: Prof. Dr. Jianmin Xu
Yanting Shen,1,2 Rui Li,1 Fei Tian,1 Zhenzhu Chen,1 Na Lu,2 Yunfei Bai,2 Qinyu Ge,2 Zuhong Lu1,2
1School of Biomedical Engineering, Southeast University, Nanjing 210096, Jiangsu Province, People’s Republic of China; 2State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, Jiangsu Province, People’s Republic of China
Background: The reliability of RNA sequencing (RNA-seq) output is affected by the quality of RNAs, which is in turn dependent on the quality of samples. Therefore, the purposes of this study were to reconsider the threshold of the RNA integrity number (RIN) and propose a simple and efficient storage scheme of blood samples for RNA-seq.
Patients and methods: The RNAs were extracted from blood samples that were stored at different conditions and used for sequencing. The bioinformatic analyses were performed to evaluate the impact of RNA integrity and blood sample storage conditions on the gene expression analysis.
Results: Our outcomes showed that the samples with RIN values more than 5.3 scarcely affected the quantitative results of RNA-seq, and the influence of inherent cellular physiological processes on RNA-seq output could be negligible.
Conclusion: The blood samples stored at 4°C within 7 days with RIN values more than 5.3 were available for RNA-seq.
Keywords: RNA, NGS, blood, integrity, RIN, sequencing
This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.Download Article [PDF] View Full Text [HTML][Machine readable]