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Human Monocytes/Macrophage Inflammatory Cytokine Changes Following in vivo and in vitro Schistomam manoni Infection

Authors Wolde M, Laan LC, Medhin G, Gadissa E, Berhe N, Tsegaye A

Received 4 October 2019

Accepted for publication 6 December 2019

Published 16 January 2020 Volume 2020:13 Pages 35—43

DOI https://doi.org/10.2147/JIR.S233381

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Ning Quan


Video abstract presented by Mistire Wolde.

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Mistire Wolde, 1, 2 Lisa C Laan, 3 Girmay Medhin, 1 Endalemaw Gadissa, 4 Nega Berhe, 1, 5 Aster Tsegaye 2

1Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia; 2Department of Medical Laboratory Sciences, College of Health Science, Addis Ababa University, Addis Ababa, Ethiopia; 3Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, the Netherlands; 4Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia; 5Oslo University Hospital-Ulleval, Centre for Imported and Tropical Diseases, Oslo, Norway

Correspondence: Mistire Wolde
Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia
Email mistire08@gmail.com

Introduction: Epidemiological and animal studies indicate that helminth infections have positive effects due to their potential to protect against autoimmune diseases. Here, we aim to assess the effect of S. mansoni infection on immune modulation of human monocytes and their potential protection against autoimmune disease development both in vivo and in vitro.
Materials and Methods: Monocytes were isolated from helminth-infected Ethiopians (MHIE), and from Dutch healthy volunteers (MHV). The MHV were stimulated in vitro with S. mansoni soluble egg antigens (SEA) or soluble worm antigens (SWA). In addition, phenotypical changes were studied directly, as well as after culturing for 6 days in the presence of human serum to obtain macrophages. Q-PCR, flow cytometry, multiplex bead immunoassay, and live-cell imaging were employed during analysis.
Results: MHIE showed elevated transcripts of SOCS-1 and TNF-α compared to MHV. Similarly, MHV that were stimulated with SEA demonstrated enhanced levels of SOCS-1, IL-10, and IL-12 mRNA, compared to control MHV. Remarkably, the SEA-treated monocytes showed a much higher motility than control monocytes, a hallmark of a patrolling phenotype. Furthermore, in vitro cultured macrophages that were stimulated by SEA exhibited enhanced mRNA levels of SOCS-1, IL-10, TNF-α, IL-12 and TGF-β, compared to control macrophages.
Conclusion: Macrophages from MHIE as well as SEA-treated MHV show an intermediate activation phenotype with both pro-inflammatory and anti-inflammatory characteristics in vitro. The observed pro-inflammatory properties might reflect a recent response of the cells due to contact with a pathogen, whereas the anti-inflammatory properties might contribute to helminth-induced protection against inflammatory diseases. Large-scale study is recommended to consolidate the findings of the present study.

Keywords: Schistosoma mansoni, immune modulation, monocytes, macrophages

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