Human gastric biopsy-derived lactobacilli suppress Helicobacter pylori-induced interleukin-8 production from gastric epithelial cells in vitro
Wimonrat Panpetch1, Thien Thiraworawong1, Somying Tumwasorn2
1Interdisciplinary Program of Medical Microbiology, Graduate School, Chulalongkorn University, Bangkok, Thailand; 2Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
Abstract: In this report we evaluate the anti-inflammatory effect of a variety of Lactobacillus spp. isolated from human gastric biopsies of dyspeptic patients. These lactobacilli were previously shown to secrete factors that inhibit tumor necrosis factor production from human monocytoid cells in vitro. Conditioned media from these lactobacilli were tested for the ability to modulate Helicobacter pylori-induced interleukin-8 (IL-8) production in AGS gastric epithelial cells. Out of 26 Lactobacillus spp. isolated from urease-negative patients thought not to harbor H. pylori, five significantly inhibited IL-8 production. The IL-8-inhibitory lactobacilli were identified as L. casei or L. paracasei, L. plantarum, L. rhamnosus, and L. salivarius and had no antagonistic effects on H. pylori growth. Our findings suggest potential candidates of Lactobacillus for further investigation of their beneficial effects on H. pylori-infected patients.
Keywords: Lactobacillus, Helicobacter pylori, interleukin-8, stomach, gastric biopsy, probiotics
© 2011 The Author(s). This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.