Back to Journals » International Journal of Nanomedicine » Volume 7

Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury

Authors Vaněček V, Zablotskii, Forostyak, Růžička, Herynek V, Babic M, Jendelova P, Kubinová S, Dejneka, Syková E

Received 11 April 2012

Accepted for publication 20 May 2012

Published 16 July 2012 Volume 2012:7 Pages 3719—3730


Review by Single anonymous peer review

Peer reviewer comments 3

Václav Vaněček,1,5 Vitalii Zablotskii,2 Serhiy Forostyak,1,5 Jiří Růžička,1 Vít Herynek,3 Michal Babič,4 Pavla Jendelová,1,5 Šárka Kubinová,1 Alexandr Dejneka,2 Eva Syková1,5

1Institute of Experimental Medicine AS CR, Prague, Czech Republic; 2Institute of Physics AS CR, Prague, Czech Republic; 3MR-Unit, Institute for Clinical and Experimental Medicine, Prague, Czech Republic; 4Institute of Macromolecular Chemistry AS CR, Prague, Czech Republic; 5Department of Neuroscience, 2nd Faculty of Medicine, Charles University, Prague, Czech Republic

Abstract: The transplantation of mesenchymal stem cells (MSC) is currently under study as a therapeutic approach for spinal cord injury, and the number of transplanted cells that reach the lesioned tissue is one of the critical parameters. In this study, intrathecally transplanted cells labeled with superparamagnetic iron oxide nanoparticles were guided by a magnetic field and successfully targeted near the lesion site in the rat spinal cord. Magnetic resonance imaging and histological analysis revealed significant differences in cell numbers and cell distribution near the lesion site under the magnet in comparison to control groups. The cell distribution correlated well with the calculated distribution of magnetic forces exerted on the transplanted cells in the subarachnoid space and lesion site. The kinetics of the cells’ accumulation near the lesion site is described within the framework of a mathematical model that reveals those parameters critical for cell targeting and suggests ways to enhance the efficiency of magnetic cell delivery. In particular, we show that the targeting efficiency can be increased by using magnets that produce spatially modulated stray fields. Such magnetic systems with tunable geometric parameters may provide the additional level of control needed to enhance the efficiency of stem cell delivery in spinal cord injury.

Keywords: magnetism, mesenchymal stem cell, nanoparticle, spinal cord injury, modeling

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]