Back to Journals » Journal of Inflammation Research » Volume 13

High-Intensity Interval Training Reversed High-Fat Diet–Induced M1-Macrophage Polarization in Rat Adipose Tissue via Inhibition of NOTCH Signaling

Authors Shanaki M, Khosravi M, Khoshdooni-Farahani A, Dadashi A, Heydari MF, Delfan M, Jafary H, Gorgani-Firuzjaee S

Received 2 November 2019

Accepted for publication 22 January 2020

Published 17 March 2020 Volume 2020:13 Pages 165—174


Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Ning Quan

Mehrnoosh Shanaki, 1 Maryam Khosravi, 2 Arezoo Khoshdooni-Farahani, 3 Alireza Dadashi, 4 Mohammad Foad Heydari, 5 Maryam Delfan, 6 Hanieh Jafary, 3 Sattar Gorgani-Firuzjaee 5

1Department of Medical Laboratory Sciences, School of Allied Health Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; 3Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran; 4Department of Infectious Disease, School of Medicine, AJA University of Medical Sciences, Tehran, Iran; 5Department of Medical Laboratory Sciences, School of Allied Health Medicine, AJA University of Medical Sciences, Tehran, Iran; 6Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Alzahra University, Tehran, Iran

Correspondence: Sattar Gorgani-Firuzjaee

Introduction: There is accumulating evidence on the beneficial effect of exercise intervention in the management of metabolic disorders; however, the molecular mechanism is still unclear. Here, the current study aimed to compare the effect of high-intensity interval training (HIIT) and continuous endurance training (CET) on serum and adipose-tissue markers of M1/M2 macrophage polarization.
Methods: A total of  45 healthy male Wistar rats were divided into groups of normal chow (n=10) and high-fat diet (HFD) (n=35). Then, rats receiving the HFD were randomly divided into four groups. Training programs were performed for 5 days/week over 10 weeks. The CET protocol included 30 minutes running at 50%– 60% of VO 2max. The HIIT protocol consisted of five repeated intervals of 2-minute sprints on the treadmill at 80%– 90% VO 2max workload with 1 minute’s 30%– 35% VO 2max interval for each rat. Then, biochemical parameters were assessed. Macrophage-polarization markers were assessed at mRNA and protein levels by real-time PCR and Western blotting, respectively.
Results: Both exercise-training programs, especially HIIT, reversed increased serum biochemical parameters (glucose, triglycerides, cholesterol, Homeostatic Model Assessment of Insulin Resistance, and hsCRP), M1-polarization markers (circulating IL6, TNFα, and adipose-tissue mRNA expression of IL6, TNFα and iNOS), M2 markers (CD206, CD163, and IL10 expression), as well as pIκKB, pNFκB, and NICD expression in HFD-induced diabetes.
Conclusion: Our findings suggest that despite devoting less time, the HIIT workout is a more effective intervention for diabetes management. Moreover, HIIT reverses HFD-induced macrophage polarization by targeting the NFκB and NOTCH signaling pathways.

Keywords: obesity, diabetes, macrophage polarization, high-intensity interval training, continuous endurance training

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]