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Gold nanorod–based poly(lactic-co-glycolic acid) with manganese dioxide core–shell structured multifunctional nanoplatform for cancer theranostic applications

Authors Wang L, Li D, Hao Y, Niu M, Hu Y, Zhao H, Chang J, Zhang Z, Zhang Y

Received 28 November 2016

Accepted for publication 24 February 2017

Published 13 April 2017 Volume 2017:12 Pages 3059—3075


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 6

Editor who approved publication: Dr Lei Yang

Lei Wang,1–3 Dong Li,1,2 Yongwei Hao,1,2 Mengya Niu,1,2 Yujie Hu,1,2 Hongjuan Zhao,1,2 Junbiao Chang,2,3 Zhenzhong Zhang,1,2 Yun Zhang1,2

1School of Pharmaceutical Sciences, Zhengzhou University, 2Key Laboratory of Targeting Therapy and Diagnosis for Critical Disease, Henan Province, 3School of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, People’s Republic of China

Abstract: Recently, photothermal therapy has become a promising strategy in tumor treatment. However, the therapeutic effect was seriously hampered by the low tissue penetration of laser. Therefore, in this study, radiofrequency (RF) with better tissue penetration was used for tumor hyperthermia. First, one type of gold nanorods (AuNRs) suitable for RF hyperthermia was selected. Then, poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) loaded with AuNRs and docetaxel (DTX) (PLGA/AuNR/DTX) NPs were constructed. Finally, manganese dioxide (MnO2) ultrathin nanofilms were coated on the surfaces of PLGA/AuNR/DTX NPs by the reduction of KMnO4 to construct the PLGA/AuNR/DTX@MnO2 drug delivery system. This drug delivery system can not only be used for the combined therapy of chemotherapy and RF hyperthermia but can also produce Mn2+ to enable magnetic resonance imaging. Furthermore, the RF hyperthermia and the degradation of MnO2 can significantly promote the controlled drug release in a tumor region. The in vitro and in vivo results suggested that the PLGA/AuNR/DTX@MnO2 multifunctional drug delivery system is a promising nanoplatform for effective cancer theranostic applications.

poly(lactic-co-glycolic acid), gold nanorod, manganese dioxide, radiofrequency, hyperthermia, dual-mode imaging, controlled release


Cancer has a serious threat to the health and life of human beings. Currently, the main measures of treatment include chemotherapy, surgical treatment, and radiation therapy.1,2 However, there are many disadvantages in these therapies, which lead to the nonideal therapeutic effect.3 With the development of tumor adjunctive therapy, the combined therapy of hyperthermia and chemotherapy has become a hot spot in related research fields.4,5 Gold nanomaterials are a type of biomaterials that exhibit many excellent properties, such as easy molding, easy modification, and low toxicity, and they have always been used for near-infrared (NIR) light-induced photothermal therapy (PTT).6,7 However, low tissue penetration and skin burning of NIR laser have seriously hindered its use.8

Using radiofrequency (RF) is a handy and effective method to solve the abovementioned problems. Moreover, at present, RF hyperthermia has been used as an auxiliary clinical treatment for cancer.9,10 It brings about several advantages for cancer therapy, including simple operation, no wound, and full-body penetration. However, long-time RF hyperthermia is also harmful to a person’s health due to the electromagnetic wave emitted by an RF. Shortening the treatment time, reducing the treatment damage, and improving the treatment effect are urgently needed. Therefore, finding an effective sensitizer of RF hyperthermia is the key. Gold nanorods (AuNRs) are rod-like gold nanomaterials that are always used for light-induced photothermal (photodynamic) therapy.11 Song et al used AuNRs for doxorubicin loading and PTT.12 Wang et al reported that the modified AuNRs had unique optical and photothermal properties, making them promising nanostructures for biomedical applications.13 Recently, Collins et al reported that gold nanoparticles (NPs) can be used for RF-induced hyperthermia.14 Rejinold et al summarized many nanomaterials (including gold nanomaterials), which can be used for RF hyperthermia in vitro.15 However, the present study found that AuNRs with different aspect ratios (ARs) have different heating efficiencies induced by RF; therefore, in this study, AuNRs with a suitable AR were selected for RF-induced hyperthermia in vitro and in vivo.

It was reported that cetyltrimethylammonium bromide (CTAB) used for the construction of AuNRs always causes obvious toxicity to the normal cells.16 Moreover, as a commonly used anti-tumor chemotherapeutic drug in the clinical application, docetaxel (DTX) is restricted due to its high hydrophobicity and severe side effects.17,18 Therefore, a suitable delivery system should be prepared to reduce the toxicity of CTAB and DTX to the normal cells and tissues. Poly(lactic-co-glycolic acid) (PLGA) NPs are a family of US Food and Drug Administration–approved biodegradable polymers that are physically strong and highly biocompatible and have been extensively studied as delivery vehicles for drugs, proteins, and various other macromolecules.19 Thus, PLGA NPs were selected for loading AuNRs and DTX, and should be able to provide chemotherapy and RF hyperthermia at the same time.

Compared with the normal tissues, tumor microenvironment has unique characteristics, such as lower pH value and higher glutathione (GSH) level.20,21 In a previous study, a type of multifunctional nanosheet based on manganese dioxide (MnO2) was prepared, and it was shown that MnO2 nanosheets could respond to a slightly acidic environment and high concentration of reduced GSH, which caused the degradation of MnO2 into manganese ions enabling T1-weighted magnetic resonance imaging (MRI).22 Decorating MnO2 sheets on the surface of PLGA could bring the MRI function and controlled release function to the nanoplatform in the tumor sites. There are some studies on the combination therapy of GSH-sensitive controlled release and PTT. Han et al synthesized a novel conjugate by conjugating paclitaxel to paclitaxel by using disulfide linkage. The conjugate could self-assemble into uniform NPs with 1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide encapsulated for PTT, which showed high antitumor effect.23 Hou et al used –s–s– to construct a GSH-sensitive controlled release drug delivery system for the combined PTT, by using the carbon nanotubes.24 It was reported that the –s–s– bond used in many nano drug delivery systems was always used for redox–responsive releasing, and some of these systems can be used for combined PTT, indicating that the combined therapy of redox–responsive releasing and hyperthermia is an effective method for tumor inhibition.25,26 The aim of the present study was to prepare a new kind of core–shell drug delivery system (PLGA/AuNR/DTX@MnO2). As illustrated in Figure 1, the AuNRs with a suitable AR and DTX were loaded in PLGA NPs by using the emulsion solvent evaporation method. Then, MnO2 ultrathin nanosheets were loaded on the surface of PLGA to obtain PLGA/AuNR/DTX@MnO2 drug delivery system. The efficiency of the multifunctional nanoplatform was assessed in vitro and in vivo, and the results demonstrated the combined therapeutic effect of RF hyperthermia and chemotherapy, the controlled release effect, and an MRI effect in cancer theranostic applications.

Figure 1 Scheme of PLGA/AuNR/DTX@MnO2 and its biofunctions.
Abbreviations: AuNR, gold nanorod; CT, computed tomography; DTX, docetaxel; GSH, glutathione; KMnO4, potassium permanganate; MES, 2-(N-morpholino) ethanesulfonic acid; MRI, magnetic resonance imaging; MnO2, manganese dioxide; PLGA, poly(lactic-co-glycolic acid); RF, radiofrequency.

Materials and methods


PLGA (lactide/glycolide =50:50, molecular weight =94,000 Da) was purchased from Daigang Biomaterial Co., Ltd. (Jinan, People’s Republic of China). Chloroauric acid (HAuCl4) was obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, People’s Republic of China). CTAB was obtained from Dibo Ltd. (Shanghai, People’s Republic of China). Ascorbic acid (AA), sodium borohydride (NaBH4), and silver nitrate (AgNO3) were purchased from Guobang Pharmaceutical Company (Shanxi, People’s Republic of China). GSH (purity >98%) was obtained from Genview Co., Ltd. (Beijing, People’s Republic of China). DTX was obtained from Meilun Biotechnological Company (Dalian, People’s Republic of China). Poly(vinyl alcohol) (PVA) was obtained from Kelong Chemical Reagent Company (Chengdu, People’s Republic of China). Both potassium permanganate (KMnO4) and 2-(N-morpholino) ethanesulfonic acid (MES) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, People’s Republic of China). The Michigan Cancer Foundation – 7 (MCF-7) human breast cancer cell line was purchased commercially from the Cell Bank of the Chinese Academy of Sciences (Catalog No TCHu 74; Shanghai, People’s Republic of China). All the other chemicals were obtained from Sigma-Aldrich Co. Ltd (St Louis, MO, USA).

Preparation of the gold seeds solution

First, 0.5 mL of HAuCl4 (0.5 mM) was added drop by drop to 5 mL of CTAB solution (0.2 M) under magnetic stirring with a rate of 550 cycles/min at an ambient temperature. Second, chilled NaBH4 (0.6 mL, 0.01 M) was added to the abovementioned mixture solution. When the color of the mixing solution changed from pale yellow to claybank, the seed solution was stirred vigorously for 2 min and kept at room temperature for 30 min away from light for further use.

Preparation of the growth solution

First, 8.0 mL of HAuCl4 (0.5 mM) was mixed with 5.0 mL CTAB (0.20 M) under slight stirring, and then, different volumes of 0.004 M AgNO3 solution (20, 40, 100, 280, and 360 μL) were added to the mixture at room temperature. Then, 140 μL AA of 0.0788 M was added drop by drop into the five samples. Following the addition of AA, the colors of the growth solutions changed from dark yellow, and the solutions became colorless.

Preparation of AuNRs

According to the CTAB-induced seed-mediated growth method,27,28 the AuNRs (AR =1.48:1, 2.34:1, 3.14:1, 3.52:1, and 4.20:1) were synthesized.

First,1 mL of gold seed solution was added into the growth solution and stirred at 350 cycles/min. The colors of the solutions gradually changed within ~10–20 min. The mixture was kept for 1 h at room temperature, and the AuNRs products were obtained after centrifugation (12,000 rpm; 15 min) and rinsing three times to remove the excess CTAB.

In order to select the most suitable AR of AuNRs for RF hyperthermia, the Au concentration of each AuNRs sample was set at 100 μg/mL. Subsequently, the samples were exposed to an RF field (13.56 MHz; 400 W) for 5 min, and the temperatures were recorded. Finally, the AuNRs sample with the best heat production was chosen for further experiments.

Construction of PLGA/AuNR/DTX

The PLGA/AuNR/DTX NPs were constructed by using the emulsion solvent evaporation method reported in the previous studies.29,30 In order to prepare the organic phase, PLGA (100 mg) and DTX (6 mg) were dissolved into acetone (5 mL). Furthermore, 5 mL of AuNRs (2 mg/mL) were dissolved in 10 mg/mL PVA (20 mL) to obtain the aqueous phase. Then, the organic phase was added drop by drop into the aqueous phase in a magnetic stirrer with a rate of 550 rpm at room temperature for ~4 h for complete acetone evaporation. Finally, PLGA/AuNR/DTX NPs were collected by centrifugation (15,000 g) for 30 min and washed four times with deionized water.

Preparation of PLGA/AuNR/DTX@MnO2

MnO2 nanosheets were loaded on the surface of the PLGA/AuNR/DTX NPs by using the MES oxidation–reduction method.31 Briefly, the above PLGA/AuNR/DTX NPs were added into 6.25 mL MES buffer (100×10−3 M; pH =6.0) under ultrasonic conditions, and then, KMnO4 solution (5 mM) was added and mixed for ~5 min. The above mixture was subjected to dialysis for 12 h to remove the by-products. After centrifugation, PLGA/AuNR/DTX@MnO2 NPs were collected and concentrated by freeze-drying for further use.


Small drops of AuNRs with different ARs, PLGA/DTX, PLGA/AuNR/DTX, and PLGA/AuNR/DTX@MnO2 solutions were placed on the grids. Transmission electron microscopy (TEM; JEM-1200 EX, 120 kV) and cryogenic TEM (JEOL 2100 HC; Tokyo, Japan) were used to observe the morphologies of the solutions. The energy-dispersive X-ray (EDX) spectroscopy was also tested to analyze the chemical elements of the NPs. The optical properties of NPs were characterized by using an ultraviolet–visible (UV-Vis) spectrometer (UV-Vis 2700; Shimadzu Co. Ltd., Kyoto, Japan). The temperatures of AuNRs (Au concentration =100 μg/mL) exposed to RF were monitored by using an Infrared Thermometer (IR 97; HuaShengChang Instrument Co. Ltd.; Shenzhen, People’s Republic of China). X-ray photoelectron spectroscopy (XPS) was used to analyze the chemical properties of Mn. XPS data were processed with XPSPEAK software, Version 4.1. The size distribution and zeta potential of the NPs with or without 10% fetal bovine serum were measured by using dynamic light scattering (DLS; ZS-90; Malvern Instruments, Worcestershire, UK). DLS was also used to determine the stability of PLGA/AuNR/DTX@MnO2 NPs in 10% fetal bovine serum.

The PLGA/AuNR/DTX@MnO2 NPs were dissolved in phosphate-buffered saline (PBS; pH =7.4) containing 0 mM and 2 mM GSH. Then, 2 mL of this solution was separately dropped into a 24-well cell culture plate. After 4 h, in vitro T1-weighted MR images were obtained with a 1.5-T clinical MR scanner (Signa Excite; GE Healthcare Systems, Milwaukee, WI, USA). A MicroMR instrument (0.5 T; Shanghai Niumag Electronic Technology Co., Ltd., Shanghai, People’s Republic of China) was used for the analysis of the longitudinal (T1) relaxivity values.

Determination of loading and release rates

The MnO2 shells of PLGA/AuNR/DTX@MnO2 NPs were first digested by adding dithiothreitol. The encapsulation ratio of DTX was determined according to the method reported by a prior research.32 First, DTX was extracted from PLGA/AuNR/DTX NPs by adding the digested NPs into the mixture of methanol and acetonitrile (v/v =1:3). After sonication for 30 min, the suspension was centrifuged, and the collected supernatant was measured by using high performance liquid chromatograph (HPLC, Agilent 1200, Agilent Technologies Inc., Santa Clara, CA, USA) in order to detect the concentration of DTX. A reverse-phase Intertex C18 column (Sciencehome, 5 μm, 4.6×250 mm) was used as an analytical column, and the flow rate of mobile phase was set at 1 mL/min. The mobile phase consisting of deionized water and methanol (v/v =27:73) was applied. DTX eluting from the column was detected by using a UV detector set at 231 nm. The encapsulation ratio was calculated. Besides this, the MnO2 and AuNR contents were measured by using inductively coupled plasma–atomic emission spectrometry (ICP-AES).

For the in vitro release study, PLGA/AuNR/DTX and PLGA/AuNR/DTX@MnO2 samples were placed separately into dialysis bags (cutoff mass =12,000 Da), which were dialyzed in 35 mL PBS (0.1 M; pH =7.4 or 5.0) containing 0.5% sodium dodecyl sulfonate at 37°C with a stirring rate of 100 rpm. Both the samples were divided into two groups: experimental group and control group. The PBS in the control group contained 2 mM GSH, and the other did not contain GSH. During the experimental process, 0.5 mL of solution was drawn from the solution outside the dialysis bags at 15 and 30 min and 1, 2, 4, 6, 8, 12, 24, 36, 48, and 72 h. Meanwhile, the same volume of the release medium solution was replenished. The concentration of DTX released from NPs into release medium was quantified by using an HPLC under the above chromatographic conditions.

Cellular experiments

Cell culture

The MCF-7 human breast cancer cell line was cultured in Dulbecco’s Modified Eagle’s Medium with 1% penicillin/streptomycin and 10% fetal bovine serum at 37°C in a humidified incubator.

Cellular uptake

In order to determine the cellular uptake ratio of PLGA/AuNR/DTX@MnO2, fluorescein isothiocyanate (FITC) was used for fluorescence tracing. The FITC-labeled NPs were prepared by using the abovementioned steps (in “Construction of PLGA/AuNR/DTX” and “Preparation of PLGA/AuNR/DTX@MnO2” sections). FITC was added in the organic phase in each group with the final concentration of 0.125 μg/mL. The cells were cultured on the 6-well plates for 24 h and then exposed to FITC, PLGA/AuNR/DTX, and PLGA/AuNR/DTX@MnO2 NP solution. After incubation for 5 h, the old medium of different samples was removed and the cells were washed with PBS for twice. Finally, the cells were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; 1.2 μg/mL) for 20 min and then imaged with a laser confocal microscope (Fluoview V1000, Olympus Optical Co., Ltd., Tokyo, Japan). For further evaluating the cellular uptake of the NPs, the above samples were analysed by using flow cytometry (FCM; Epics XL MCL™) three times.

Cell viability

MCF-7 cells with a density of ~8.0×103 per well were inoculated in 96-well plates and cultivated for 24 h. Subsequently, the culture medium was replaced with different concentrations of formulations diluted in fresh culture medium (DTX concentration =0.5, 1, and 2 μg/mL; Au concentration =10, 20, and 40 μg/mL). For the RF groups, the cells were exposed to a 13.56-MHz RF field for 5 min, and then they were incubated with the preparations for 5 h. Cell inhibition was measured by using sulforhodamine B assay after a further 24 h of incubation.33 The cells were divided into the following groups: DTX, PLGA/AuNR/MnO2, PLGA/DTX@MnO2, and PLGA/AuNR/DTX@MnO2 groups with or without RF treatment.

Cell apoptosis and GSH level

MCF-7 cells with a density of ~8.0×103 per well were inoculated in 96-well plates and cultivated for 24 h. Then, the cells were incubated with DTX, PLGA/DTX, PLGA/AuNR, PLGA/AuNR/DTX, and PLGA/AuNR/DTX@MnO2 (DTX concentration =2 μg/mL; Au concentration =40 μg/mL) with or without RF treatment for 5 h and washed with PBS, and then, fresh medium was added. The treatment of RF groups was the same as described in the “Cell viability” section. The cells were cultured for another 24 h, and then they were resuspended in 500 μL of Annexin V-FITC binding buffer, stained with 5 μL Annexin V-FITC and 5 μL propidium iodide (PI) for 15 min in a dark environment, and finally analyzed by using a flow cytometer (Accuri C6, BD Co. Ltd., San Jose, CA, USA). In addition, the GSH change after the treatment of PLGA/AuNR/DTX@MnO2 was detected by using the reduced GSH assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, People’s Republic of China).

Animal experiments

The animal studies were conducted on female Kunming mice (~18–20 g), which were purchased from Henan Laboratory Animal Center. All the mice received care in compliance with the criteria of the National Regulation on the Management of Laboratory Animals. A subcutaneous S-180 tumor model was established, and when the tumor volume reached ~80–100 mm3 (~7 days after tumor inoculation), the treatment was started. This study was approved by the Ethics Committee of Zhengzhou University.

Tumor growth inhibition

The mice were divided into the following groups (five mice per group): saline, DTX, PLGA/DTX, PLGA/AuNR, PLGA/AuNR/DTX, and PLGA/AuNR/DTX@MnO2 (DTX dosage =15 mg/kg) with or without RF irradiation treatment. The preparations were intravenously injected into the tumor-bearing mice every other day. In addition, the body weights and the tumor sizes (the tumor volume =0.5× [tumor length] × [tumor width]2) were measured every other day. The RF treatment was performed by exposing the tumor site to the RF field (RF condition: 13.56 MHz; 400 W; 5 min) at 4 h after injection. After treatment for five times, the mice were sacrificed to collect the tumors for hematoxylin and eosin (H&E) staining. Simultaneously, the tumors of each group were photographed and measured for cell apoptosis by using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method. The morphological changes were observed under a fluorescence microscope (Eclipse 50; Nikon Co. Ltd., Tokyo, Japan).

Biodistribution of PLGA/AuNR/DTX@MnO2

The tumor-bearing mice were given DTX or PLGA/AuNR/DTX@MnO2 (DTX dose =15 mg/kg), via a tail vein injection. After treatment for 1, 2, 4, 6, and 8 h, the tissues/organs were collected and homogenized in buffer (acetonitrile to saline ratio =1:1). DTX in tissues/organs was determined by using an HPLC (Agilent 1100, USA) under the following conditions: an Eclipse XDB-C18 column (150×4.6×5.0 mm), mobile-phase acetonitrile/water =50:50, column temperature =30°C, detection wavelength =231 nm, flow rate =1.0 mL/min, and injection volume =20 mL.

MRI and X-ray imaging

In order to determine the diagnostic potential of PLGA/AuNR/DTX@MnO2 nanoplatform, MRI and X-ray imaging properties were detected. For in vivo imaging, PLGA/AuNR/DTX@MnO2 NPs (200 μL; DTX concentration =15 mg/kg) were intravenously injected into the tumor-bearing mice. After injection of PLGA/AuNR/DTX@MnO2, MRI and X-ray imaging were conducted on a clinical 3-T MRI scanner and X-ray scanner, respectively.

Statistical analysis

Quantitative data were expressed as mean ± standard deviation and analyzed by using the analysis of variance followed by Dunnett’s posttest. P-values <0.05 were considered statistically significant.

Results and discussion

Characterization of AuNRs

In recent years, due to excellent physical and chemical properties, AuNRs have attracted great attention.34 AuNRs with different ARs have different absorption wavelengths that can be used for a variety of purposes, such as PTT.35,36 However, because of some drawbacks of NIR PTT, the RF irradiation has been used in this study for AuNRs-based hyperthermia.

The TEM images (Figure 2A) indicate that AuNRs with different ARs (1.5:1, 2.3:1, 3.1:1, 3.5:1, and 4.2:1) were synthesized successfully. Meanwhile, UV-Vis spectroscopic analysis proved that their localized surface plasmon resonance (LSPR) peak extends from ~520 to ~840 nm in the NIR region (Figure 2B). It was reported that the electromagnetic wave can easily penetrate the sharp tip structure and participate in heat production.37 Furthermore, a previous study showed that, as a kind of electromagnetic wave, RF can induce LSPR for heat production by using gold nanostars.38 As shown in Figure 2C, the temperatures increased with an increase in the ARs of AuNRs, demonstrating that AuNRs can be used as an RF-induced thermal-sensitive agent, and the AuNRs with the AR of 4.2:1 were selected for further research.

Figure 2 Characterization of AuNRs.
Notes: (A) TEM images of AuNRs with different ARs; (B) UV-Vis spectra of AuNRs with different ARs; (C) the temperatures (after RF irradiation) of AuNRs with different ARs. (a) 1.5:1; (b) 2.3:1; (c) 3.1:1; (d) 3.5:1; (e) 4.2:1.
Abbreviations: ARs, aspect ratios; AuNRs, gold nanorods; RF, radiofrequency; TEM, transmission electron microscopy; UV-Vis, ultraviolet–visible.

Characterization of PLGA/AuNR/DTX@MnO2

PLGA/AuNR/DTX@MnO2 enclosing DTX and AuNRs were prepared by the emulsion solvent evaporation method, followed by coating with MnO2 nanosheets and through the reduction reaction (Figure 1). PVA was used in the process of construction of PLGA/AuNR/DTX NPs, which can maintain the stability of NPs. Furthermore, the hydroxyl groups of PVA could be used for the construction of MnO2 on the PLGA/AuNR/DTX NPs through the reduction of KMnO4 by MES.39 The morphologies of PLGA/DTX, PLGA/AuNR/DTX, and PLGA/AuNR/DTX@MnO2 were determined by using TEM (Figure 3A). The results showed that all the three NPs showed clearly a spherical shape, and the AuNRs were wrapped in the PLGA NPs (Figure 3A-b). Then, the core–shell structure was constructed. The PLGA/AuNR/DTX NPs acted as the inner core, and the MnO2 nanosheets acted as the shell. It can be seen that after MnO2 nanosheets were coated on the surface of the PLGA/AuNR/DTX, the smooth surface of the NPs had become rough. In the whole process, the above three samples showed different colors (Figure 3B-a, b, and c).

Figure 3 Characterization of PLGA/AuNRs/DTX@MnO2.
Notes: (A) TEM images of the NPs: (a) PLGA/DTX, (b) PLGA/AuNR/DTX, and (c) PLGA/AuNR/DTX@MnO2; (B) Photographs of the NPs: (a) PLGA/DTX, (b) PLGA/AuNR/DTX, and (c) PLGA/AuNR/DTX@MnO2; the particle size of the NPs; (d) PLGA/DTX, (e) PLGA/AuNR/DTX, and (f) PLGA/AuNR/DTX@MnO2; (C) UV-Vis spectra of DTX, PLGA/DTX, PLGA/AuNR/DTX, and PLGA/AuNR/DTX@MnO2; (D) XPS curve of the PLGA/AuNR/DTX@MnO2 NPs.
Abbreviations: AuNR, gold nanorod; DTX, docetaxel; MnO2, manganese dioxide; NPs, nanoparticles; PLGA, poly(lactic-co-glycolic acid); TEM, transmission electron microscopy; UV-Vis, ultraviolet–visible; XPS, X-ray photoelectron spectroscopy.

The colors of PLGA/DTX, PLGA/AuNR/DTX, and PLGA/AuNR/DTX@MnO2 were milky white, pink, and brown, respectively. Their average sizes were 239.0±3.7, 263.3±4.5, and 282.1±6.2 nm, respectively (Figure 3B-d, e and f), and the polymer dispersion index (PDI) values of them were 0.16±0.01, 0.16±0.02, and 0.18±0.02, respectively. The zeta potential of the AuNRs was 27.4±2.2 mV, and this positive potential was attributed to CTAB that was coated on the surface of the AuNRs.40 However, the biotoxicity of CTAB and the low drug-loading rate of AuNRs are the obstacles for their use in the biomedical area. The PLGA NPs used in this study were not only used to decrease the toxicity of the AuNRs but also to increase the drug-loading rate. When the AuNRs and DTX were loaded in PLGA NPs, the zeta potential of PLGA/AuNR/DTX NPs was decreased to −12.3±1.5 mV, and after loading the MnO2 nanosheets, the zeta potential changed to −9.7±1.4 mV. Figure S1 shows the low magnification view of PLGA/AuNR/DTX@MnO2, indicating that these NPs were nonaggregated. Furthermore, the stability of PLGA/AuNR/DTX@MnO2 NPs in a culture medium containing 10% fetal bovine serum was also determined, for 24 h. Tables S1 and S2 indicate that the particle size and zeta potential were broadly unchanged in the physiologically mimicked environment.

The construction of MnO2 shell was proved by the UV-Vis spectrum and XPS detection. As in Figure 3C, after the AuNRs were loaded in the PLGA/DTX NPs, the new peak ~800 nm was obtained, which was attributed to the AuNRs. Compared with the PLGA/AuNR/DTX NPs, the MnO2 nanosheets showed a new wide peak between 300 and 400 nm. For further determination, the XPS results (Figure 3D) indicate that the Mn 2p 3/2 and 2 p 1/2 were found to be 640.7 and 652.5 eV, respectively. These results were consistent with the reported data for MnO2.32 After the MnO2 nanosheets were constructed, the EDX spectrum (Figure 4A) of the PLGA/AuNR/DTX@MnO2 showed that the signal corresponding to Mn appeared. This result combined with all the above results, shown in Figure 3, indicated that the PLGA/AuNR/DTX@MnO2 delivery system was constructed successfully. The encapsulation ratio of DTX can reach 74.4%, which may be due to its hydrophobic property. The results of ICP-AES determination indicated that the AuNRs and MnO2 content in the NPs were ~8.2% and 5.0% in weight.

Figure 4 Characterization and determination of the drug release of PLGA/AuNRs/DTX@MnO2.
Notes: (A) EDX analysis: (a) PLGA/AuNR/DTX and (b) PLGA/AuNR/DTX@MnO2; (B) The cumulative release profile in vitro: (a) PLGA/AuNR/DTX, (b) PLGA/AuNR/DTX@MnO2 +0 mM GSH (pH =7.4), (c) PLGA/AuNR/DTX@MnO2 +0 mM GSH (pH =5.0), (d) PLGA/AuNR/DTX@MnO2 +2 mM GSH (pH =7.4); and (e) PLGA/AuNR/DTX@MnO2 +0 mM GSH (pH =5.0). The values are expressed as mean ± standard deviation (n=3).
Abbreviations: AuNR, gold nanorod; DTX, docetaxel; EDX, energy-dispersive X-ray; GSH, glutathione; MnO2, manganese dioxide; PLGA, poly(lactic-co-glycolic acid).

Determination of the drug release

In order to investigate the GSH and pH-triggered drug release, PLGA/AuNR/DTX NPs and PLGA/AuNR/DTX@MnO2 NPs were detected in the releasing medium with or without 2 mM GSH for the imitation of the physiological environment at a pH of 7.4 or 5.0 in vitro (Figure 4B). It was found that PLGA/AuNR/DTX NPs showed a similar release property with or without GSH treatment, and they also showed similar release property at a pH of 7.4 or 5.0 environment (P>0.05). As shown in Figure 4B, the PLGA/AuNR/DTX NPs rapidly released the encapsulated DTX within 12 h, and the cumulative release rate was ~82.1%. However, the release rate of PLGA/AuNR/DTX@MnO2 NPs was only ~39.7%. When the GSH was added into the releasing medium, the release speed was increased obviously, and the release percentage of PLGA/AuNR/DTX@MnO2 was increased to ~78.1%. Moreover, PLGA/AuNR/DTX@MnO2 NPs have a pH-sensitive controlled release property. When the pH value decreased to 5.0, the release rates were increased, with or without GSH. These results demonstrated that the MnO2 nanosheets could prevent the drug release. Moreover, GSH and lower pH values can induce the drug release because of the MnO2 surface degradation. GSH level was higher and pH level was lower in the tumor region than in the normal tissues.21 Therefore, GSH/pH dual-responsive controlled release has great potential for this drug delivery system.

In vitro MRI and X-ray computed tomography (CT) imaging

The previous studies reported that MnO2 can release Mn2+ ions in the tumor microenvironment.41 Therefore, MnO2 nanomaterials can be used for MRI in vitro and in vivo.22,42 In addition, the previous studies also showed that the gold nanomaterials can be used as the X-ray CT imaging contrast agents.29,38 In this drug delivery nanoplatform, AuNRs and MnO2 provided the dual-mode imaging for precise diagnosis of tumor. T1-weighted MRI (Figure 5A) of PLGA/AuNR/DTX@MnO2 NP solutions revealed that the MRI contrast was dependent on the concentration of delivery system and the GSH level. When the concentration of the solution increased, the brightness of T1-weighted MR image was enhanced. The groups with GSH were brighter than the groups without GSH at the same concentration, revealing the capability of PLGA/AuNR/DTX@MnO2 to be used as an effective T1-weighted MRI contrast agent.

Figure 5 Dual-mode imaging in vitro.
Notes: (A) T1-weighted MR images of PLGA/AuNR/DTX@MnO2 solutions at different Mn concentrations; (B) T1 relaxation rate R1 (1/T1) against Mn concentration; (C) In vitro X-ray CT images of PLGA/AuNR/DTX@MnO2 suspended in PBS. The Au concentration (μg/mL) in each sample is provided at the top of the respective images; (D) CT attenuation (HU) plot of PLGA/AuNR/DTX@MnO2 at various concentrations.
Abbreviations: AuNR, gold nanorod; CT, computed tomography; DTX, docetaxel; GSH, glutathione; MnO2, manganese dioxide; MR, magnetic resonance; PBS, phosphate-buffered saline; PLGA, poly(lactic-co-glycolic acid).

Figure 5C shows that the intensity of CT signal continuously increased with an increase in the concentration. The Hounsfield units were detected, indicating that PLGA/AuNR/DTX@MnO2 had great potential to be used as a positive X-ray CT imaging contrast agent. The MRI and X-ray CT imaging results demonstrated that the PLGA/AuNR/DTX@MnO2 NPs can be used for dual-mode imaging application for accurate tumor diagnosis.

Cellular uptake

Whether the NPs enter into the cells is an important consideration for cancer therapy. Therefore, the cellular uptake of PLGA/AuNR/DTX and PLGA/AuNR/DTX@MnO2 nanoplatforms was determined. The NPs were labeled with FITC fluorescence dye, and were tracked for the colocalization of FITC signal (green), and the cell nuclei were stained with DAPI (blue).

The fluorescence images showed that the NPs can enter into cells within 5 h (Figure 6A). The cellular uptake ratios of FITC, PLGA/AuNR/DTX, and PLGA/AuNR/DTX@MnO2 NPs were 7.61±0.38, 99.7±0.14, and 98.5%±0.53%, respectively (Figure 6B). It was found that there was no significant difference in the cellular uptake of NPs (P>0.05), indicating that both of them can enter into the cells efficiently within 5 h.

Figure 6 Cellular uptake.
Notes: (A) The fluorescence images of MCF-7 cells at 5 h: (a) FITC group, (b) PLGA/AuNR/DTX group, and (c) PLGA/AuNR/DTX@MnO2 group. (B) cellular uptake results of (a) FITC, (b) PLGA/AuNR/DTX, and (c) PLGA/AuNR/DTX@MnO2 at 5 h.
Abbreviations: AuNR, gold nanorod; DAPI, 4′,6-diamidino-2-phenylindole; DTX, docetaxel; FITC, fluorescein isothiocyanate; MnO2, manganese dioxide; PLGA, poly(lactic-co-glycolic acid).

Cell viability

The combination of hyperthermia and chemotherapy is the efficient method for cancer treatment in clinical and laboratory research. Many anti-cancer drugs can be combined with hyperthermia treatment. The blood supply in tumor peripheral regions is generally hypervascular, and can be delivered easily. However, in the central area of the tumor, the scanty blood inflow causes difficulty in drug delivery, cell hypoxia, and poor heat dissipation (heat is accumulated easily). Moreover, hypoxic cells are sensitive to hyperthermia. Therefore, hyperthermia and chemotherapy can complement each other and can be combined to enhance the anti-tumor effect.

In order to investigate the tumor growth inhibition in vitro, the cell viability of the NPs with or without RF treatment were investigated. As shown in Figure 7A, the PLGA/AuNR@MnO2 showed no significant toxicity to the cells, and there was no significant difference among DTX, PLGA/DTX@MnO2, and PLGA/AuNR/DTX@MnO2 groups at various concentrations (P>0.05). Figure 7B shows that, after RF treatment, the cell viability of each group decreased. However, there was no significant difference between the DTX (or PLGA/DTX@MnO2) group with and without RF irradiation at all the concentrations, (P>0.05). Moreover, the cell viabilities of the groups which contained AuNRs showed a significant difference (P<0.05) between these groups with and without RF treatment, demonstrating that the AuNRs could obviously produce heat when they were irradiated with RF. PLGA/AuNR/DTX@MnO2 drug delivery system could be used for the combined, AuNRs-induced, RF-responsive hyperthermia and chemotherapy.

Figure 7 Cell viability of the NPs with or without RF treatment (13.56 MHz, 400 W, 5 min).
Notes: (A) Cell viability of the NPs without RF treatment. (B) Cell viability of the NPs with RF treatment. The values are expressed as mean ± standard deviation (n=3).
Abbreviations: AuNR, gold nanorod; DTX, docetaxel; MnO2, manganese dioxide; NPs, nanoparticles; PLGA, poly(lactic-co-glycolic acid); RF, radiofrequency.

Cellular apoptosis

The Annexin-V/PI kit was used to substantiate the enhanced apoptosis induced by the MnO2-based formulations. The FCM was performed three times, and the results (Figure 8) showed that the apoptosis rates of DTX, PLGA/DTX/MnO2, PLGA/DTX/MnO2 + RF, PLGA/AuNR/DTX/MnO2 and PLGA/AuNR/DTX/MnO2 + RF groups were 23.3%±1.24%, 24.3%±1.23%, 25.1%±1.72%, 27.2%±1.95%, and 43.8%±2.32%, respectively. Each of the DTX groups, namely the PLGA/DTX/MnO2 and the PLGA/DTX/MnO2 + RF groups, showed no significant difference compared with the others (P>0.05), indicating that PLGA/DTX/MnO2 NPs were not sensitive to the RF irradiation treatment. After RF irradiation, the PLGA/AuNR/DTX/MnO2 + RF group showed higher apoptosis rate than the other groups (P<0.05). In addition to the DTX effect, AuNRs-induced hyperthermia played a key role, demonstrating that the PLGA/AuNR/DTX/MnO2 drug delivery system could induce cellular apoptosis effectively.

Figure 8 Cell apoptosis of the experimental groups.
Notes: (A) Control; (B) DTX; (C) PLGA/DTX/MnO2; (D) PLGA/DTX/MnO2 + RF; (E) PLGA/AuNR/DTX/MnO2; (F) PLGA/AuNR/DTX/MnO2 + RF.
Abbreviations: AuNR, gold nanorod; DTX, docetaxel; MnO2, manganese dioxide; PLGA, poly(lactic-co-glycolic acid); RF, radiofrequency.

Anti-tumor effect in vivo

For the investigation of the anti-tumor effect, the relative tumor volume and the body weights of the mice were detected every other day. Figure 9A showed that the PLGA/AuNR/DTX@MnO2 + RF had obvious anti-tumor effect and had significant difference compared with the other groups (P<0.05). Moreover, compared with the relative tumor volume of PLGA/DTX@MnO2, the PLGA/DTX@MnO2 + RF group showed no significant difference, indicating that the AuNRs played the key role for RF-responsive hyperthermia. As in the DTX group, no weight loss was found in the other groups, suggesting that the toxicity of all the drug delivery systems was negligible (Figure 9B).

Figure 9 Inhibition of tumor growth in vivo by the drug delivery system.
Notes: (A) Relative volumes of tumor-bearing mice; (B) body weights of the mice models (mean ± standard deviation; n=6); (C) H&E-stained tumor tissue sections: (a) control, (b) DTX, (c) PLGA/DTX/MnO2, (d) PLGA/DTX/MnO2 + RF, (e) PLGA/AuNR/DTX/MnO2, and (f) PLGA/AuNR/DTX/MnO2 + RF.
Abbreviations: H&E, hematoxylin and eosin; AuNR, gold nanorod; DTX, docetaxel; MnO2, manganese dioxide; PLGA, poly(lactic-co-glycolic acid); RF, radiofrequency.

For further detection of the anti-tumor effect, the cell morphology of the tumor tissues was investigated by using H&E staining method (Figure 9C). The H&E sections of PLGA/AuNR/DTX@MnO2 + RF group showed high cellular polymorphism, cell necrosis, cell lysis, and cell fragment. The cell morphologies of the DTX, PLGA/DTX@MnO2, PLGA/DTX@MnO2 + RF, and PLGA/AuNR/DTX@MnO2 groups were similar, and the level of cell deformation was less than that in the PLGA/AuNR/DTX@MnO2 + RF group. The results indicated that the combination therapy of AuNRs-induced RF hyperthermia and DTX-induced chemotherapy could be used to achieve efficient tumor inhibition.


In order to investigate the biodistribution of DTX alone or PLGA/AuNR/DTX@MnO2 drug delivery system, the HPLC method was used in this study. As shown in Figure 10, it was observed that a significant difference existed between the DTX and PLGA/AuNR/DTX@MnO2 groups. DTX-alone group was mainly distributed in liver, lung, and kidney, where PLGA/AuNR/DTX@MnO2 was mainly distributed in lung and liver organs. Importantly, the uptake of DTX in tumor was significantly higher in the PLGA/AuNR/DTX@MnO2 group than in the DTX group at 4, 6, and 8 h, respectively (P<0.05). In addition, the biodistribution of PLGA/AuNR/DTX@MnO2 was the highest at 4 h after administration.

Figure 10 Biodistribution of the drug delivery system at 1, 2, 4, and 8 h, respectively.
Notes: (A) DTX; (B) PLGA/AuNR/DTX/MnO2. The values are expressed as mean ± standard deviation (n=6).
Abbreviations: AuNR, gold nanorod; DTX, docetaxel; MnO2, manganese dioxide; PLGA, poly(lactic-co-glycolic acid).

Effects of MRI and X-ray imaging in vivo

The diagnostic imaging of the tumor is very important for prognosis, and it is good for the real-time tracking of the therapeutic effect of the drug delivery system. Nowadays, a number of theranostic drug delivery systems have been prepared and investigated in vitro and in vivo.4345 In the previous studies, it was found that Mn2+ and gold nanomaterials can be used for MRI and X-ray CT imaging for the early diagnosis of cancer, respectively.41,46 Gold nanoshells and Fe3O4 NPs have been used to prepare a core–shell structured delivery system that had the dual-mode imaging effect.33 Kim et al47 used Mn2+ and Fe3O4 to prepare a delivery system, showing the T1/T2 dual-mode MRI effect. Nowadays, in cancer research, a number of dual-mode imaging drug delivery systems have been constructed for tumor theranostic applications48,49 as they have more advantages. On the one hand, the dual-mode imaging can be more accurate to determine the real-time location of the drug delivery system. For better use of RF hyperthermia, the time point of treatment is very important. It is possible to determine the maximum distribution of the drug delivery system at the tumor site by using this imaging method. On the other hand this method can also detect the best time point for hyperthermia.

MRI and CT are the most commonly used and efficient imaging methods in clinical treatments. In this study, dual-mode imaging (MRI and X-ray CT imaging) was used for tumor diagnostics (Figure 11). PLGA/AuNR/DTX@MnO2 solution was injected through the mouse tail vein, and the results were obtained at 4 and 8 h after injection. It can be seen that the administration of PLGA/AuNR/DTX@MnO2 enabled a clear MR or CT contrast imaging at the tumor site, and both the MRI and CT results showed clearly that the biodistribution of PLGA/AuNR/DTX@MnO2 in the tumor site was the highest at 4 h after administration (Figure S2). It was shown that this drug delivery system had great potential in tumor dual-mode imaging applications.

Figure 11 Dual-mode imaging in vivo.
Notes: (A, B) T1-weighted MR images; (C) X-ray CT images: (a) control; (b) 4 h; (c) 8 h.
Abbreviations: MR, magnetic resonance; CT, computed tomography.


In this study, the PLGA/AuNR/DTX@MnO2 drug delivery system was constructed and investigated in vitro and in vivo. The AuNRs with different ARs were prepared first, and it was proven that they could be used for RF-induced hyperthermia. All the results indicated that the PLGA/AuNR/DTX@MnO2 drug delivery system could be used for chemotherapy, hyperthermia, controlled release, and dual-mode imaging. The PLGA/AuNR/DTX@MnO2 delivery system has great potential to be used in tumor theranostic applications.


This work was supported by the National Natural Science Foundation of China (Nos 81673021, 81573151, 81572991, and 81302717), the China Postdoctoral Science Foundation (Nos 2014M562002 and 2015T80783), and Outstanding Young Talent Research Fund of Zhengzhou University (No 1421331073).


The authors report no conflicts of interest in this work.



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Supplementary materials

Figure S1 TEM image of PLGA/AuNR/DTX@MnO2.
Abbreviations: TEM, transmission electron microscopy; AuNR, gold nanorod; DTX, docetaxel; MnO2, manganese dioxide; PLGA, poly(lactic-co-glycolic acid).

Table S1 Particle sizes
Abbreviations: AuNR, gold nanorod; DTX, docetaxel; MnO2, manganese dioxide; PBS, phosphate-buffered saline; PLGA, poly(lactic-co-glycolic acid).

Table S2 Zeta potential
Abbreviations: AuNR, gold nanorod; DTX, docetaxel; MnO2, manganese dioxide; PLGA, poly(lactic-co-glycolic acid).

Figure S2 (A) HU value of the tumor at 4 and 8 h after the injection of PLGA/AuNR/DTX@MnO2; (B) the relative signal intensity of the tumor at 4 and 8 h after the injection of PLGA/AuNR/DTX@MnO2.
Note: The values are expressed as mean ± standard deviation (n=3).
Abbreviations: AuNR, gold nanorod; DTX, docetaxel; MnO2, manganese dioxide; PLGA, poly(lactic-co-glycolic acid); HU, Hounsfield units; MRI, magnetic resonance imaging.

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