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Forensic mycology: current perspectives

Authors Hawksworth DL , Wiltshire P 

Received 15 August 2015

Accepted for publication 29 October 2015

Published 10 December 2015 Volume 2015:5 Pages 75—83


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Professor Henrik Druid

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David L Hawksworth,1–4 Patricia EJ Wiltshire4,5

1Department of Plant Biology II, Faculty of Pharmacy, Complutense University of Madrid, Madrid, Spain; 2Comparative Plant and Fungal Biology, Royal Botanic Gardens, Kew, Surrey, 3Department of Life Sciences, The Natural History Museum, London, 4Geography and Environment, University of Southampton, Southampton, 5Department of Geography and Environment, University of Aberdeen, Aberdeen, UK

Abstract: Mycology can contribute to a variety of forensic investigations, including the determination of postmortem intervals from mold growth on corpses, psychoactive substances and toxins, hazards from mold growth in buildings, and providing trace evidence linking people and objects with places. Studies are also starting to be undertaken to explore the use of molecular data on fungi in the characterization of soils. In addition, where there are health concerns, possibly associated with mold growth in buildings, guidance is presented with respect to the collection of samples, and some caveats are given which must be considered in interpretation of data. Attention is drawn to pertinent publications which either appeared, or came to the attention of the authors, since the review they prepared in 2010. This is supplemented by examples from their own recent casework. In order to avoid valuable information being overlooked, there is a need for investigating officers, and those involved in forensic medicine, especially pathologists and toxicologists, to be aware of the evidential value of fungi. In particular, they should not overlook opportunities to recover spores from human remains, to examine any mold colonies growing on corpses, and to analyze gut contents for fungal material and spores.

Keywords: fungi, mold, pollen, postmortem interval, spores, toxicology

Opening comments

It is gradually becoming more widely appreciated that mycology (the study of fungi) contributes greatly to the provision of intelligence, and to the resolution of evidence in various kinds of criminal and civil forensic investigations.

A review, illustrating the range of applications of mycology in actual criminal investigations, was prepared in 2010,1 and the present contribution draws attention to additional examples and experimental studies, especially ones published since that date. Also, we now extend the scope to civil investigations which have a health or medical aspect. This review is written for coroners, pathologists, toxicologists, and investigators concerned with both criminal cases and insurance claims. Through drawing attention to what has been achieved in actual cases, it aims to provide guidance as to where specialist mycological input could be beneficial. Some common pitfalls in sampling and interpretation, particularly in investigations of mold growth in buildings, and ingestion of psychotropic and toxic substances, can lead to unsound claims of adverse medical effects from fungi.


Our first review of forensic mycology1 focussed on its application in actual criminal cases; the review endeavored to cover literature and casework to mid-2010. This follow-up contribution draws attention to work carried out since that time, and a few studies of which we were unaware previously. We also extend the scope to issues concerned with civil investigations where there are medical implications.

The review of forensic mycology by Said Al Na’imi2 came to our attention after our first review appeared; it is complementary in that it also covers aspects of mycology that are not involved with criminal investigation.

Postmortem interval

There is continuing interest in the possibilities of using fungi and prokaryotes (bacteria) associated with human decomposition to indicate postmortem interval (PMI).3 A microbial clock has been developed using culture independent high-throughput sequencing in a model system, in which mouse corpses were placed on soil and decomposition monitored for 48 days. During the observation, samples were taken from the grave soil, abdominal cavity, and skin.4 The data from bacterial groups and a nematode, rather than any fungi, enabled a prediction to be made with an accuracy of approximately 3 days in this model system. The authors suggest that the method may be developed into a forensic tool although, in the case of human burials, there are so many physicochemical interacting variables (such as soil type, chemistry, redox potential, and water availability),5 it is difficult to envisage how any robust estimates of evidential value can be expected from this approach in the foreseeable future.

Direct observations on human cadavers buried in soil in Russia demonstrated that soil fungi were able to grow through the skin and clothes by the second week after burial, that the fungi involved were authochthonous (authochthonous: belonging to that place), and that they corresponded to those recovered directly from the burial places.6,7 The reported fungi were all common soil fungi, and were only named to genus. This Russian research group extended their mycological studies and, by combining them with entomological data, developed criteria for the estimation of PMI of bodies buried in soil, salt, and freshwater.8

Fungal cultures were grown from samples taken from 60 human corpses in different stages of decomposition (ie, bloated, putrefied, and skeletonized), obtained from a morgue, fields, or exhumed in the state of Ceará, Brazil.9 Fungi were cultured from mucosal swabs, skin scrapings, hair, and lung biopsies; and also from clothing, coffins, and nearby soils. The organisms recovered were mainly saprobic molds (eg, Aspergillus, Mucor, Penicillium), keratinophilic fungi (Trichosporon), or yeasts (Candida albicans and C. parapsilosis). During the decomposition of the bodies, the greatest number of isolates was obtained from the bloated stage (143 cultures), compared with the putrefied (12), and skeletonized (26) stages. In the case of the associated clothing, coffins, and soil, 67 isolates were recovered from the skeletal stage, and none from the others. The authors concluded that, while differences in range of fungal isolates were found, they could not be used as effective biological markers of time of death.

In contrast to approaches based on the isolation and culture of fungi from human remains, the sizes of fungal colonies developed on skin (Figure 1), or other materials, have already been used successfully to estimate times of death or deposition.1 Examples of species we have found to be of particular value in this context are given in Table 1. We can now add to the cases to which we previously drew attention.

Figure 1 Greenish yellow patches of the fungus Penicillium griseofulvum developed directly on the skin of a torso of a human cadaver.

Table 1 Examples of mold species found on human skin of value in the estimation of postmortem interval

A young woman was found dead in a flat in West Yorkshire in January 1996. The forensic pathologist noted that there was some mummification and skin slippage, and it was necessary to determine how long the woman had been dead before her body was discovered. There were bluish green and gray mold colonies present, and the West Yorkshire Police Support Service took swabs from these, and then measured the sizes of the bluish green colonies; the gray ones were considered too diffuse to measure. The molds were identified as Penicillium and Mucor, but were not named to species. Over a period of 28 days, an experienced medical mycologist studied the growth rates of the fungi on two artificial media at a temperature similar to those where the body was found (ca 4°C), and at room temperature (ca 21°C–24°C). After comparing the growth rates, the mycologist concluded that “death occurred a minimum of 3–4 weeks prior to 16 January 1996 when the area of growth on the right cheek was measured”.10 This work contributed to a man being convicted of murder and sentenced to life imprisonment.

Fungal growth was reported on the surface of dismembered body parts of a fetus abandoned in a vineyard in Portugal. The growth of fungi led to the conclusion that the body had been stored in a confined space; it emerged that the body had been kept in a plastic bag in a refrigerator for some months prior to deposition.11

In the case of a young woman raped and murdered in Dundee, there were extensive fungal growths on exposed parts of the body, and these were evident in photographs taken at the deposition site. The body had been cremated before a mycologist was consulted, but having taken note of the low temperatures in the vicinity over the past weeks, it was estimated that the body had been there at least 2 weeks. Pollen and fungal spore trace evidence had linked a suspect to the victim and deposition site, and the PMI estimate agreed with the time when the victim and suspect had been caught on a video surveillance camera. The suspect had 13 previous convictions for sexual offences, and was sentenced to life imprisonment for the murder.12

Fungi on human remains may respond differently under different circumstances. A corpse left for 6 days in a wood in Italy under conditions of high humidity (80%) and temperature (35°C) in 2014 had been heavily degraded by dipteran larvae (maggots), and much of it was already skeletonized.13 The fungi recovered in this case included Mucor hiemalis and Penicillium pancosmium, and would not have been expected to establish so early in the cooler climates of more northern parts of Europe. The fly larval activity might also have been expected to have had a key role in enabling the fungi to colonize so quickly, so it is evident that every case has to be considered independently.

The extent of mold growth on the remains of cooked food (Figure 2) can also give indications of time. In a flat in northeast London in 2013,14 evidence of this type contributed to the conviction of a mother for causing the death of a child by neglect. It was necessary to establish how long the mother had left her three children alone in a locked flat. The fungal colonies were measured and identified, and the results compared with published information on growth rates of the same fungi on artificial media at temperatures similar to those in the flat. The dimensions of the colonies of Aspergillus niger and Geotrichum candidum suggested the food had been left for a minimum of 10–14 days, and not just over a weekend as at first claimed; the mother pleaded guilty.

Figure 2 Plate of an unfinished meal left for 10–14 days showing extensive mold growths. The most conspicuous are Aspergillus niger (black) and Geotrichum candidum (white).

There is evidently a case for investigating officers paying more attention to the sizes of fungal colonies on human remains for estimation of PMI, but the potential of fungi is rarely recognized or considered. Forensic pathologists are often unaware that such growths may be of evidential value and, may clean them from the cadaver without consideration. Comparing fungal colony sizes is of particular value if the fungi can be isolated into culture. They need to be grown in appropriate conditions which mimic, as closely as possible, those prevailing at the place where the body was found. It is not essential to identify any fungus – of critical importance is the response of the species obtained from the body to an experimental temperature range. This means that the comparative work can be undertaken in a basic microbiology laboratory by appropriately trained technicians. In order to encourage the use of the method, recommended protocols are currently being prepared for publication.

Psychotropic and toxic substances

The literature on toxic and psychotropic fungi, their effects, and treatment of cases of mushroom poisonings, continues to grow, and cannot be reviewed in depth here. We do, however, draw attention to publications and issues that can be important in a forensic context, particularly those involving suspicious death. Mushroom poisoning is a particular problem in the People’s Republic of China, and a review of incidents in southern China found there had been 183 deaths out of 852 (21.5%) patients who were hospitalized in the period 1994–2012.15 The number of different fungal species reported to be the cause of fatal poisonings continues to rise, with some mushrooms not previously recognized, or identified, being the cause of fatal incidents. Indications of the ingested species may be gleaned from symptoms13 but toxic fungi may be hitherto unknown species, or ones previously not considered to be poisonous. An example is Pleurocybella porrigens which was involved in 59 cases in 2004, 19 of which were fatal.16 In the case of some fungal toxins, the symptoms they cause may mimic other conditions, such as cerebrovascular accident (stroke).17

With regard to poisonous fungi, the importance of precise identifications by specialists is clear, and this has been highlighted in several studies.18,19 There can be surprises even with supposedly well-known fungi purchased in stores. Recently, molecular work showed that a packet of dried “porcini mushrooms (Boletus edulis)” bought in a London shop, contained a mixture of three Boletus species that were new to science;20 fortunately in this case they appear not to have been poisonous species of the genus.

In toxicological analyses of human tissue samples, checks for poisoning by fungi are usually confined to the determination of the presence or absence of particular compounds implicated in a case. If the specific compounds are not found, investigation proceeds to fungi which are commonly found in the locality in question. Cost is often an issue, and toxicologists may cease further analysis when a significant level of a drug or other substance is detected.21

In a recently reported case,22 dimethyltryptamine (DMT) was found in a sample taken from a man who died 4 days after attending a shamanistic ceremony where he was given Ayahuasca (an infusion made from South American plants from the rain forest). This infusion usually contains Psychotria viridis and often Banisteriopsis caapi. No other toxicological tests on the cadaver were requested by the police, but they had been informed that the deceased was in the habit of using magic mushrooms. A mushroom was found in the dead man’s possession, and it was identified as Psilocybe semilanceata. As in many psychotropic fungi, P. semilanceata produces psilocybin which, in the body, becomes dephosphorylated to the pharmacologically active agent psilocin. To confirm that the deceased had consumed the fungus, contents from his stomach, ileum, and colon were examined microscopically. The colon proved to have abundant spores of the fungus (Figure 3) as well as the pollen of Cannabis, and the seeds of Papaver somniferum (opium poppy). Microscopical analysis of one flask found at the dead man’s home also yielded Cannabis pollen, and another was encrusted with Psilocybe spores. It was established that the man had drunk suspensions of Cannabis and Psilocybe before the Ayahuasca and may have even been trying to obtain opiates from poppy capsules. He had not eaten for 4 days before his death so the seeds were unlikely to have come from bread or other food.

Figure 3 Spores of Psilocybe semilanceata as seen by light microscopy (Nomarski, differential interference contrast); the spores measure 10.5–15 μm × 6.5–8.5 μm.

The police had intended to charge the shaman with manslaughter but, when the mixture of psychotrophic materials was found in his belongings and then in his gut, this was reduced to possession of a Class A drug. This case illustrates the value of analysis by light-microscopy in cases of suspicious death involving mushrooms. It also highlights the wisdom of sampling the lower gut (ileum, colon, possibly rectum, and even feces), as well as stomach contents in cases of suspicious death. Sampling the lower gut is particularly important as symptoms of mushroom poisoning may not become evident for several days after ingestion by which time gut contents may have moved down to the colon and rectum.

Mold growth in buildings

It is generally accepted that extensive mold growth in buildings is correlated with respiratory health issues,23,24 but establishing cause and effect in any particular case is challenging.

Where there has been illness or death of individuals living in moldy buildings, the fungi deemed to have been the cause should be investigated before the case is dismissed. The sensitivity of any affected individual to specific fungal allergens, or the possibility of fungal growth in the lung, should always be checked. The forensic mycologist may be called upon to assist in such investigations, and sampling should be appropriate.

Considerable emphasis has been placed on sampling of aerial fungal spores in attempts to establish risks and correlations, but this method is problematical. It has been demonstrated that the airspora (airspora: pollen, plant spores, fungal spores [and other microscopic entities] in the air at any one time) fluctuates considerably both diurnally and seasonally.25 Further, some fungi are slime-spored rather than dry-spored, and do not readily discharge spores into the air. Others may be of concern because of the production of volatile compounds.

Great care must be taken to ensure that the species, developed on culture media from samples obtained from the building, are those growing in situ. Media are selective, and adventive spores may be cultured from the samples, rather than those produced by fungi actually growing in the property. Any growing colonies should be sampled by in situ taping; this will confirm any observations made by other sampling methods.26 Examples of some molds associated with indoor damp problems are shown in Table 2. Temporal estimates for colony growth can be made by using the same techniques as those described for mold growth on human remains and/or associated objects (see Postmorten interval section). The dimensions of colony growth in vitro, under known temperatures, and for known time periods, can then be compared with those in the property; and a minimum age for colonization (Figure 4) can be estimated. This has provided particularly important information for evaluating whether insurance claims for water damage are fraudulent or valid.

Table 2 Examples of mold fungi associated with indoor damp problems

Figure 4 Colonies of Stachybotrys chartarum, the so-called “toxic mold”, developed on a vertical plastered wall affected by rising damp.

Soil profiles

In the light of recent advances in molecular methods, it might be expected that they would be useful for the characterization of soil samples recovered from cadavers and objects, and linking them with specific locations.1 This is not an issue solely for forensic investigations, but for soil molecular ecology as a whole. An increasing number of techniques are becoming available,27 but fundamental issues need to be resolved before any results could be considered sufficiently robust for use in the forensic context.28

There has been some attempt to standardize the extraction of microbial DNA, even to the extent of the production of an ISO (International Standards Organization) standard, but a protocol for use in forensic laboratories is not yet available:29 “The problem at present is that it is difficult to reproduce the same result from soil samples”,30 and “analyses of replicates is undertaken in less than one third of molecular ecology papers”.31 Some success with bacteria has been claimed in a forensic case by comparing restriction digest fingerprints rather than sequences32 but, with fungi, there are major concerns regarding the use of high-throughput sequencing to assess fungal communities.33 Methods are becoming increasingly sophisticated as metagenomic approaches develop, and those interested in exploring molecular methods for assessing fungi in soil samples, are encouraged to follow the development of the molecular ecology literature.34

Trace evidence

An early, unpublished, use of lichen material as trace evidence has recently become known. Fragments of the lichen Lecanora dispersa, were found on trousers of one of three men involved in a factory break-in Coventry in 1962. The lichen fragments formed part of the evidence that helped the West Midland Forensic Science Laboratory establish they had entered the property over a concrete wall.35

Several cases in which fungal spores and remains had been used as trace evidence in criminal investigations were reported on previously,1 and a fuller account of one of those, a rape in Wiltshire, has now been published.36 In many cases, DNA may not be available or, as in the case of consensual vs rape, it may be irrelevant. The rape in Wiltshire demonstrates that, when combined with palynological results, fungal data are especially valuable.

We continue to use fungal data in investigations to assist police forces, and mention two additional illustrative examples here. In the case of a rape and murder in Dundee,12 comparison of the independent pollen and fungal spore profiles retrieved from 1) the victim’s body, 2) the place of deposition, and 3) shoes of the suspect, were crucial in securing a conviction for murder. The scene was unusual in being planted with exotic tree and shrub species, and the local municipal authorities had covered the ground with macerated woody mulch derived from garden waste, parks, and gardens; the mulch consisted of a unique mixture of woody material. Sixteen fungi were common to the shoes of the suspect, the clothing of the victim, and the crime scene. It was of particular note that the fungal remains included minute shield-like sporophores (spore producing structures) from three species, and these could only be expected to be picked up by a direct physical contact.

In the second case, an elderly man and wife were badly injured by assailants during an aggravated burglary in Lincolnshire. The couple made their living by selling firewood bundles made up from trees cleared from other properties, especially gardens and estates. Data obtained from the various palynological profiles suggested that much of the trace particulate material was in common with the crime scene rather than the alibi sites described by the suspects. They claimed that all the relevant pollen and spores had been picked up from a local farm, and an adjacent fishing lake. There were 65 different taxa of fungal spores recognized in the case material (Table 3) and many were retrieved from the vehicle and boots of the two suspects. Of 29 different kinds of fungal spores found at the crime scene, 28 were obtained from the boots and escape vehicle,37 and none of these was found in the alibi samples. One of the critical species, Testudina terrestris, was a fungus only occurring on conifers alien to the UK and, until it was found in this case, had only previously been reported from the Royal Botanic Gardens Kew. The fungal and palynological data corroborated each other; the suspects pleaded guilty and received custodial sentences.

Table 3 A summary of fungal palynomorphs from the crime scene, and alibi site, a car used in the escape, and the boots of each of the two suspects, in an aggravated burglary
Notes: Figures represent numbers of fungal spores expressed as a percentage of total non-fungal palynomorphs, plus fungal spores. Plus signs indicate values <0.3% of the total count. “Type” indicates an informal morphological spore category in which the spores have not been identified; the letter codes used are arbitrary identifiers. Data from the report for Lincolnshire Police.37

The identification of fungal spores can be a time-consuming and so, a costly process. It may involve extensive literature searches, comparison with preserved reference collections, and sending pictures to specialists on specific fungal groups around the world. However, whether the spores are named or not, if identical ones are obtained from different exhibits, they can still be of value in a case. Identification is preferred because if they are named, they can provide additional value with respect to the substrates and/or host plants on which they are known to grow. This may provide ecological information on both the plants and soils, and might facilitate the elimination of certain locations in an investigation. Precise identification makes it possible to check how commonly any species is reported in pertinent databases. In the UK, the Fungal Records Database of Britain and Ireland ( is especially valuable; it contained 1,984,344 records on 9 August 2015.

We mention the use of fungal spore data as trace evidence here as, in addition to the issue of forensic pathologists cleaning away visible mold colonies from human remains (see Postmorten interval section), they need to be aware that there may be fungal spores, pollen, and other microscopic particulates, of potential evidential importance in hair, exposed tissues, nasal passages, mud smears, and attached vegetation. Protocols for the recovery and processing of palynomorphs (ie, pollen, spores, and other microscopic entities), and use as trace evidence from environmental samples, human remains, and various objects, are available separately.38


From this review of the use of fungal data in a broad spectrum of forensic situations, it will be evident that mycology has much to contribute to the investigation of both criminal and civil cases (in the latter, particularly for insurance fraud). The applications discussed here are not suggestions of possibilities yet to be explored, but ones which have been used in actual cases and tested in the Courts. In order further to realize the potential, however, it will be necessary for investigating officers to become more aware of the possibilities provided by spores and whole fungi, and to recognize situations where mycological evidence might provide critical evidence. In such cases, specialist advice should be sought. There is a need for those involved in all aspects of forensic medicine (especially pathology and toxicology), to be aware of the evidential value of fungi, especially with respect to trace evidence, timing of events, and demonstrating psychotropic substances, and poisons. They may provide important evidence which might otherwise be missed.


The authors report no conflicts of interest in this work.



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