Flow chamber analysis of size effects in the adhesion of spherical particles

P Decuzzi^1,3, F Gentile¹, A Granaldi³, A Curcio², F Causa¹, C Indolfi², P Netti⁴, M Ferrari⁵

¹Center of Bio-/Nanotechnology and -/Engineering for Medicine and ²Division of Cardiology, University of Magna Graecia at Catanzaro, Viale Europa – Loc. Germaneto, Catanzaro, Italy; ³Center of Excellence in Computational Mechanics, Politecnico di Bari, Bari, Italy; ⁴Interdisciplinary Research Centre in Biomaterials, University of Naples – Federico II, Naples,  Italy; ⁵The University of Texas Health Science Center, and M.D. Anderson Cancer Center and Rice University, Houston, Texas, USA

Abstract: The non-specific adhesion of spherical micro- and nano-particles to a cell substrate is investigated in a parallel plate flow chamber. Differently from prior in-vitro analyses, the total volume of the particles injected into the flow chamber is kept fixed whilst the particle diameter is changed in the range 0.5–10 µm. It is shown that: (i) the absolute number of particles adherent to the cell layer per unit surface decreases with the size of the particle as d^−1.7; (ii) the volume of the particles adherent per unit surface increases with the size of the particles as d^+1.3. From these results and considering solely non-specific particles, the following hypothesis are generated (i) use the smallest possible particles in biomedical imaging and (ii) use the largest possible particles in drug delivery.

Keywords: bio-adhesion, particulates, flow chamber, in-vitro experiments