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Five Serum Trace Elements Associated with Risk of Cardia and Noncardia Gastric Cancer in a Matched Case–Control Study

Authors Lin Y , Wu C , Yan W, Guo S, Liu B

Received 20 February 2020

Accepted for publication 23 March 2020

Published 10 June 2020 Volume 2020:12 Pages 4441—4451

DOI https://doi.org/10.2147/CMAR.S250592

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Professor Rudolph Navari



Yulan Lin,1 Chuancheng Wu,2,3 Wei Yan,4 Saixiong Guo,4 Baoying Liu2,3

1Department of Epidemiology and Health Statistics, School of Public Health, Fujian Medical University, Fuzhou 350122, Fujian, People’s Republic of China; 2Department of Preventive Medicine, School of Public Health, Fujian Medical University, Fuzhou, Fujian, People’s Republic of China; 3Fujian Provincial Key Laboratory of Environment Factors and Cancer, Fujian Medical University, Fuzhou, People’s Republic of China; 4Fujian Xianyou County Hospital, Putian, People’s Republic of China

Correspondence: Chuancheng Wu; Yulan Lin Email [email protected]; [email protected]

Background: Serum trace elements have for some time been suggested to influence the development of gastric cancer, but evidence is still lacking.
Methods: All newly diagnosed patients with gastric cancer were compared with healthy controls 1:1 matched by sex, age (± 3 years), and place of residence during 2013– 2015. The serum concentration of all trace elements was analyzed using inductively coupled plasma mass spectrometry. Heliobacter pylori infections in cases were diagnosed using the rapid urease test, while in controls were detected using the colloidal gold method.
Results: A total of 122 cases of gastric cardia cancer (GCC) and 177 gastric noncardia cancer (NCGC), and 299 matched healthy controls were included. Positive associations were found between serum copper and copper/zinc ratio and risk of overall gastric cancer (OR4th vs 1st quartile: 2.42, 95% CI: 1.10– 5.32 for copper; OR4th vs 1st quartile: 11.7, 95% CI: 3.83– 35.6 for copper/zinc ratio), and for both GCC and NCGC subtypes. Serum selenium was inversely associated with the risk of NCGC (OR4th vs 1st quartile: 0.18, 95% CI: 0.07– 0.51), while molybdenum seems to reduce the risk of GCC (OR4th vs 1st quartile: 0.12, 95% CI: 0.03– 0.47). Strong inverse associations were also detected between serum calcium and risk of all groups of gastric cancer (all p for trend< 0.05).
Conclusion: The current study indicated statistically significant positive associations between serum copper, copper/zinc ratio, and gastric cancer, as well as inverse associations among selenium, molybdenum, and calcium. The results shall be carefully interpreted before further in vivo animal studies show definitive evidence.

Keywords: gastric cancer, serum trace elements, nutrition, environment

Introduction

Although the incidence has been decreasing in the past decades, gastric cancer remains globally the third most frequent cause of death from cancer. Incidence varies greatly among populations and is higher in Asian and Central and South American countries but lower in North America and Western Europe.1 Migration studies found lower incidence of gastric cancer among immigrants moved from high-risk regions to low-risk regions, which indicated that environmental exposures, such as food, could play an important role in the development of this disease.2,3 Furthermore, higher incidences of gastric cancer were usually found in those regions with more infertile lands, high mountains, or excessive heavy metals levels in soil, cereals, and drinking water. A previous study confirmed that this environmental pollution by trace elements might contribute partly to their high incidence of gastric cancer.4

Some early studies have investigated the role of trace elements in the development of gastric cancer, but the results were inconsistent and the mechanisms of the effect of trace metals on gastric cancer are still far from being elucidated completely. Copper (Cu), zinc (Zn), selenium (Se), iron (Fe), molybdenum (Mo), magnesium (Mg), and calcium (Ca) are essential trace metals and components of critical enzyme systems, playing key roles in maintaining DNA integrity by preventing oxidative DNA damage or affecting gene mutation.57 The main sources of these trace elements are food and drinking water. The variations in these mineral levels were related to the incidence of several cancers. For example, Se has been studied for antioxidant and anticancer properties,8,9 especially against gastric cancer.10 Inverse associations were also obtained between serum Zn and risk of gastric cancer in some early studies.11,12 A higher level of Cu alone was also found significantly higher in cancerous human stomach compared to normal samples.13 More importantly, Zn and Cu are known as key cofactors in several enzymes, which include Cu/Zn superoxide dismutases (SODs) decomposing superoxide radicals to H2O2 and playing a significant role against oxidant stress in several DNA repair proteins.14,15 Surprisingly, a previous study has demonstrated that high serum levels of Cu/Zn superoxide dismutase can be related to an increased gastric cancer risk.16 The association between Cu/Zn ratio and risk of gastric cancer is largely unknown and therefore needs further investigation. Although it seems biologically plausible for Fe to be associated with gastric carcinogenesis, the evidence is rather heterogeneous and insufficient to lead to any conclusions.17

Xianyou County in Putian City is located between east longitude 118°27ʹ–118°56ʹ and north latitude 25°11ʹ–25°43ʹ on the southeast coast of Fujian province, China, and had an estimated population of 2.1 million in 2012. In this county, gastric cancer was the dominant leading cause of death, explaining 30.43% of total cancer death.18 The standardized mortality rate of gastric cancer was 49.47/100,000 in Xianyou County, which was 2.23-fold higher than the Chinese national average (21.9/100,000).18 Mountain areas were observed to have the highest mortality rate (94.4/100,000), in comparison to the lowest rate observed in coastal regions (26.4/100,000) (data not shown). In line with this finding, an earlier study also confirmed a lower serum level of Zn and a higher level of Cu/Zn ratio in the healthy population in Putian City compared to that of other regions in Fujian province with lower incidence of gastric cancer.19 This evidence indicates that trace element variations might play a critical role in the development of gastric cancer in this area. However, to date, no large-scale epidemiological studies have proved the association. Thus, the aim of this study was to investigate whether serum concentrations of trace elements are associated with gastric cancer risk, thus to explain partly the high incidence of gastric cancer in Xianyou County.

Materials and Methods

Study Design and Population

A population-based case–control study was conducted in Xianyou County between March 2013 and December 2015. All newly diagnosed cases of gastric cancer in Xianyou County Hospital were identified. The case identification was performed by clinical research nurses recruited and trained specifically for the study. All individuals who were aged ≥18 years and have lived in Xianyou for at least 10 years were eligible and recruited in Xianyou County Hospital. Gastric cancer was defined according to the International Classification of Diseases, 10th Revision (ICD-10) code, and was subclassified as gastric cardia cancer (GCC) if it involved the esophagogastric junction and as noncardia gastric cancer (NCGC) if it did not. Patients who have started cancer treatment, had a history of any cancer, have mental illness, and are diagnosed with diabetes and those unable to complete the questionnaire were excluded. Controls were individually matched (1:1) to cases based on sex, age (±3 years), and place of residence. They were required to live in the same town or a town with a similar incidence of gastric cancer, and were required to be living in Xianyou County for at least 10 years with no prior history of gastric cancer. The study was approved by the ethics committees at Fujian Medical University. Written consents were obtained from all participants at study enrollment.

Questionnaire Data Collection

All participants were personally face-to-face interviewed by trained research nurses from Xianyou County Hospital. A structured and standard questionnaire consisting of 337 items under 8 categories was used for the data collection. The questionnaires included the following categories: (i) demographic factors (16 items), including the subject’s age, height, weight, marital status, education level, monthly income, etc.; (ii) occupational history (17 items), including profession, pesticide exposure at work, etc.; (iii) lifestyles (70 items), including smoking habits, use of alcohol, tea consumption, etc.; (iv) dietary intake (140 items); (v) personal medical history (33 items), including personal disease records, medication, etc.; (vi) family cancer history (18 items), including family history (first and second-degree relatives) of any cancer, gastric cancer, and esophageal cancer; (vii) mental health (9 items); and (viii) cancer-related diagnoses and treatment (34 items), including surgery, chemotherapy, and radiotherapy, etc. Smoking habit was defined as consuming at least one cigarette per day during the last 6 months or longer.

Blood Sampling

Fasting blood samples were collected from all study participants in the morning in Xianyou County Hospital. Approximately 5 mL of venous blood was collected with ethylenediaminetetraacetic acid (EDTA) as an anticoagulant for erythrocyte preparation and serum. EDTA tubes were centrifuged for 10 min at 3000 rpm, and the serum sample was separated and kept in trace element-free tubes and stored at −80 °C prior to analyses.

Detection of the Concentrations of 7 Trace Elements

The serum concentration of all trace elements was analyzed using inductively coupled plasma mass spectrometry (ICP-MS; Perkin Elmer NexION 350X, USA). The ICP-MS minimal detection cut-off was 0.5 ng/L. Deionized water (18.2 MΩ.cm), which was gained from a Milli-Q water purification system (Millipore Co., USA), was used throughout the experiment. Up-s grade (ultrapure) nitric acid (HNO3) was purchased from Suzhou Crystal Clear Chemical Corporation (Suzhou, China). Internal standard solution (100 μg/mL) (PE#: N9400381) was purchased from Perkin Elmer Life and Analytical Sciences (Shelton, CT, USA).

The preparation of serum samples before ICP-MS analysis followed the standard procedure of microwave digestion methodology (Supplemental Table 1). For quality control, we conducted an additional recovery test to monitor measurement accuracy, and also parallel tests to check the precision among randomly selected samples. For the recovery test, each serum sample was divided in half, one of which was analyzed with the mixed serum sample. The recovery rate ranged between 95.18% and 109.24% (Supplemental Table 2). To test the precision of measurement, each selected serum sample was divided into 5 small samples, and 5 parallel tests were performed under the same experimental conditions. The relative standard deviations measured among 7 elements ranged from 0.56% to 9.71%.

Measurement of Heliobacter pylori Infection

All GC cases underwent gastroscopy in Xianyou County Hospital, and H. pylori infections for these cases were diagnosed using the rapid urease test (RUT).20 Meanwhile, H. pylori infection for controls was detected using the colloidal gold method through identifying urease in a blood sample.21 The colloidal gold method is a simple and noninvasive approach, which has been widely evaluated with high accuracy compared to traditional tools.21 In a recent Chinese multicenter study evaluating H. pylori infections for 378 participants, the sensitivity, specificity, positive predictive value, negative predictive value, and concordance rate of the colloidal gold method were 84.4%, 92.6%, 96.0%, 73.7%, and 87.0%, respectively, in comparison to the “gold standard” 13C urea breath test (UBT).22

The procedure of the colloidal gold method has been described in an earlier study.22 In brief, 2–3 drops of blood were mixed with sample diluent, and the results were evaluated within 5–8 minutes by reading red color bands. The presence of 2 red color bands (“T” band and “C” band, ie, testing band and control band) within the result window indicated a positive result. The presence of only 1 color band indicated a negative result. All assessment kits were purchased from Shanghai Kaichuang Biotechnology Co., Ltd from Shanghai, China.

Statistical Analysis

Since most of the serum concentrations of trace elements were not normally distributed, we used the median and interquartile range to describe the distributions. The T-test (normally distributed) and Wilcoxon rank-sum test (non-normally distributed) were used for the comparisons of continuous variables. To assess the effect of trace elements on the risk of gastric cancer, conditional logistic regression was used to estimate adjusted odds ratios (ORs) and their 95% confidence intervals (CIs). Serum concentrations of trace elements were classified into quartiles based on levels of controls. We adjusted for potential confounding factors including age group (<55, 55–64, 65–74, ≥75 years), sex (male, female), body mass index (BMI; <18,5, 18.5–24.9, ≥25 kg/m2), education level (≤7, 7–9, ≥10 years), occupation (farmer, industrial worker, office worker, others), household income (<600, 600–1000, ≥1000 Chinese Yuan/month/person), smoking (yes, no), chronic atrophic gastritis (yes, no), family history of gastric cancer (yes, no), and H. pylori infection (yes, no) when applicable. We also conducted subgroup analysis according to tumor localization: GCC vs NCGC using conditional logistic regression, and applied the Wald test to assess heterogeneity of the ORs. Two-sided p<0.05 was regarded as having statistical significance. SAS 9.4 was used for all statistical analyses.

Results

Baseline

A total of 299 cases of gastric cancer were included in the final analysis, consisting of GCC (n=122) and NCGC (n=177) patients (Table 1). There were 96 patients with gastric cancer excluded due to the baseline diagnosis of diabetes. Both GCC and NCGC cases had lower levels of BMI, education, and monthly household income compared to controls (all p<0.05). Higher prevalence of chronic atrophic gastritis was observed in both GCC (29/122, 23.8%) and NCGC (13/177, 18.6%) compared to controls (22/299, 7.4%) (all p<0.05). The prevalence of H. pyloriinfection was observed lower in patients with GCC (31.6%), compared to control participants (45.1%).

Table 1 Baseline Characteristics of Gastric Cancer and Control Participants in a Chinese Matched Case–Control Study

Distributions of Trace Elements

Median serum concentrations of Cu were observed much higher in both GCC (1014.6 μg/L) and NCGC (1000.2 μg/L) cases compared to controls (927.7 μg/L) (all p<0.05) (Table 2). The distributions of Zn and Se were similar between controls and all gastric cancer cases (all p>0.05). On the other hand, median serum concentrations of Mo, Fe, and Ca were all higher in gastric cancer groups than in controls.

Table 2 Distribution of Serum Level (Median and Interquartile Range) of Trace Elements in the Study Population (n=594)

Serum Trace Elements in Relation to Gastric Cancer

The highest level of Cu was observed with an OR of 2.42 (95% CI: 1.10–5.32) for overall gastric cancer risk compared to the lowest reference group (Table 3). Dose–response correlations were observed between the serum level of Cu and all subtypes of gastric cancer (all p for trend<0.05). Study subjects with an intermediate level of Zn (quartile 3: 650.2–844.5 μg/L) were observed with lower risk of gastric cancer combined (OR4th quartile=0.25, 95% CI: 0.10–0.61), GCC (OR4th quartile=0.15, 95% CI: 0.03–0.43), and NCGC (OR4th quartile=0.34, 95% CI: 0.13–0.98). However, no dose–response association was observed in any of the case groups (all p >0.05).

Table 3 Risk Assessment of Gastric Cancer, Expressed as Odds Ratio (OR) and 95% Confidence Interval (CI), in a Chinese Case–Control Study

The highest serum level of Se was inversely associated with risk of gastric cancer combined (OR4th quartile=0.33, 95% CI: 0.15–0.75), and this association seems limited to the NCGC subtype (OR4th quartile=0.18, 95% CI: 0.07–0.51). No associations were, however, observed between risk of GCC and Se concentration (p for trend=0.43). Comparing the highest to the lowest quartiles of Mo biomarkers, we found statistically inverse associations between this micro-element and overall risk of gastric cancer (OR4th quartile=0.06, 95% CI: 0.02–0.20), as well as GCC (OR4th quartile=0.12, 95% CI: 0.03–0.47). Meanwhile, dose–response associations were found for all case groups (all p for trend <0.05).

No dose–response association was found for Fe in relation to any gastric cancer groups. However, a higher serum level of Fe was observed with more than 50% risk reduction for overall gastric cancer compared to the group with the lowest level of Fe (OR4th quartile=0.41, 95% CI: 0.18–0.91). More specifically, this risk reduction by Fe might only affect GCC patients (OR3rd quartile=0.18, 95% CI: 0.05–0.70). Inverse dose–response associations were found between higher serum concentration of Ca and risk of all gastric cancer subgroups (all p for trend <0.05). Compared to the lowest quartile of Ca, the OR4th quartile calculated in the highest quartile was 0.04 (95% CI: 0.01–0.11) for risk of gastric cancer combined, 0.06 (95% CI: 0.01–0.26) for GCC cases, and 0.02 (95% CI: 0.00–0.17) for NCGC cases.

No significant association was observed for serum level of Mg in relation to gastric cancer risk. But, meanwhile, we observed a strong positive dose–response association between Cu/Zn ratio and risk of all gastric cancer subgroups in the current study (all p for trend <0.05). Study subjects with the highest Cu/Zn ratio had a more than 10-fold increased risk of gastric cancer combined (OR4th quartile=11.70, 95% CI: 3.83–35.6), GCC (OR4th quartile=9.03, 95% CI: 2.29–35.6), and NCGC (OR4th quartile=18.82, 95% CI: 3.50–96.01).

Discussion

The current study mainly reveals the role of serum trace elements in the development of gastric cancer. The results demonstrated strong inverse associations between serum Se, Mo, Ca, and risk of gastric cancer. Meanwhile, high serum concentrations of Cu and Cu/Zn ratio related to increased risk of gastric cancer.

The data from the present study are consistent with the impression that high serum Se may be associated with a decreased risk of gastric cancer. Previous studies have shown that Se may have anti-carcinogenic effects, especially against cancers of the lung, prostate, skin, and gastrointestinal system.23,24 One study observed significantly lower serum levels of Se in the esophageal cancer group, as compared with normal healthy controls.25 A nutrition intervention trial conducted in Linxian County among the general population observed lower mortality of gastric cancer in participant groups receiving supplementation with vitamins and minerals (including Se) than in individuals who did not receive the supplements.26 Of note, Linxian County is also a rural area with poor socioeconomic status and well known for its high incidence of gastric cancer, which is very similar to the study region of the current study.27 Furthermore, it is worth mentioning that the inverse association between Se level and gastric cancer may only occur in populations with low Se levels.28 A serum level of Se below 70 μg/L is usually considered a deficiency.29 According to this standard, 25.4% of our controls (76/299) and 34.1% of all gastric cancer cases (102/299) were considered to be Se deficient. It could be inferred that a low level of Se might contribute partly to the high incidence of gastric cancer found in Xianyou County.

Essential elements Cu and Zn have a role in many biochemical reactions as a micro-source.30 Zn alone has been associated with risk of gastric cancer, but the epidemiological findings have been quite limited and controversial. Zhang et al have observed higher serum concentration of Zn in gastric cancer cases than healthy controls.12 However, a Japanese study found no excess risk of gastric cancer among those with the lowest levels of Zn (OR=1.2).31 Although in our study decreased risks of gastric cancer were found in the third quartile of serum Zn, the linear association was not statistically significant. On the other hand, a higher level of Cu was also observed associated with higher risk of gastric cancer in the current study. Our result is partly supported by previous studies which detected a higher level of Cu in gastric cancer cases compared to healthy controls.32 It has been known that Cu participates in the reductive activation of H2O2, causing damage to cellular DNA, proteins, and lipids. The interaction of H2O2 with Cu generates more reactive oxygen species, such as hydroxyl radicals, eg, • OH. These reactive oxygen species have been considered as being responsible for the process of carcinogenesis.33

There are also controversial data regarding the association between Cu/Zn and several cancers. Our results suggest that the Cu/Zn was positively associated with increased risk of gastric cancer, which was inconsistent with a previous study.16 Similarly, higher levels of Cu/Zn ratio have been previously declared in many types of cancers including head and neck cancer, endometrial cancer, colon cancer, and bladder carcinoma.32,34-37 However, the exact role of these metals in the carcinogenic process has not yet been clearly understood. Cu/Zn ratio and systemic oxidant load have clinical importance for aging-related degenerative diseases, nutritional status, oxidative stress, inflammation, and immune abnormalities which may affect carcinogenesis.14,15,38-40 Mezzetti et al38 reported advanced age, and, particularly, advanced age-related chronic degenerative diseases are associated with a significant increase in the Cu/Zn ratio and systemic oxidative stress. Gaider et al39 stated that higher Cu/Zn ratios correlate with impairments in bone density, physical performance, and overall health in a population of elderly men with frailty characteristics. In peritoneal dialysis patients, elevated Cu/Zn ratios are found to be associated with malnutrition, increased oxidative stress, inflammation, and disrupted immune status.40

Our results found limited evidence for the association between serum Fe status and GCC and all gastric cancer combined, which is partly consistent with those from previous studies. In the European Prospective Investigation into Cancer and Nutrition (EPIC) study, inverse dose–response associations were found between serum Fe and overall risk of gastric cancer (OR4th vs 1st quartile=0.62, 95% CI: 0.44–0.88, p for trend=0.034), as well as NCGC (OR4th vs 1st quartile=0.54, 95% CI: 0.33–0.89, p for trend=0.029).41 Similarly, a Finnish men cohort study also found serum Fe had an inverse or possibly “U”-shaped relationship with this malignancy in the NCGC site (OR3rd vs 1st quartile=0.39, 95% CI: 0.19–0.78, p for trend=0.06), although the trend analysis was not statistically significant.41 In another Finnish cohort study, which involved 120 incident male gastric cancers and 76 female gastric cancers, the relationship in males was statistically significant (RR4th vs 1st quartile=0.60, p for trend<0.01).42

The present study also observed a lower risk of gastric cancer, regardless of GCC or NCGC, in the higher quartile of serum Mo, indicating the protective role of Mo in the development of these diseases. However, little evidence exists to explain the effects of serum Mo on gastric cancer. An earlier Chinese study showed that serum Mo levels of 33 patients with gastric cancer were significantly lower than those of 77 healthy controls.43 Their results also showed a negative correlation (r=−0.285, P<0.01) between soil Mo and the mortality rate of gastric cancer. These results were actually supported by another study, showing that the Mo level in the mucosa of patients with gastritis, gastric ulcer, or gastric cancer was lower than that of normal people.44 These findings indicated that Mo deficiency may be an important risk factor in the development of gastric cancer. Two potential mechanisms have been raised. Mo has been observed with a direct anti-carcinogenic effect on mouse N-nitroso-sarcosine-ethyl-induced foregastric squamosum carcinoma.45 It was assumed that Mo might speed up detoxification of carcinogens through the effect on cytochrome P-450 in cells and demethylase.46 Another potential anti-carcinogenic mechanism could be attributed to the essential role of Mo, a component of nitrite reductase, in the transformation process of nitrate to nitrogen in the environment. Deficiency of Mo in the soil could reduce the conversion of nitrate, and consequently leave more carcinogenic nitrite in the environment. The serum level of Mo could be considered a proxy indicator for the Mo level in the environment. Due to scarce epidemiological data, the appropriate amount of serum Mo in humans warrants further investigations.

Some methodological issues of our study deserve attention. The strengths include the large sample size; individual matching for age, sex, resident region by gastric cancer risk and H. pylori infection status; the standardized tumor classification; the personal interviews with all study participants in a standard and strict manner; and stratified analysis by different gastric cancer localization. Meanwhile, our study has some limitations that should be described. A major concern is the possibility of exposure misclassification using a questionnaire that has not been validated. However, the reliability rate of our questionnaire was estimated as high as 97%, suggesting the potential of high accuracy in measuring risk factors (data not shown). Secondly, the serum level of trace elements based on one single blood sample might also cause some information bias. Further study could be guaranteed by collecting several blood samples at different time points within a day, or different seasons within a year, and using the average level as the true level of a serum trace element. Finally, we were not able to adjust for types of medication taken in the past 1 month. Use of several types of medicines has been observed to influence the serum trace element. For example, an earlier study demonstrated that statin treatment was associated with a significant reduction in mean serum Zn (9%, p=0.03) and Cu (9%, p<0.01).47 Although we did ask participants about the type of medication taken in the past months, only 8 cardia patients, 3 noncardia patients, and 73 controls provided details. Due to the limited responses, we were not able to adjust medication use in the analysis. Further study is warranted with more details on medication taken prior to the data collection.

In conclusion, the study suggested that lower levels of Se, Mo, and Ca and higher levels of Cu and Cu/Zn ratio may play an important role in gastric cancer induction. However, the results shall be carefully interpreted before further in vivo animal studies show definitive evidence.

Abbreviations

GCC, gastric cardia cancer; NCGC, noncardia gastric cancer.

Ethics and Consent Statement

This study was conducted according to the guidelines laid down in the Declaration of Helsinki and all procedures involving human subjects/patients were approved by the Fujian Medical University (ID: 201297). Written informed consent was obtained from all subjects/patients at the study enrollment.

Data Sharing Statement

The datasets analyzed during the current study are not publicly available due to that they concern personal information of study participants, but are available from the corresponding author on reasonable request.

Author Contributions

All authors contributed to data analysis, drafting or revising the article, gave final approval of the version to be published, and agree to be accountable for all aspects of the work.

Funding

This work was supported by the Natural Science Foundation of Fujian Province, China (Grant No. 2015J01673; 2017J01811; 2018J01825); Fujian Innovative Medicine (Grant No. 2016-CX-41); Xianyou County Government of Putian, Fujian, China (Grant No. 2013B008); Cultivation Program for Distinguished Young Scholar of Fujian Province University (Grant No. 2017B020); and Fujian Medical University’s Research Foundation for Talented Scholars (Grant No. XRCZX2017012). All these funding did not involve in study design and collection, data analysis, and manuscript writing.

Disclosure

The authors report no conflicts of interest in this work.

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