Expression of transient receptor potential channel 6 in cervical cancer

Qi Wan,¹ Ai Zheng,¹ Xiaojing Liu,² Yali Chen,¹ Ling Han<sup>1

1</sup>Department of Obstetric and Gynecology, West China Second University Hospital, ²Laboratory of Cardiovascular Diseases, Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, China

Background: Recent studies have reported that aberrant expression of transient receptor potential channel C6 (TRPC6) in a variety of human cancers is associated with aggressive behavior. However, the functional significance of TRPC6 in human cervical cancer is not known. This study was planned to detect whether TRPC6 is expressed in cervical cancer tissue and to evaluate the association between TPRC6 expression and clinicopathologic features.
Methods: Tissue samples were collected from the West China Second UNIV Hospital of Sichuan University. TRPC6 expression was detected by quantitative real-time reverse transcription polymerase chain reaction and Western blotting. TRPC6 expression was evaluated by immunohistochemistry analysis of 40 cervical cancer specimens, and correlations were sought between elevated expression of TRPC6 and clinicopathologic features.
Results: Increased expression of TRPC6 was detected in 25 of the 40 cervical cancer samples. Positive cells found in cervical carcinomas were significantly increased in numbers compared with specimens without lymphovascular space invasion. Elevated expression of TRPC6 was neither related to International Federation of Gynecology and Obstetrics stage nor pelvic lymph metastases. Indeed, the clinicopathologic analysis indicated that overexpression of TRPC6 was significantly associated with lymphovascular space invasion.
Conclusion: These results indicate that elevated expression of TRPC6 might be associated with an aggressive cervical cancer phenotype.

Keywords: cervical cancer, transient receptor potential channel C6, overexpression, invasion