Back to Journals » International Journal of Nanomedicine » Volume 10 » Issue 1

Enhanced detection of single-cell-secreted proteins using a fluorescent immunoassay on the protein-G-terminated glass substrate

Authors Jeong Y, Lee KH, Park H, Choi J

Received 17 July 2015

Accepted for publication 29 October 2015

Published 24 November 2015 Volume 2015:10(1) Pages 7197—7205


Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Chang Liu

Peer reviewer comments 3

Editor who approved publication: Dr Thomas J Webster

Yoon Jeong,1,2 Kwan Hong Lee,1,2 Hansoo Park,3 Jonghoon Choi1,2

1Department of Bionano Technology, Graduate School, Hanyang University, Seoul, 2Department of Bionano Engineering, Hanyang University ERICA, Ansan, 3School of Integrative Engineering, Chung-Ang University, Seoul, South Korea

Abstract: We present an evaluation of protein-G-terminated glass slides that may contain a suitable substrate for aligning the orientation of antibodies to obtain better binding moiety to the target antigen. The results of the protein-G-terminated slides were compared with those obtained with epoxy-based slides to evaluate signal enhancement for human immunoglobulin G (IgG) targets, and an increase in the average fluorescence intensity was observed for the lowest measurable amount of IgG target in the assay using protein-G-terminated slides. Applying this strategy for signal amplification to single-cell assays improves the limits of detection for human IgG protein and cytokines (interleukin-2 and interferon-γ) captured from hybridomas. Our data indicate that protein-G-terminated slides have a higher binding capacity for antigens and have better spot-to-spot consistency than that of traditional epoxy-based slides. These properties would be beneficial in the detection of fine amounts of single-cell-secreted proteins, which may provide key insights into cell–cell communication and immune responses.

Keywords: microwell array, antibody’s orientation, single cell analysis, secreted cytokine, protein-G-terminated surface

Corrigendum for this paper has been published

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]


Other articles by this author:

Biomimetics: forecasting the future of science, engineering, and medicine

Hwang J, Jeong Y, Park JM, Lee KH, Hong JW, Choi J

International Journal of Nanomedicine 2015, 10:5701-5713

Published Date: 8 September 2015

Engineered collagen hydrogels for the sustained release of biomolecules and imaging agents: promoting the growth of human gingival cells

Choi J, Park H, Kim T, Jeong Y, Oh MH, Hyeon T, Gilad AA, Lee KH

International Journal of Nanomedicine 2014, 9:5189-5201

Published Date: 11 November 2014

Antibacterial activity and cytotoxicity of multi-walled carbon nanotubes decorated with silver nanoparticles

Seo Y, Hwang J, Kim J, Jeong Y, Hwang MP, Choi J

International Journal of Nanomedicine 2014, 9:4621-4629

Published Date: 30 September 2014

Readers of this article also read:

Development of polymeric–cationic peptide composite nanoparticles, a nanoparticle-in-nanoparticle system for controlled gene delivery

Jain AK, Massey A, Yusuf H, McDonald DM, McCarthy HO, Kett VL

International Journal of Nanomedicine 2015, 10:7183-7196

Published Date: 24 November 2015

mTOR signaling in the neuropathophysiology of depression: current evidence

Réus GZ, Quevedo J, Rodrigues AL

Journal of Receptor, Ligand and Channel Research 2015, 8:65-74

Published Date: 23 November 2015

Methacrylic-based nanogels for the pH-sensitive delivery of 5-Fluorouracil in the colon

Ashwanikumar N, Kumar NA, Nair SA, Kumar GS

International Journal of Nanomedicine 2012, 7:5769-5779

Published Date: 15 November 2012

Cross-linked acrylic hydrogel for the controlled delivery of hydrophobic drugs in cancer therapy

Deepa G, Thulasidasan AK, Anto RJ, Pillai JJ, Kumar GS

International Journal of Nanomedicine 2012, 7:4077-4088

Published Date: 27 July 2012

Crystallization after intravitreal ganciclovir injection

Pitipol Choopong, Nattaporn Tesavibul, Nattawut Rodanant

Clinical Ophthalmology 2010, 4:709-711

Published Date: 14 July 2010