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Electrochemical impedimetric biosensor based on a nanostructured polycarbonate substrate

Authors Chen YS, Wu CC, Tsai JJ, Wang GJ

Received 10 October 2011

Accepted for publication 30 November 2011

Published 6 January 2012 Volume 2012:7 Pages 133—140

DOI https://doi.org/10.2147/IJN.S27225

Review by Single-blind

Peer reviewer comments 2

Yu-Shan Chen1, Chia-Che Wu1, Jaw-Ji Tsai2, Gou-Jen Wang1,3
1Department of Mechanical Engineering, National Chung-Hsing University, 2Department of Medical Education and Research, Taichung Veterans General Hospital, 3Graduate Institute of Biomedical Engineering, National Chung-Hsing University, Taichung, Taiwan

Abstract: This study integrates the techniques of nanoelectroforming, hot-embossing, and electrochemical deposition to develop a disposable, low-cost, and high sensitivity nanostructure biosensor. A modified anodic aluminum oxide barrier-layer surface was used as the template for thin nickel film deposition. After etching the anodic aluminum oxide template off, a three-dimensional mold of the concave nanostructure array was created. The fabricated three-dimensional nickel mold was further used for replica molding of a nanostructure polycarbonate substrate by hot-embossing. A thin gold film was then sputtered onto the polycarbonate substrate to form the electrode, followed by deposition of an orderly and uniform gold nanoparticle layer on the three-dimensional gold electrode using electrochemical deposition. Finally, silver nanoparticles were deposited on the uniformly deposited gold nanoparticles to enhance the conductivity of the sensor. Electrochemical impedance spectroscopy analysis was then used to detect the concentration of the target element. The sensitivity of the proposed scheme on the detection of the dust mite antigen, Der p2, reached 0.1 pg/mL.

Keywords: nanoelectroforming, nanostructure polycarbonate substrate, gold nanoparticles, silver nanoparticles, electrochemical impedance spectroscopy

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