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Efficacy of retinol palmitate eye drops for dry eye in rabbits with lacrimal gland resection

Authors Odaka, Toshida H, Ohta T, Tabuchi, Koike, Suto C, Murakami A

Received 4 July 2012

Accepted for publication 9 August 2012

Published 1 October 2012 Volume 2012:6 Pages 1585—1593

DOI https://doi.org/10.2147/OPTH.S35584

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Akito Odaka,1 Hiroshi Toshida,2 Toshihiko Ohta,2 Nobuhito Tabuchi,3,4 Daisuke Koike,4 Chikako Suto,3 Akira Murakami3

1Human and Environmental Safety Evaluation Center, Lion Corporation, Kanagawa, Japan; 2Department of Ophthalmology, Juntendo University Shizuoka Hospital, Shizuoka, Japan; 3Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan; 4Pharmaceutical Research Laboratories No 1, Research and Development Headquarters, Lion Corporation, Kanagawa, Japan


Purpose: We examined the efficacy of retinol palmitate (VApal) for dry eyes using dry eye model rabbits whose lacrimal glands were resected.
Materials and methods: After alkaline injury on keratoconjunctival epithelium, VApal eye drops were administered 6 times a day for 7 days. The efficacy of VApal was also compared with that of 0.1% hyaluronic acid eye drops.
Results: The fluorescein staining and rose bengal scores showed a significant decrease compared with the score in the vehicle group at 7 days (P < 0.05) in the 1000 IU/mL VApal group and at both 3 days (P < 0.05) and 7 days (P < 0.01) in the 1500 IU/mL VApal group. Histological examination revealed recovery of the corneal epithelium, and PAS staining disclosed the recovery of mucin-producing lower palpebral conjunctival goblet cells after 7 days in the 1500 IU/mL VApal group compared with the vehicle group. Results from impression cytology showed a significant increase in density of conjunctival goblet cells compared with that in the vehicle group after 7 days in the 1000 IU/mL VApal group and after 3 and 7 days in the 1500 IU/mL VApal group. There were no significant changes in tear flow in either group. Topical application of VApal at 1500 IU/mL showed greater improvement than 0.1% hyaluronic acid in both fluorescein and rose bengal score and in the density of conjunctival goblet cells.
Conclusion: It is suggested that VApal is effective for the improvement of keratoconjunctival epithelial damage associated with tear abnormalities, such as dry eyes.

Keywords: cornea, dry eye, hyaluronic acid, vitamin A, wound healing, retinol palmitate

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