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Effects of Exposure to Mild Hyperbaric Oxygen on DSS-Induced Colonic Inflammation and Diarrhea in Rats

Authors Takemura A, Egawa T, Tanaka T, Kuramoto T, Hayashi T, Ishihara A

Received 24 June 2019

Accepted for publication 13 September 2019

Published 25 October 2019 Volume 2019:12 Pages 293—299

DOI https://doi.org/10.2147/JIR.S220586

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Melinda Thomas

Peer reviewer comments 2

Editor who approved publication: Dr Ning Quan


Ai Takemura,1 Tatsuro Egawa,2 Takuji Tanaka,3 Takashi Kuramoto,4 Tatsuya Hayashi,2 Akihiko Ishihara1

1Laboratory of Cell Biology and Life Science, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto 606-8501, Japan; 2Laboratory of Sports and Exercise Medicine, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto 606-8501, Japan; 3Department of Diagnostic Pathology, Gifu Municipal Hospital, Gifu 500-8513, Japan; 4Department of Animal Science, Faculty of Agriculture, Tokyo University of Agriculture, Atsugi 243-0034, Japan

Correspondence: Ai Takemura
Department of Sports Research, Japan Institute of Sport Sciences, Tokyo 115-0056, Japan
Tel +81-75-753-2996
Fax +81-75-753-6771
Email takemura.ai.46c@kyoto-u.jp

Purpose: In rodents, dextran sulfate sodium (DSS)-induced diarrhea and colonic inflammation have similar symptoms to those of ulcerative colitis in humans. We examined the effects of exposure to mild hyperbaric oxygen (MHO) at an atmospheric pressure of 1317 hPa with 40% oxygen on DSS-induced diarrhea and colonic inflammation in rats.
Methods: Five-week-old male Kyoto Apc Delta (KAD) rats (n = 12) were administered 2% DSS through drinking water for 1 week. Subsequently, DSS-treated male rats were not subjected to any further treatment (n = 6) or exposed to MHO (n = 6) for 2 weeks. Age-matched KAD rats not subjected to DSS treatment or exposed to MHO were used as the control group (n = 6).
Results: Control rats did not exhibit diarrhea and colonic inflammation. However, DSS-treated rats exhibited diarrhea and colonic inflammation, regardless of exposure to MHO. Exposure to MHO for 2 weeks led to decreased incidence of diarrhea in DSS-treated rats (p < 0.05). Exposure to MHO had no effect on colonic inflammation in DSS-treated rats (p = 0.12).
Conclusion: Exposure to MHO for 2 weeks can improve diarrhea but cannot attenuate colonic inflammation, possibly due to the short exposure duration (2 weeks) used in this study.

Keywords: colonic inflammation, dextran sulfate sodium, Kyoto Apc Delta rats, mild hyperbaric oxygen, oxidative metabolism

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