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Effect of liposomal fluidity on skin permeation of sodium fluorescein entrapped in liposomes

Authors Subongkot T, Ngawhirunpat T

Received 15 April 2015

Accepted for publication 16 May 2015

Published 17 July 2015 Volume 2015:10(1) Pages 4581—4592

DOI https://doi.org/10.2147/IJN.S86624

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Editor who approved publication: Professor Carlos Rinaldi


Thirapit Subongkot,1 Tanasait Ngawhirunpat2

1
Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Burapha University, Chonburi, Thailand; 2Department of Pharmaceutical Technology, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, Thailand

Abstract: The purpose of this study was to investigate the effect of ultradeformable liposome components, Tween 20 and terpenes, on vesicle fluidity. The fluidity was evaluated by electron spin resonance spectroscopy using 5-doxyl stearic acid and 16-doxyl stearic acid as spin labels for phospholipid bilayer fluidity at the C5 atom of the acyl chain near the polar head group (hydrophilic region) and the C16 atom of the acyl chain (lipophilic region), respectively. The electron spin resonance study revealed that Tween 20 increased the fluidity at the C5 atom of the acyl chain, whereas terpenes increased the fluidity at the C16 atom of the acyl chain of the phospholipid bilayer. The increase in liposomal fluidity resulted in the increased skin penetration of sodium fluorescein. Confocal laser scanning microscopy showed that ultradeformable liposomes with terpenes increase the skin penetration of sodium fluorescein by enhancing hair follicle penetration.

Keywords: ultradeformable liposomes, terpenes, fluidity, electron spin resonance spectroscopy, confocal laser scanning microscopy

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