Back to Journals » Oncolytic Virotherapy » Volume 8

Directed evolution as a tool for the selection of oncolytic RNA viruses with desired phenotypes

Authors Zainutdinov SS, Kochneva GV, Netesov SV, Chumakov PM, Matveeva OV

Received 3 March 2019

Accepted for publication 7 June 2019

Published 12 July 2019 Volume 2019:8 Pages 9—26


Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Tommy Alain

Sergei S Zainutdinov,1 Galina V Kochneva,1 Sergei V Netesov,2 Peter M Chumakov3,4, Olga V Matveeva5

1State Research Center of Virology and Biotechnology “Vector”, Koltsovo 630559, Russia; 2Department of Natural Sciences, Novosibirsk State University, Novosibirsk 630090, Russia; 3Engelhardt Institute of Molecular Biology, Moscow 119991, Russia; 4Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products, Moscow 108819, Russia; 5SATOR Therapeutics LLC, Cleveland, OH 44106, USA

Abstract: Viruses have some characteristics in common with cell-based life. They can evolve and adapt to environmental conditions. Directed evolution can be used by researchers to produce viral strains with desirable phenotypes. Through bioselection, improved strains of oncolytic viruses can be obtained that have better safety profiles, increased specificity for malignant cells, and more efficient spread among tumor cells. It is also possible to select strains capable of killing a broader spectrum of cancer cell variants, so as to achieve a higher frequency of therapeutic responses. This review describes and analyses virus adaptation studies performed with members of four RNA virus families that are used for viral oncolysis: reoviruses, paramyxoviruses, enteroviruses, and rhabdoviruses.

Keywords: oncolytic viruses, virus selection, virus adaptation, directed viral evolution

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]