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Directed evolution as a tool for the selection of oncolytic RNA viruses with desired phenotypes

Authors Zainutdinov SS, Kochneva GV, Netesov SV, Chumakov PM, Matveeva OV

Received 3 March 2019

Accepted for publication 7 June 2019

Published 12 July 2019 Volume 2019:8 Pages 9—26

DOI https://doi.org/10.2147/OV.S176523

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Cristina Weinberg

Peer reviewer comments 2

Editor who approved publication: Dr Tommy Alain


Sergei S Zainutdinov,1 Galina V Kochneva,1 Sergei V Netesov,2 Peter M Chumakov3,4, Olga V Matveeva5

1State Research Center of Virology and Biotechnology “Vector”, Koltsovo 630559, Russia; 2Department of Natural Sciences, Novosibirsk State University, Novosibirsk 630090, Russia; 3Engelhardt Institute of Molecular Biology, Moscow 119991, Russia; 4Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products, Moscow 108819, Russia; 5SATOR Therapeutics LLC, Cleveland, OH 44106, USA

Abstract: Viruses have some characteristics in common with cell-based life. They can evolve and adapt to environmental conditions. Directed evolution can be used by researchers to produce viral strains with desirable phenotypes. Through bioselection, improved strains of oncolytic viruses can be obtained that have better safety profiles, increased specificity for malignant cells, and more efficient spread among tumor cells. It is also possible to select strains capable of killing a broader spectrum of cancer cell variants, so as to achieve a higher frequency of therapeutic responses. This review describes and analyses virus adaptation studies performed with members of four RNA virus families that are used for viral oncolysis: reoviruses, paramyxoviruses, enteroviruses, and rhabdoviruses.

Keywords: oncolytic viruses, virus selection, virus adaptation, directed viral evolution

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