Back to Journals » International Journal of Nanomedicine » Volume 10 » Issue 1

Delivery of vincristine sulfate-conjugated gold nanoparticles using liposomes: a light-responsive nanocarrier with enhanced antitumor efficiency

Authors Liu Y, He M, Niu M, Zhao Y, Zhu Y, Li Z, Feng N

Received 18 December 2014

Accepted for publication 31 January 2015

Published 22 April 2015 Volume 2015:10(1) Pages 3081—3095

DOI https://doi.org/10.2147/IJN.S79550

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 4

Editor who approved publication: Dr Thomas J Webster

Ying Liu,1,* Man He,1,* Mengmeng Niu,1 Yiqing Zhao,1 Yuanzhang Zhu,1 Zhenhua Li,2 Nianping Feng1

1Department of Pharmaceutical Sciences, School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China; 2Cedars-Sinai Medical Center, Los Angeles, CA, USA

*These authors contributed equally to this work

Abstract: Rapid drug release at the specific site of action is still a challenge for antitumor therapy. Development of stimuli-responsive hybrid nanocarriers provides a promising strategy to enhance therapeutic effects by combining the unique features of each component. The present study explored the use of drug–gold nanoparticle conjugates incorporated into liposomes to enhance antitumor efficiency. A model drug, vincristine sulfate, was physically conjugated with gold nanoparticles and verified by UV-visible and fourier transform infrared spectroscopy, and differential scanning calorimetry. The conjugates were incorporated into liposomes by film dispersion to yield nanoparticles (113.4 nm) with light-responsive release properties, as shown by in vitro release studies. Intracellular uptake and distribution was studied in HeLa cells using transmission electron microscopy and confocal laser scanning microscopy. This demonstrated liposome internalization and localization in endosomal–lysosomal vesicles. Fluorescence intensity increased in cells exposed to UV light, indicating that this stimulated intracellular drug release; this finding was confirmed by quantitative analyses using flow cytometry. Antitumor efficacy was evaluated in HeLa cells, both in culture and in implants in vivo in nude mice. HeLa cell viability assays showed that light exposure enhanced liposome cytotoxicity and induction of apoptosis. Furthermore, treatment with the prepared liposomes coupled with UV light exposure produced greater antitumor effects in nude mice and reduced side effects, as compared with free vincristine sulfate.

Keywords: vincristine sulfate, vincristine sulfate–gold nanoparticle conjugates, liposomes, antitumor efficiency, light triggered drug release
 

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]

 

Other articles by this author:

Preparation and evaluation of microemulsion-based transdermal delivery of total flavone of rhizoma arisaematis

Shen LN, Zhang YT, Wang Q, Xu L, Feng NP

International Journal of Nanomedicine 2014, 9:3453-3464

Published Date: 22 July 2014

In vitro cellular uptake of evodiamine and rutaecarpine using a microemulsion

Zhang YT, Huang ZB, Zhang SJ, Zhao JH, Wang Z, Liu Y, Feng NP

International Journal of Nanomedicine 2012, 7:2465-2472

Published Date: 18 May 2012

Preparation and characterization of solid lipid nanoparticles loaded with frankincense and myrrh oil

Shi F, Zhao JH, Liu Y, Wang Z, Zhang YT, Feng NP

International Journal of Nanomedicine 2012, 7:2033-2043

Published Date: 17 April 2012

Corrigendum

Chen ZQ, Liu Y, Zhao JH, Wang L, Feng NP

International Journal of Nanomedicine 2012, 7:1709-1710

Published Date: 30 March 2012

RGD-modified poly(D,L-lactic acid) nanoparticles enhance tumor targeting of oridonin

Xu J, Zhao JH, Liu Y, Feng NP, Zhang YT

International Journal of Nanomedicine 2012, 7:211-219

Published Date: 9 January 2012

Enhanced transdermal delivery of evodiamine and rutaecarpine using microemulsion

Zhong YT, Zhao JH, Zhang SJ, Zhong YZ, Wang Z, Liu Y, Shi F, Feng NP

International Journal of Nanomedicine 2011, 6:2469-2482

Published Date: 21 October 2011

Microemulsion-based novel transdermal delivery system of tetramethylpyrazine: preparation and evaluation in vitro and in vivo

Zhao JH, Ji L, Wang H, Chen ZQ, Zhang YT, Liu Y, Feng NP

International Journal of Nanomedicine 2011, 6:1611-1619

Published Date: 9 August 2011

Readers of this article also read:

Green synthesis of water-soluble nontoxic polymeric nanocomposites containing silver nanoparticles

Prozorova GF, Pozdnyakov AS, Kuznetsova NP, Korzhova SA, Emel’yanov AI, Ermakova TG, Fadeeva TV, Sosedova LM

International Journal of Nanomedicine 2014, 9:1883-1889

Published Date: 16 April 2014

Methacrylic-based nanogels for the pH-sensitive delivery of 5-Fluorouracil in the colon

Ashwanikumar N, Kumar NA, Nair SA, Kumar GS

International Journal of Nanomedicine 2012, 7:5769-5779

Published Date: 15 November 2012

Cross-linked acrylic hydrogel for the controlled delivery of hydrophobic drugs in cancer therapy

Deepa G, Thulasidasan AK, Anto RJ, Pillai JJ, Kumar GS

International Journal of Nanomedicine 2012, 7:4077-4088

Published Date: 27 July 2012

Crystallization after intravitreal ganciclovir injection

Pitipol Choopong, Nattaporn Tesavibul, Nattawut Rodanant

Clinical Ophthalmology 2010, 4:709-711

Published Date: 14 July 2010