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Zafirlukast in Combination with Pseudohypericin Attenuates Spinal Cord Injury and Motor Function in Experimental Mice [Corrigendum]

Authors Chen XG, Hua F, Wang SG, Xu YY, Yue HT, Sun J

Received 28 January 2021

Accepted for publication 28 January 2021

Published 9 February 2021 Volume 2021:15 Pages 419—422

DOI https://doi.org/10.2147/DDDT.S304214


Chen XG, Hua F, Wang SG, Xu YY, Yue HT, Sun J. Drug Des Devel Ther. 2018;12:2389–2402

The authors have advised Figures 1 (on page 2393), Figure 3 (on page 2394), Figure 5 (on page 2395), Figure 7 and Figure 8 (on page 2396) are incorrect due to a fault in their microscope camera. The correct Figures are shown below:

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Chen XG, Hua F, Wang SG, Xu YY, Yue HT, Sun J. Drug Des Devel Ther. 2018;12:2389–2402

The authors have advised Figures 1 (on page 2393), Figure 3 (on page 2394), Figure 5 (on page 2395), Figure 7 and Figure 8 (on page 2396) are incorrect due to a fault in their microscope camera. The correct Figures are shown below:

Figure 1 Effect of PHP and ZFL on histological changes on the spinal cord tissue after 24 h inducing spinal injury.

Abbreviations: PHP, pseudohypericin; ZFL, zafirlukast; wm, white matter, gm, grey matter.

Notes: (A) Vehicle-treated control group showing occurrence of edema and changes in white matter of perilesional zone. (B) Sham-vehicle treated group mice showing no signs of changes. The severity of spinal cord injury decreased in tissues with reduction in edema and preservation of white matter observed in sections (C) PHP and (D) ZFL treated mice. The results were improved in (E) after combining PHP and ZFL. The produced figures are representatives of 3 experiments.

Figure 3 Immunohistochemical localization and activity of MPO in spinal tissue of mice treated with PHP and ZFL their combination.

Abbreviations: MPO, myeloperoxidase; PHP, pseudohypericin; ZFL, zafirlukast; relative standard deviation.

Notes: In vehicle-treated control group mice, the tissue were stained positive for MPO and presented increased MPO activity (A and F). Sham-operated group mice, tissue sections showed no positive staining for MPO and also had normalized MPO activity (B and F). MPO staining in tissues sections from PHP (C), ZFL (D) and their combination (E) a significant reduction in positive staining for MPO activity was seen compared to vehicle treated control group. The produced figures are representatives of 3 experiments. Data are mean ± %RSD (n=10), *P<0.001 compared to control vehicle treated mice.

Figure 5 Effect of PHP and ZFL on tissue levels and immunohistochemical localization of TNF-α.

Abbreviations: PHP, pseudohypericin; ZFL, zafirlukast; TNF-α, tumor necrosis factor-α; wm, white matter, gm, grey matter.

Notes: The control vehicle treated group showed increased levels of TNF-α (F) and positive staining in spinal tissues (A). (B) Sham-group do not exhibited staining. The treatment of PHP, ZFL and their combination significantly (*P<0.01) reduced levels of TNF-α compared to control (F), also reduced the positive staining in spinal tissues (C, D and E respectively). The results were highly significant (#P<0.001) in mice treated with combination and sham group compared to control.

Figure 7 TUNEL assay of spinal cord tissue of mice subjected to spinal injury followed by treatment of PHP, ZFL and their combination.

Abbreviations: PHP, pseudohypericin; ZFL, zafirlukast; TUNEL, terminal deoxynucleotidyltransferase-mediated UTP end labeling.

Notes: The tissue harvested after 24 h of injury and received treatment of vehicle only showed appearance of brown color cells suggesting apoptosis (A). In contrast the tissues of mice treated with PHP (C), ZFL (D) and their combination (E) demonstrated reduction in number of apoptotic cells, no apoptotic fragments were detected in sham-operated vehicle treated mice spinal tissues (B). TUNEL- terminal deoxynucleotidyltransferase-mediated UTP end labelling assay. The arrows indicate the TUNEL positive cells.

Figure 8 Results of immunohistological staining for FAS-L.

Abbreviations: PHP, pseudohypericin; ZFL, zafirlukast; FAS-L, FAS ligand; wm, white matter; gm, gray matter.

Notes: The spinal tissues sections obtained after 24 h of spinal injury showed positive staining for FAS-Ligand (A). The spinal tissue sections of mice after receiving treatment of PHP (C) and ZFL (D) showed decrease in number of cells positive for FAS-L. The positive stained FAS-L cells reduced more significantly in spinal tissues of mice receiving combined treatment of PHP and ZFL (E). The sham-operated mice tissues showed no staining for FAS-L (B).

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