Back to Journals » Clinical Interventions in Aging » Volume 11

Correlation between vitamin D levels and apoptosis in geriatric patients infected with hepatitis C virus genotype 4

Authors Gabr SA, Alghadir AH, Allam AA, Ajarem J, Al-Basher G, Abdel-Maksoud MA, Ghfar AA, Aboud A

Received 19 January 2016

Accepted for publication 2 March 2016

Published 4 May 2016 Volume 2016:11 Pages 523—533

DOI https://doi.org/10.2147/CIA.S104599

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Supriya Swarnkar

Peer reviewer comments 3

Editor who approved publication: Dr Richard Walker

Sami A Gabr,1,2 Ahmad H Alghadir,1 Ahmed A Allam,3,4 Jamaan Ajarem,3 Ghada Al-Basher,3 Mostafa A Abdel-Maksoud,3 Ayman A Ghfar,5 Alaa Aboud6

1Rehabilitation Research Chair, College of Applied Medical Sciences, King Saud University, Riyadh, Kingdom of Saudi Arabia; 2Department of Anatomy, Faculty of Medicine, Mansoura University, Mansoura, Egypt; 3Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia; 4Zoology Department, Faculty of Science, Beni-Suef University, Beni Suef, Egypt; 5Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia; 6Internal Endemic Medicine Department, College of Medicine, Beni-Suef University, Beni Suef, Egypt

Background: Vitamin D levels play a pivotal role in most biological processes and differ according to age. A deficiency of vitamin D in chronic hepatitis C (CHC) patients has been shown to be linked with the severity of liver fibrosis, but little is known about the mechanism of this association.
Objective: In this study, we evaluate the potential interrelation between vitamin D levels, oxidative stress, and apoptosis, based on liver fibrosis in geriatric patients infected with hepatitis C virus (HCV) genotype 4.
Subjects and methods: A total of 120 adult individuals aged 30–68 years were recruited in this study. Of these, 20 healthy subjects (15 men and five women) with a mean age of 48.3±6.1 years were selected as controls, and 100 patients with a mean age of 47.8±4.9 years with chronic HCV (CHC) who had undergone liver biopsy (80 men and 20 women) were included in this study. Based on liver radiographic (computed tomography, magnetic resonance imaging) and histological Metavir system analyses, the CHC patients were classified into three groups: asymptomatic CHC carriers (n=30), fibrosis (n=25), and cirrhosis (n=45). HCV RNA, HCV genotypes, inflammatory cytokines AFP and TNFα, 25-hydroxyvitamin D (25[OH]D) levels, apoptotic markers single-stranded DNA (ssDNA) and soluble Fas (sFas), and oxidative stress markers nitric oxide (NO) and total antioxidant capacity (TAC) were estimated by using molecular, immunoassay, and colorimetric techniques.
Results:
Approximately 30% of the study population (n=30) were diagnosed as asymptomatic CHC carriers, and 70% of the study population (n=70) had severe fibrosis; these were classified into fibrosis and cirrhosis. There was a significant reduction in 25(OH)D levels and TAC activity, along with an increase in levels of NO, AFP, TNFα, ssDNA, and sFas in fibrosis and cirrhosis subjects compared with those of asymptomatic CHC carriers and health controls. The deficiency in 25(OH)D levels correlated positively with sFas, ssDNA, AFP, TNFα, NO, and TAC, and negatively with age, sex, liver function, body mass index, homeostatic model assessment – insulin resistance, HCV RNA, and viral load. Significant intercorrelation was reported between serum 25(OH)D concentrations and apoptotic and oxidative markers, which suggested progression of liver pathogenesis and fibrogenesis via oxidative and apoptotic mechanisms.
Conclusion: The data showed that vitamin D status was significantly correlated with pathogenesis and fibrogenesis of the liver in geriatric patients infected with HCV genotype 4. The deficiency in 25(OH)D levels was shown to have a pivotal role in the pathogenesis of liver via apoptotic, oxidative stress, and inflammatory mechanistic pathways. The data point to adequate vitamin D levels being recommended for a good response to treatment strategies, especially in older CHC patients.

Keywords: 25(OH)D, HCV, apoptosis, Fas antigen, liver fibrosis, oxidative stress, geriatrics

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]

 

Other articles by this author:

Factors related to gait and balance deficits in older adults

Al-Momani M, Al-Momani F, Alghadir AH, Alharethy S, Gabr SA

Clinical Interventions in Aging 2016, 11:1043-1049

Published Date: 9 August 2016

Correlation between bone mineral density and serum trace elements in response to supervised aerobic training in older adults

Alghadir AH, Gabr SA, Al-Eisa ES, Alghadir MH

Clinical Interventions in Aging 2016, 11:265-273

Published Date: 29 February 2016

Readers of this article also read:

Clinical assessment tools identify functional deficits in fragility fracture patients

Ames TD, Wee CE, Le KM, Wang TL, Bishop JY, Phieffer LS, Quatman CE

Clinical Interventions in Aging 2016, 11:563-570

Published Date: 5 May 2016

Frailty and quality of life in elderly patients with acute coronary syndrome

Lisiak M, Uchmanowicz I, Wontor R

Clinical Interventions in Aging 2016, 11:553-562

Published Date: 5 May 2016

Immediate results and long-term cardiovascular outcomes of endovascular therapy in octogenarians and nonoctogenarians with peripheral arterial diseases

Huang HL, Jimmy Juang JM, Chou HH, Hsieh CA, Jang SJ, Cheng ST, Ko YL

Clinical Interventions in Aging 2016, 11:535-543

Published Date: 4 May 2016

Design, synthesis, and biological evaluation of 3-vinyl-quinoxalin-2(1H)-one derivatives as novel antitumor inhibitors of FGFR1

Liu Z, Yu S, Chen D, Shen G, Wang Y, Hou L, Lin D, Zhang JS, Ye F

Drug Design, Development and Therapy 2016, 10:1489-1500

Published Date: 3 May 2016

Hospitalization in older patients due to adverse drug reactions – the need for a prediction tool

Parameswaran Nair N, Chalmers L, Peterson GM, Bereznicki BJ, Castelino RL, Bereznicki LR

Clinical Interventions in Aging 2016, 11:497-505

Published Date: 2 May 2016