Back to Journals » International Journal of Nanomedicine » Volume 8 » Issue 1

Comparative proteomics of inhaled silver nanoparticles in healthy and allergen provoked mice

Authors Su C, Chen T, Chang C, Chuang K, Cheng-Kuan Wu, Liu W, Ho K, Lee K, Ho S, Tseng H, Chuang H, Cheng T

Received 20 April 2013

Accepted for publication 24 May 2013

Published 2 August 2013 Volume 2013:8(1) Pages 2783—2799


Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Chien-Ling Su,1,2 Tzu-Tao Chen,1,3 Chih-Cheng Chang,1,3 Kai-Jen Chuang,4,5 Cheng-Kuan Wu,6 Wen-Te Liu,1,2 Kin Fai Ho,7 Kang-Yun Lee,1,8 Shu-Chuan Ho,2,8 Hsiu-Er Tseng,9 Hsiao-Chi Chuang,1,2 Tsun-Jen Cheng6,10

On behalf of the Taiwan CardioPulmonary Research Group (T-CPR)

1Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho Hospital, 2School of Respiratory Therapy, College of Medicine, 3Graduate Institute of Clinical Medicine, College of Medicine, 4Department of Public Health, School of Medicine, College of Medicine, 5School of Public Health, College of Public Health and Nutrition, Taipei Medical University, 6Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University, Taipei, Taiwan; 7School of Public Health and Primary Care, The Chinese University of Hong Kong, Hong Kong, People's Republic of China; 8Department of Thoracic Medicine, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, 9Division of Consultation and Promotion, Taiwan Drug Relief Foundation, 10Department of Public Health, College of Public Health, National Taiwan University, Taipei, Taiwan

Background: Silver nanoparticles (AgNPs) have been associated with the exacerbation of asthma; however, the immunological basis for the adjuvant effects of AgNPs is not well understood.
Objective: The aim of the study reported here was to investigate the allergic effects of AgNP inhalation using proteomic approaches.
Methods: Allergen provoked mice were exposed to 33 nm AgNPs at 3.3 mg/m3. Following this, bronchoalveolar lavage fluid (BALF) and plasma were collected to determine protein profiles.
Results: In total, 106 and 79 AgNP-unique proteins were identified in the BALF of control and allergic mice, respectively. Additionally, 40 and 26 AgNP-unique proteins were found in the plasma of control and allergic mice, respectively. The BALF and plasma protein profiles suggested that metabolic, cellular, and immune system processes were associated with pulmonary exposure to AgNPs. In addition, we observed 18 proteins associated with systemic lupus erythematosus that were commonly expressed in both control and allergic mice after AgNP exposure. Significant allergy responses were observed after AgNP exposure in control and allergic mice, as determined by ovalbumin-specific immunoglobulin E.
Conclusion: Inhaled AgNPs may regulate immune responses in the lungs of both control and allergic mice. Our results suggest that immunology is a vital response to AgNPs.

Keywords: bronchoalveolar lavage, immunotoxicology, proteome, systemic lupus erythematosus, serum

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]


Other articles by this author:

Associations of autophagy with lung diffusion capacity and oxygen saturation in severe COPD: effects of particulate air pollution

Lee KY, Chiang LL, Ho SC, Liu WT, Chen TT, Feng PH, Su CL, Chuang KJ, Chang CC, Chuang HC

International Journal of Chronic Obstructive Pulmonary Disease 2016, 11:1569-1578

Published Date: 11 July 2016

Particulate matter is associated with sputum culture conversion in patients with culture-positive tuberculosis

Chen KY, Chuang KJ, Liu HC, Lee KY, Feng PH, Su CL, Lin CL, Lee CN, Chuang HC

Therapeutics and Clinical Risk Management 2016, 12:41-46

Published Date: 6 January 2016

Cigarette smoke is a risk factor for severity and treatment outcome in patients with culture-positive tuberculosis

Chuang HC, Su CL, Liu HC, Feng PH, Lee KY, Chuang KJ, Lee CN, Bien MY

Therapeutics and Clinical Risk Management 2015, 11:1539-1544

Published Date: 6 October 2015

Characterization of pulmonary protein profiles in response to zinc oxide nanoparticles in mice: a 24-hour and 28-day follow-up study

Pan CH, Chuang KJ, Chen JK, Hsiao TC, Lai CH, Jones TP, BéruBé KA, Hong GB, Ho KF, Chuang HC

International Journal of Nanomedicine 2015, 10:4705-4716

Published Date: 27 July 2015

Effects of size and surface of zinc oxide and aluminum-doped zinc oxide nanoparticles on cell viability inferred by proteomic analyses

Pan CH, Liu WT, Bien MY, Lin IC, Hsiao TC, Ma CM, Lai CH, Chen MC, Chuang KJ, Chuang HC

International Journal of Nanomedicine 2014, 9:3631-3643

Published Date: 2 August 2014

Readers of this article also read:

Emerging and future therapies for hemophilia

Carr ME, Tortella BJ

Journal of Blood Medicine 2015, 6:245-255

Published Date: 3 September 2015

A new recombinant factor VIII: from genetics to clinical use

Kannicht C, Kohla G, Tiemeyer M, Walter O, Sandberg H

Drug Design, Development and Therapy 2015, 9:3817-3819

Published Date: 23 July 2015

Second case report of successful electroconvulsive therapy for a patient with schizophrenia and severe hemophilia A

Saito N, Shioda K, Nisijima K, Kobayashi T, Kato S

Neuropsychiatric Disease and Treatment 2014, 10:865-867

Published Date: 16 May 2014

A novel preparation method for silicone oil nanoemulsions and its application for coating hair with silicone

Hu Z, Liao M, Chen Y, Cai Y, Meng L, Liu Y, Lv N, Liu Z, Yuan W

International Journal of Nanomedicine 2012, 7:5719-5724

Published Date: 12 November 2012

Cross-linked acrylic hydrogel for the controlled delivery of hydrophobic drugs in cancer therapy

Deepa G, Thulasidasan AK, Anto RJ, Pillai JJ, Kumar GS

International Journal of Nanomedicine 2012, 7:4077-4088

Published Date: 27 July 2012

The use of PEGylated liposomes in the development of drug delivery applications for the treatment of hemophilia

Rivka Yatuv, Micah Robinson, Inbal Dayan-Tarshish, et al

International Journal of Nanomedicine 2010, 5:581-591

Published Date: 6 August 2010