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Cellular uptake and fate of fibroin microspheres loaded with randomly fragmented DNA in 3T3 cells

Authors Lee JS, Hur W

Received 7 January 2016

Accepted for publication 12 March 2016

Published 13 May 2016 Volume 2016:11 Pages 2069—2079


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 4

Editor who approved publication: Dr Thomas Webster

Supplementary video presented by Lee and Hur.

Views: 230

Jin Sil Lee, Won Hur

Department of Bioengineering and Technology, Kangwon National University, Chuncheon, South Korea

Abstract: Purified fibroin protein can be obtained in large quantities from silk fibers and processed to form microscopic particles as delivery vehicles for therapeutic agents. In this study, we demonstrated that fibroin microspheres were taken up by 3T3 cells, localized in the nonlysosomal compartment, and secreted from the cytoplasm after medium replenishment. DNA-loaded microspheres were taken up by >95% of 3T3 cells. DNA cargo had no influence on the intracellular trafficking of microspheres, while fluorescently labeled cargo DNA was observed in the lysosomal compartment and in the microspheres. These results indicate that fibroin microspheres can travel through 3T3 cells without making any contact with the lysosomal compartments. The amount of DNA loaded in the microspheres taken up by 3T3 cells was estimated up to 831.0 pg/cell. Thus, fibroin microspheres can deliver a large amount of randomly fragmented DNA (<10 kb) into the cytoplasmic compartment of 3T3 cells.

Keywords: microspheres, DNA delivery, transcellular transport, fibroin

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