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Cellular interaction of a layer-by-layer based drug delivery system depending on material properties and cell types

Authors Brueckner M, Jankuhn S, Jülke EM, Reibetanz U

Received 10 October 2017

Accepted for publication 9 December 2017

Published 5 April 2018 Volume 2018:13 Pages 2079—2091

DOI https://doi.org/10.2147/IJN.S153701

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Govarthanan Muthusamy

Peer reviewer comments 3

Editor who approved publication: Dr Thomas J Webster


Mandy Brueckner,1 Steffen Jankuhn,2,3 Eva-Maria Jülke,1 Uta Reibetanz1

1Institute for Medical Physics and Biophysics, Medical Faculty, University of Leipzig, Leipzig, Germany; 2Division of Nuclear Solid State Physics, Faculty of Physics and Geosciences, University of Leipzig, Leipzig, Germany; 3Office for Environmental Protection and Occupational Safety, University of Leipzig, Leipzig, Germany

Background: Drug delivery systems (DDS) and their interaction with cells are a controversial topic in the development of therapeutic concepts and approaches. On one hand, DDS are very useful for protected and targeted transport of defined dosages of active agents. On the other hand, their physicochemical properties such as material, size, shape, charge, or stiffness have a huge impact on cellular uptake and intracellular processing. Additionally, even identical DDS can undergo a completely diverse interaction with different cell types. However, quite often in in vitro DDS/cell interaction experiments, those aspects are not considered and DDS and cells are randomly chosen.
Methods and results: Hence, our investigations provide an insight into layer-by-layer designed microcarriers with modifications of only some of the most important parameters (surface charge, stiffness, and applied microcarrier/cell ratio) and their influence on cellular uptake and viability. We also considered the interaction of these differently equipped DDS with several cell types and investigated professional phagocytes (neutrophil granulocytes; macrophages) as well as non-professional phagocytes (epithelial cells) under comparable conditions. We found that even small modifications such as layer-by-layer (LbL)-microcarriers with positive or negative surface charge, or LbL-microcarriers with solid core or as hollow capsules but equipped with the same surface properties, show significant differences in interaction and viability, and several cell types react very differently to the offered DDS.
Conclusion: As a consequence, the properties of the DDS have to be carefully chosen with respect to the addressed cell type with the aim to efficiently transport a desired agent.

Keywords: layer-by-layer, LbL, uptake, cell viability, microparticles, microcapsules, material properties

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