Back to Journals » International Journal of Nanomedicine » Volume 8 » Issue 1

Catalytic nanomedicine technology: copper complexes loaded on titania nanomaterials as cytotoxic agents of cancer cell

Authors Lopez T, Ortiz-Islas E, Guevara P, Gómez E

Received 17 August 2012

Accepted for publication 27 October 2012

Published 9 February 2013 Volume 2013:8(1) Pages 581—592


Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Tessy Lopez,1–3 Emma Ortiz-Islas,2 Patricia Guevara,4 Esteban Gómez2

1Nanotechnology and Nanomedicine Laboratory, Metropolitan Autonomous University-Xochimilco, Coyoacán, México; 2Nanotechnology Laboratory, National Institute of Neurology and Neurosurgery "MVS," Tlalpan, México; 3Department of Chemical and Biomolecular Engineering, Tulane University, New Orleans, LA, USA; 4Neuroimmunology Laboratory, National Institute of Neurology and Neurosurgery "MVS," Tlalpan, México

Abstract: The anticancer properties of pure copper (II) acetate and copper (II) acetylacetonate, alone and loaded on functionalized sol-gel titania (TiO2), were determined in four different cancer cell lines (C6, RG2, B16, and U373), using increasing concentrations of these compounds. The copper complexes were loaded onto the TiO2 network during its preparation by the sol-gel process. Once copper-TiO2 materials were obtained, these were characterized by several physical-chemical techniques. An in vitro copper complex–release test was developed in an aqueous medium at room temperature and monitored by ultraviolet spectroscopy. The toxic effect of the copper complexes, alone and loaded on TiO2, was determined using a cell viability 3(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay, when cancer cells were treated with increasing concentrations (15.75–1000 mg/mL) of these. Characterization studies revealed that the addition of copper complexes to the TiO2 sol-gel network during its preparation, did not generate changes in the molecular structure of the complexes. The surface area, pore volume, and pore diameter were affected by the copper complex additions and by the crystalline phases obtained. The kinetic profiles of both copper complexes released indicated two different stages of release: The first one was governed by first-order kinetics and the second was governed by zero-order kinetics. The cell viability assay revealed a cytotoxic effect of copper complexes, copper-TiO2, and cisplatin in a dose-dependent response for all the cell lines; however, the copper complexes exhibited a better cytotoxic effect than the cisplatin compound. TiO2 alone presented a minor cytotoxicity for C6 and B16 cells; however, it did not cause any toxic effect on the RG2 and U373 cells, which indicates its high biocompatibility with these cells.

Keywords: functionalized-TiO2, anticancer properties, toxic effect, cancer cell lines

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]


Readers of this article also read:

Gold nanoparticles as physiological markers of urine internalization into urothelial cells in vivo

Hudoklin S, Zupančič D, Makovec D, Kreft ME, Romih R

International Journal of Nanomedicine 2013, 8:3945-3953

Published Date: 14 October 2013

Process optimization and evaluation of novel baicalin solid nanocrystals

Yue PF, Li Y, Wan J, Wang Y, Yang M, Zhu WF, Wang CH, Yuan HL

International Journal of Nanomedicine 2013, 8:2961-2973

Published Date: 9 August 2013

Multilayer bioactive glass/zirconium titanate thin films in bone tissue engineering and regenerative dentistry

Mozafari M, Salahinejad E, Shabafrooz V, Yazdimamaghani M, Vashaee D, Tayebi L

International Journal of Nanomedicine 2013, 8:1665-1672

Published Date: 26 April 2013

Characterization of cellular uptake and toxicity of aminosilane-coated iron oxide nanoparticles with different charges in central nervous system-relevant cell culture models

Sun Z, Yathindranath V, Worden M, Thliveris JA, Chu S, Parkinson FE, Hegmann T, Miller DW

International Journal of Nanomedicine 2013, 8:961-970

Published Date: 7 March 2013

Application of magnetically induced hyperthermia in the model protozoan Crithidia fasciculata as a potential therapy against parasitic infections

Grazú V, Silber AM, Moros M, Asín L, Torres TE, Marquina C, Ibarra MR, Goya GF

International Journal of Nanomedicine 2012, 7:5351-5360

Published Date: 8 October 2012

The scent fingerprint of hepatocarcinoma: in-vitro metastasis prediction with volatile organic compounds (VOCs)

Amal H, Ding L, Liu BB, Tisch U, Xu ZQ, Shi DY, Zhao Y, Chen J, Sun RX, Liu H, Ye SL, Tang ZY, Haick H

International Journal of Nanomedicine 2012, 7:4135-4146

Published Date: 30 July 2012

Ultrasound sonication with microbubbles disrupts blood vessels and enhances tumor treatments of anticancer nanodrug

Lin CY, Tseng HC, Shiu HR, Wu MF, Chou CY, Lin WL

International Journal of Nanomedicine 2012, 7:2143-2152

Published Date: 24 April 2012