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Biosynthesis characterization of silver nanoparticles using Cassia roxburghii DC. aqueous extract, and coated on cotton cloth for effective antibacterial activity

Authors Balashanmugam P, Kalaichelvan PT

Received 12 January 2015

Accepted for publication 15 February 2015

Published 1 October 2015 Volume 2015:10(Supplement 1 Challenges in biomaterials research) Pages 87—97

DOI https://doi.org/10.2147/IJN.S79984

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Editor who approved publication: Dr Thomas J Webster


Pannerselvam Balashanmugam, Pudupalayam Thangavelu Kalaichelvan

Centre for Advanced Studies in Botany, University of Madras, Chennai, India

Abstract: The present study reports the green synthesis of silver nanoparticles (AgNPs) from silver precursor using a plant biomaterial, Cassia roxburghii DC., aqueous extract. The AgNPs were synthesized from the shade-dried leaf extract and assessed for their stability; they elucidated characteristics under UV–visible spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, high-resolution transmission electron microscopy, and energy dispersive X-ray spectroscopy. The synthesized AgNPs exhibited a maximum absorption at 430 nm, and the X-ray diffraction patterns showed that they were crystal in nature. Fourier transform infrared spectroscopy analysis confirmed the conversion of Ag+ ions to AgNPs due to the reduction by capping material of plant extract. The HR-TEM analysis revealed that they are spherical ranging from 10 nm to 30 nm. The spot EDAX analysis showed the presence of silver atoms. In addition, AgNPs were evaluated for their antibacterial activity against six different pathogenic bacteria: three Gram-positive bacteria, Bacillus subtilis, Staphylococcus aureus, and Micrococcus luteus, and three Gram-negative bacteria, Pseudomonas aeruginosa, Escherichia coli, and Enterobacter aerogenes. They were highly sensitive to AgNPs, whereas less sensitive to AgNO3. Furthermore, the green synthesized AgNPs were immobilized on cotton fabrics and screened for antibacterial activity. The immobilized AgNPs on cotton cloth showed high antibacterial activity. Therefore, they could be a feasible alternative source in treating wounds or may help in replacing pharmaceutical band-aids.

Keywords: bioreduction, stability, immobilization, cotton cloth, minimum inhibitory concentration

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