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Biosynthesis, characterization, and anticancer effect of plant-mediated silver nanoparticles using Coptis chinensis

Authors Pei J, Fu B, Jiang L, Sun T

Received 20 September 2018

Accepted for publication 13 December 2018

Published 15 March 2019 Volume 2019:14 Pages 1969—1978

DOI https://doi.org/10.2147/IJN.S188235

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Cristina Weinberg

Peer reviewer comments 3

Editor who approved publication: Dr Linlin Sun


Junwen Pei, Binfan Fu, Lifeng Jiang, Taizhen Sun

Department of Integrated Traditional Chinese and Western Medicine, The Henan Cancer Hospital, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan 450008, China

Background: Tremendous growth in nanotechnology has opened up new frontiers in fundamental and applied aspects, including the synthesis of nanoscale matter and understanding/utilizing its exotic physicochemical and optoelectronic properties. Green-synthesis methods employing either biological microorganisms or plant extracts have emerged as a simple and alternative to chemical synthesis.
Methods: In our present study, we aimed to synthesize silver nanoparticles (AgNPs) in combination with an aqueous extract of Coptis chinensis (CC) using a suitable ecofriendly green-synthesis way.
Results: In our results, ultraviolet-visible spectroscopy revealed a near-absorbance peak at 450 nm, which confirmed the AgNP synthesis. The crystalline nature of the AgNPs was revealed with X-ray diffraction. Transmission electron-microscopy analysis showed spherically dispersed nanoparticles of 6–45 nm diameter. We analyzed the elementary mechanism across A549 lung carcinoma cells ahead of treatment with doses of CC-AgNPs (10 µg/mL and 25 µg/mL). The antiproliferative effect of CC-AgNPs revealed a significant decline in cell viability. Antibacterial assays with both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria exhibited a higher zone of inhibition against S. aureus.
Conclusion: Furthermore, CC-AgNPs regulated apoptosis using the intrinsic pathway to inhibit A549-cell proliferation. Proliferation migration and invasion were notably inhibited by CC-AgNPs, which promoted apoptosis in lung adenocarcinoma cells by regulating the apoptotic pathway.

Keywords: Coptis chinensis, silver nanoparticles, antimicrobial, lung cancer, apoptosis, invasion

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