Association study of IGF1 polymorphisms with susceptibility to high myopia in a Japanese population

Masao Yoshida,^1,* Akira Meguro,^2,* Atsushi Yoshino,² Naoko Nomura,² Eiichi Okada,³ Nobuhisa Mizuki<sup>2

1</sup>Department of Public Health, Kyorin University School of Medicine, Mitaka, Tokyo, Japan; ²Department of Ophthalmology and Visual Science, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan; ³Okada Eye Clinic, Yokohama, Kanagawa, Japan

*These authors contributed equally to this work

Purpose: Polymorphisms in the insulin-like growth factor 1 (IGF1) gene were previously associated with high or extreme myopia in Caucasian and Chinese populations. In the present study, we investigated whether IGF1 polymorphisms are associated with high myopia in a Japanese population.
Methods: A total of 446 Japanese patients with high myopia (≤–9.00 diopters) and 481 Japanese healthy controls (+1.50 diopters to –1.50 diopters) were recruited. We genotyped seven tagging single-nucleotide polymorphisms (SNPs) in IGF1 and assessed allelic and haplotypic diversity in cases and controls.
Results: There were no statistically significant differences in the allele frequencies of IGF1 SNPs and genotypes between cases and controls (P>0.05). However, the A allele of rs5742629 and the G allele of rs12423791 were associated with a moderately increased risk of high myopia (odds ratio [OR] =1.20 and OR =1.21, respectively) with borderline statistical significance (P=0.0502, corrected P (Pc) =0.21 and P=0.064, Pc=0.29, respectively). The haplotype consisting of the A allele of rs5742629 and the G allele of rs12423791 was marginally associated with the risk of high myopia (P=0.041; OR =1.21); this association was not significant after correction (Pc=0.19).
Conclusion: We found that the IGF1 SNPs are not significantly associated with high myopia in our Japanese population. Our results are in contrast to a previous study in which extreme myopia cases had significantly higher frequencies of the G allele of rs5742629 and the C allele of rs12423791 than controls. Therefore, the IGF1 SNPs may not be important factors for susceptibility to high myopia in all populations. Further genetic studies are needed to elucidate the possible contributions of the IGF1 region to the development of high myopia.

Keywords: high myopia, IGF1, association study, polymorphism