Back to Browse Journals » International Journal of Nanomedicine » Volume 2 » Issue 4

In vitro imaging of embryonic stem cells using multiphoton luminescence of gold nanoparticles

Authors D Nagesha, GS Laevsky, P Lampton, R Banyal, C Warner

Published 15 January 2008 Volume 2007:2(4) Pages 813—819

D Nagesha1, GS Laevsky2, P Lampton3, R Banyal1, C Warner3, C DiMarzio2, S Sridhar1

1Electronic Materials Research Institute and Department of Physics, 2Electrical and Computer Engineering Department, 3Department of Biology, Northeastern University, Boston, MA, USA

Abstract: Recent advances in nonlinear optical techniques and materials such as quantum wells, nanowires and noble-metal nanoparticles have led to advances in cellular imaging wherein various nanoparticles have been shown to improve both in vitro and in vivo visualization. In this paper, we demonstrate in vitro imaging using multi-photon photoluminescence of gold nanoparticles from two different cell types – Dictyostelium discoideum and mouse embryonic stem cells. By observing nanoparticles we show that embryonic stem cells maintained their ability to proliferate for several passages while grown in the presence of gold nanoparticles. The advantages of multi-photon luminescence using gold nanoparticles have important implications for use in stem cell proliferation experiments and in vitro experiments to monitor differentiation.

Keywords: gold nanoparticles, multiphton absorption induced luminescence, mouse embryonic stem cells, visualization

Download Article [PDF] 

Readers of this article also read:

Fecal carriage of CTX-M β-lactamase-producing Enterobacteriaceae in nursing homes in the Kinki region of Japan

Luvsansharav UO, Hirai I, Niki M, Nakata A, Yoshinaga A, Yamamoto A, Yamamoto M, Toyoshima H, Kawakami F, Matsuura N, Yamamoto Y

Infection and Drug Resistance 2013, 6:67-70

Published Date: 22 July 2013

Exosome nanovesicles displaying G protein-coupled receptors for drug discovery

Angeles Estelles, Jeff Sperinde, Thibaut Roulon, Barbara Aguilar, Caroline Bonner

International Journal of Nanomedicine 2007, 2:751-760

Published Date: 15 January 2008

Clinical applications of perfluorocarbon nanoparticles for molecular imaging and targeted therapeutics

Trung D Tran, Shelton D Caruthers, Michael Hughes, John N Marsh, Tillmann Cyrus, et al

International Journal of Nanomedicine 2007, 2:515-526

Published Date: 15 January 2008

On prediction of optical properties of two- and multiphase nanocomposites for nanomedicine

Kai-Erik Peiponen, Evgeny Gornov

International Journal of Nanomedicine 2007, 2:799-804

Published Date: 15 January 2008

Pooled results of two randomized clinical trials comparing the efficacy and safety of travoprost 0.004%/timolol 0.5% in fixed combination versus concomitant travoprost 0.004% and timolol 0.5%

Ronald L Gross, E Kenneth Sullivan, David T Wells, Sushanta Mallick, Theresa A Landry, et al

Clinical Ophthalmology 2007, 1:317-322

Published Date: 15 December 2007

Anterior segment dysgenesis in a child with factor VII deficiency

Francis Beby, Sandrine Meunier, Pierre Cochat, Olivier Roche, Florent Aptel, et al

Clinical Ophthalmology 2007, 1:335-337

Published Date: 15 December 2007

A review of Perindopril in the reduction of cardiovascular events

Duncan J Campbell

Vascular Health and Risk Management 2006, 2:117-124

Published Date: 15 June 2006

Dietary approaches that delay age-related diseases

Arthur V Everitt, Sarah N Hilmer, Jennie C Brand-Miller, Hamish A Jamieson, A Stewart Truswell, Anita P Sharma, Rebecca S Mason, Brian J Morris, David G Le Couteur

Clinical Interventions in Aging 2006, 1:11-31

Published Date: 15 March 2006

Selective adhesion and mineral deposition by osteoblasts on carbon nanofiber patterns

Dongwoo Khang, Michiko Sato, Rachel L Price, Alexander E Ribbe Thomas J Webster

International Journal of Nanomedicine 2006, 1:65-72

Published Date: 15 March 2006