Back to Browse Journals » International Journal of Nanomedicine » Volume 2 » Issue 4

In vitro imaging of embryonic stem cells using multiphoton luminescence of gold nanoparticles

Authors D Nagesha, GS Laevsky, P Lampton, R Banyal, C Warner

Published 15 January 2008 Volume 2007:2(4) Pages 813—819

D Nagesha1, GS Laevsky2, P Lampton3, R Banyal1, C Warner3, C DiMarzio2, S Sridhar1

1Electronic Materials Research Institute and Department of Physics, 2Electrical and Computer Engineering Department, 3Department of Biology, Northeastern University, Boston, MA, USA

Abstract: Recent advances in nonlinear optical techniques and materials such as quantum wells, nanowires and noble-metal nanoparticles have led to advances in cellular imaging wherein various nanoparticles have been shown to improve both in vitro and in vivo visualization. In this paper, we demonstrate in vitro imaging using multi-photon photoluminescence of gold nanoparticles from two different cell types – Dictyostelium discoideum and mouse embryonic stem cells. By observing nanoparticles we show that embryonic stem cells maintained their ability to proliferate for several passages while grown in the presence of gold nanoparticles. The advantages of multi-photon luminescence using gold nanoparticles have important implications for use in stem cell proliferation experiments and in vitro experiments to monitor differentiation.

Keywords: gold nanoparticles, multiphton absorption induced luminescence, mouse embryonic stem cells, visualization

Download Article [PDF] 

Readers of this article also read:

Impact of JAK2(V617F) mutation status on treatment response to anagrelide in essential thrombocythemia: an observational, hypothesis-generating study

Cascavilla N, De Stefano V, Pane F, Pancrazzi A, Iurlo A, Gobbi M, Palandri F, Specchia G, Liberati AM, D’Adda M, Gaidano G, Fjerza R, Achenbach H, Smith J, Wilde P, Vannucchi AM

Drug Design, Development and Therapy 2015, 9:2687-2694

Published Date: 18 May 2015

Impact of β-blocker selectivity on long-term outcomes in congestive heart failure patients with chronic obstructive pulmonary disease

Kubota Y, Asai K, Furuse E, Nakamura S, Murai K, Tsukada YT, Shimizu W

International Journal of Chronic Obstructive Pulmonary Disease 2015, 10:515-523

Published Date: 5 March 2015

Nanopharmaceuticals (part 2): products in the pipeline

Weissig V, Guzman-Villanueva D

International Journal of Nanomedicine 2015, 10:1245-1257

Published Date: 11 February 2015

Synthesis of silver nanoparticles with antibacterial activity using the lichen Parmotrema praesorediosum

Mie R, Samsudin MW, Din LB, Ahmad A, Ibrahim N, Adnan SNA

International Journal of Nanomedicine 2014, 9:121-127

Published Date: 19 December 2013

Nanoemulsion improves the oral bioavailability of baicalin in rats: in vitro and in vivo evaluation

Zhao L, Wei Y, Huang Y, He B, Zhou Y, Fu J

International Journal of Nanomedicine 2013, 8:3769-3779

Published Date: 2 October 2013

Bioreducible and acid-labile poly(amido amine)s for efficient gene delivery

Yu ZQ, Yan JJ, You YZ, Zhou QH

International Journal of Nanomedicine 2012, 7:5819-5832

Published Date: 23 November 2012

Downregulation of VEGF mRNA expression by triamcinolone acetonide acetate-loaded chitosan derivative nanoparticles in human retinal pigment epithelial cells

Zhou H, Yang L, Li H, Gong H, Cheng L, Zheng H, Zhang L, Lan Y

International Journal of Nanomedicine 2012, 7:4649-4660

Published Date: 22 August 2012

Synthesis, characterization, and in vivo efficacy evaluation of PGG–docetaxel conjugate for potential cancer chemotherapy

Yang D, Van S, Shu Y, Liu X, Ge Y, Jiang X, Jin Y, L Yu

International Journal of Nanomedicine 2012, 7:581-589

Published Date: 3 February 2012

Preventing postoperative abdominal adhesions in a rat model with PEG-PCL-PEG hydrogel

Yang B, Gong C, Zhao X, Zhou S, Li Z, Qi X, Zhong Q, Luo F, Qian Z

International Journal of Nanomedicine 2012, 7:547-557

Published Date: 2 February 2012