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Anticancer activities of self-assembled molecular bowls containing a phenanthrene-based donor and Ru(II) acceptors

Authors Kim I, Song YH, Singh N, Jeong YJ, Kwon JE, Kim H, Cho Y, Kang SC, Chi K, An SSA

Received 9 May 2015

Accepted for publication 20 June 2015

Published 25 August 2015 Volume 2015:10(Special Issue on diverse applications in Nano-Theranostics) Pages 143—153

DOI https://doi.org/10.2147/IJN.S88287

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Prof. Dr. Thomas J. Webster


Inhye Kim,1,* Young Ho Song,2,* Nem Singh,2 Yong Joon Jeong,3 Jung Eun Kwon,3 Hyunuk Kim,4 Young Mi Cho,3 Se Chan Kang,3 Ki-Whan Chi2

1Laboratory of Bio-Resources, Yongin-si, Gyeonggi-Do, 2Department of Chemistry, University of Ulsan, Ulsan, 3Department of Life Science, Gachon University, Seongnam, 4Energy Materials Lab, Korea Institute of Energy Research, Daejeon, Republic of Korea

*These authors contributed equally to this work

Abstract: Nano-sized multinuclear ruthenium complexes have rapidly emerged as promising therapeutic candidates with unique anticancer activities. Here, we describe the coordination-driven self-assembly and anticancer activities of a set of three organometallic tetranuclear Ru(II) molecular bowls. [2+2] Coordination-driven self-assembly of 3,6-bis(pyridin-3-ylethynyl)phenanthrene (bpep) (1) and one of the three dinuclear arene ruthenium clips, [(ƞ6-p-iPrC6H4Me)2Ru2-(OO\OO)][OTf]2 (OO\OO =2,5-dioxido-1,4-benzoquinonato, OTf = triflate) (2), 5,8-dioxido-1,4-naphthoquinonato (3), or 6,11-dioxido-5,12-naphthacenediona (4), resulted in three molecular bowls 5–7 of general formula [{(ƞ6-p-iPrC6H4Me)2Ru2-(OO\OO)}2(bpep)2][OTf]4. All molecular bowls were obtained as triflate salts in very good yields (>90%) and were fully characterized using multinuclear nuclear magnetic resonance (NMR), electrospray ionization–mass spectrometry (ESI-MS), and elemental analysis. The structure of the representative molecular bowl 5 was confirmed by single-crystal X-ray diffraction analysis. The anticancer activities of molecular bowls 5–7 were determined by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, autophagy, and Western blot analysis. Bowl 6 showed the strongest cytotoxicity in AGS human gastric carcinoma cells and was more cytotoxic than doxorubicin. In addition, autophagic activity and the ratio of apoptotic cell death increased in AGS cells by treatment with bowl 6. Bowl 6 also induced autophagosome formation via upregulation of p62 and promotion of the conversion of LC3-I to LC3-II. Moreover, bowl 6 promoted apoptotic cell death through downregulation of Akt/mTOR activation, followed by increased caspase-3 activity. These results suggest that bowl 6 induces gastric cancer cell death via modulation of autophagy and apoptosis. Bowl 6 is a potent anticancer agent and a potential treatment for human gastric cancer that merits further study.

Keywords: phenanthrene, ruthenium, coordination self-assembly, gastric cancer, Akt/mTOR

Introduction

Research on nano-sized Ru(II)-based self-assembled architectures as anticancer compounds has increased rapidly following successful assessments of Ru-based small molecules as treatments for metastatic solid tumors in clinical trials.16 Thus, a growing number of Ru(II)-based, self-assembled large two-dimensional and three-dimensional architectures have been developed which have shown promising results in human cancer cell lines as antitumor agents.714 Therrien and others exploited the self-assembly approach to design finite, nano-sized molecular architectures by combining organometallic half-sandwich acceptor clips and pyridyl-based donors.1518 Nanostructures based on half-sandwich arene–ruthenium complexes are promising candidates for cancer treatments, as they possess advantageous features such as low toxicity and a balance of hydrophilicity and hydrophobicity, a key issue for their transport in biological media.19 Phenanthrene-derived synthetic and natural products have demonstrated a variety of pharmacological effects, such as cytotoxicity, phytotoxicity, and anti-inflammatory, antimicrobial, antioxidant, antiplatelet aggregation, and antiallergic activities.2024 These important findings laid the foundation for the design of new nano-sized self-assembled architectures with phenanthrene-derived donors and arene-Ru–based acceptors, with the expectation of improved biological results in comparison with available chemotherapeutic agents. Studies on similar complexes suggest that phenanthrene-derived donors and arene-Ru–based acceptors will work together in a synergistic manner as complementary building blocks; the phenanthrene unit will interact with DNA, whereas the ruthenium center will bind to proteins.6 Anthracene,17 naphthelenediimide,11 carbazole,8 and many other dipyridyl donors have been used in the self-assembly of various molecular architectures9 that have shown promising anticancer activities; however, Ru-based architectures generated with phenanthrene derivatives have not been explored.

The three primary types of programmed cell death (PCD) are apoptosis (the predominant type of PCD, type I PCD), autophagy (type II PCD), and necroptosis (type III PCD), which can be distinguished according to cellular and nuclear morphology. Chemotherapeutic agents can produce anticancer effects by modulating autophagy and apoptosis. Apoptosis is related to the activation of caspases and a stereotypical sequence of mitochondrial alterations.25,26 In recent years, apoptosis induction has been prioritized as a target mechanism of action for cancer treatment agents in development. However, chemo-resistance in cancer cells inhibits the proapoptotic effects of chemotherapeutic agents. Therefore, induction of autophagic cell death is considered to be another potential target mechanism that could be exploited to suppress tumor growth and alleviate treatment resistance in apoptosis-defective tumor cells.27 Autophagy is an evolutionarily catabolic process that plays a vital role in cellular degradation and cell survival.27 Although autophagy is normally maintained at a low basal level, dysregulated autophagy is observed in cancer pathology, inflammatory diseases, metabolic diseases, and neurodegenerative diseases.2831 Recently, autophagy has been noted as a target for anticancer drugs that suppress tumorigenesis.32,33 Additionally, a number of studies have suggested that autophagy is closely connected with apoptosis, as highlighted by the finding that the induction of apoptosis is often related to increased autophagy.34,35 Therefore, accumulating studies indicate that autophagy-modulating agents may provide a new wave of therapeutic drugs and strategies for cancer treatment.

Herein, we present a set of three tetranuclear Ru(II) metalla-bowls 57 incorporating a new phenanthrene-derived donor 1 and dinuclear Ru(II) p-cymene acceptors 24. All three molecular bowls were evaluated for their cytotoxicity against human tumor cell lines. Bowl 6 was found to show remarkable selectivity for the AGS human gastric carcinoma cell line. Furthermore, we found that bowl 6 induced autophagy and apoptosis by inducing conversion of LC3-I to LC3-II and inhibiting the Akt/mTOR signaling pathway.

Materials and methods

All chemicals used in this work were purchased from commercial sources and used without further purification. All solvents were distilled by standard methods prior to use. The starting arene-ruthenium acceptor clips10,36 and 3,6-dibromophenanthrene37 were prepared as previously described. The 1H and 13C NMR spectra were recorded with a Bruker 300 MHz spectrometer. Mass spectra for the self-assembled architectures were recorded using electrospray ionization with a MassLynx operating system at the Korea Basic Science Institute (Seoul, Korea). Elemental analyses were performed at the Technical Support Centre, Pohang University of Science and Technology.

Crystallographic data collection and structure refinement

The diffraction data from single crystals mounted on a loop were collected at 100 K on an ADSC Quantum 210 CCD diffractometer with synchrotron radiation (λ =0.80000 Å) at Macromolecular Crystallography Beamline 2D, Pohang Accelerator Laboratory, Pohang, Korea. The raw data were processed and scaled using the HKL2,000 program. The structure was solved by direct methods, and the refinements were carried out with full-matrix least squares on F2 with appropriate software implemented in the SHELXTL program package. X-ray data for complex 5: C113H96F12N4O21Ru4S4, M=2,606.46, triclinic, P, a=16.457(3) Å, b=18.067(4) Å, c=22.099(4) Å, α=68.36(3)°, β=81.25(3)°, γ=64.86(3)°, V=5,527.2(19) Å3, Z=2, T=100 K, μ(synchrotron) =0.504 mm−1, ρcalcd =1.566 g cm−3, 26,273 reflections measured, R1 =0.0670 and wR2 =0.2255 for 26,273 reflections (I >2σ(I)), R1 =0.1163 and wR2 =0.2834 (all data), GoF =1.233, 1,498 parameters, and 195 restraints, CCDC:1062887. All of the non-hydrogen atoms were refined anisotropically. Hydrogen atoms were added to their geometrically ideal positions.

Synthesis of dipyridyl phenanthrene donor 1

To a round-bottom flask, 3,6-dibromophenanthrene (89.6 mg, 0.267 mmol), 3-ethynylpyridine (63.3 mg, 0.613 mmol), CuI (2.5 mg, 0.013 mmol), [PdCl2(PPh3)2] (11.2 mg, 0.016 mmol), and PPh3 (4.2 mg, 0.016 mmol) were added. THF (5 mL) and triethylamine (5 mL) were added to the flask and bubbled with N2 for 10 minutes. The resulting reaction mixture was refluxed under nitrogen for 2 days and cooled, after which the solvent was removed under vacuum. The resulting residue was purified by flash column chromatography (SiO2, ethyl acetate with 2% triethylamine) to afford 1 as a white solid. Yield: 48%. Mp: 221°C–223°C. Anal calcd for C28H16N2: C, 88.40; H, 4.24; N, 7.36. Found: C, 88.12; H, 4.22; N, 7.40. 1H NMR (300 MHz, CD3NO2/CD3OD) δ 9.03 (d, J=1.5 Hz, 1H), 8.82 (dd, J=1.8, 0.9 Hz, 1H), 8.56 (dd, J=4.8, 1.8 Hz, 1H), 8.07 (dd, J=7.9, 1.8 Hz, 1H), 8.01 (d, J=8.3 Hz, 1H), 7.88 (s, 1H), 7.82 (dd, J=8.3, 1.5 Hz, 1H), 7.51 (ddd, J=7.9, 4.8, 0.9 Hz, 1H). 13C NMR (75 MHz, CDCl3) δ 152.17, 148.53, 138.99, 132.33, 129.82, 129.69, 129.04, 127.83, 126.74, 123.45, 121.04, 120.75, 93.57, 86.91. HRMS for C28H16N2: calcd, 380.1313; observed, 380.1314.

General procedure for the self-assembly of molecular bowls 5–7

Phenanthrene donor 1 and an arene-Ru(II) acceptor 2, 3, or 4 were stirred in 1.5 mL nitromethane/methanol (1:1) at room temperature for 6 hours to obtain a clear solution, to which diethyl ether was added dropwise to precipitate the product, which was washed twice with diethyl ether via centrifugation. The resulting crystalline powders were dried to obtain pure samples of the molecular bowls.

Molecular bowl 5

Molecular bowl 5 was self-assembled by the general procedure described using phenanthrene donor 1 (1.14 mg, 0.003 mmol) and acceptor 2 (2.72 mg, 0.003 mmol). The dark-red colored crystalline powder was characterized as 5 (3.55 mg, yield: 92%). Mp: 233°C–235°C (dec.). Anal. calcd for C112H92F12N4O20Ru4S4: C, 52.25; H, 3.60; N, 2.18. Found: C, 52.39; H, 3.59; N, 2.17. 1H NMR (300 MHz, CD3NO2/CD3OD) δ 8.41 (s, 2H), 8.37 (s, 2H), 8.24 (d, J=7.1 Hz, 2H), 8.03 (d, J=5.7 Hz, 2H), 7.53–7.36 (m, 8H), 6.07 (d, J=6.3 Hz, 4H), 5.84 (d, J=6.3 Hz, 6H), 2.91 (d, J=6.9 Hz, 2H), 2.22 (s, 6H), 1.35 (d, J=6.9 Hz, 12H). 13C NMR (75 MHz, CD3NO2/CD3OD) δ 184.77, 155.68, 152.58, 142.90, 133.28, 130.20, 129.95, 129.68, 128.69, 127.05, 124.31, 124.03, 120.62, 119.80, 105.49, 102.82, 100.41, 97.07, 84.69, 84.65, 82.76, 32.54, 22.46, 18.29. ESI-MS for C109H92F3N4O11Ru4S: calcd, 709.42 [5-3OTf]+3; observed, 709.32.

Molecular bowl 6

Molecular bowl 6 was self-assembled by the general procedure described using phenanthrene donor 1 (1.14 mg, 0.003 mmol) and acceptor 3 (2.87 mg, 0.003 mmol). The greenish colored crystalline powder was characterized as 6. Isolated yield: 90%. Mp: 238°C–239°C (dec.). Anal. calcd for C120H96F12N4O20Ru4S4: C, 53.89; H, 3.62; N, 2.09. Found: C, 53.81; H, 3.63; N, 2.10. 1H NMR (300 MHz, CD3NO2/CD3OD) δ 8.50 (d, J=1.7 Hz, 2H), 8.38 (s, 2H), 8.20 (d, J=4.7 Hz, 2H), 8.16 (d, J=7.9 Hz, 2H), 7.51 (d, J=8.2 Hz, 2H), 7.44–7.32 (m, 6H), 7.26 (s, 4H), 5.84 (d, J=6.3 Hz, 4H), 5.59 (d, J=6.3 Hz, 4H), 2.89 (d, J=6.9 Hz, 2H), 2.14 (s, 6H), 1.34 (d, J=6.9 Hz, 12H). 13C NMR (75 MHz, CD3NO2/CD3OD) δ 171.91, 154.93, 151.80, 142.60, 138.66, 133.19, 130.32, 129.86, 129.72, 128.60, 127.03, 126.44, 123.83, 120.72, 119.81, 112.59, 104.81, 101.26, 96.42, 85.65, 85.00, 83.73, 31.94, 22.40, 17.43. ESI-MS for C117H96F3N4O11Ru4S: calcd, 742.77 [6-3OTf]+3; observed, 742.95.

Molecular bowl 7

Molecular bowl 7 was self-assembled by the general procedure described using phenanthrene donor 1 (1.14 mg, 0.003 mmol) and acceptor 4 (3.17 mg, 0.003 mmol). The dark-green colored crystalline powder was characterized as 7. Isolated yield: 94%. Mp: 240°C–241°C (dec.). Anal. calcd. for C136H104F12N4O20Ru4S4: C, 56.82; H, 3.65; N, 1.95. Found: C, 56.89; H, 3.64; N, 1.96. 1H NMR (300 MHz, CD3NO2/CD3OD) δ 8.74 (dd, J=5.8, 3.3 Hz, 4H), 8.60 (s, 2H), 8.21 (d, J=5.6 Hz, 2H), 8.16 (s, 2H), 8.06 (dd, J=5.8, 3.3 Hz, 4H), 7.98 (d, J=8.1 Hz, 2H), 7.44 (d, J=8.2 Hz, 2H), 7.34 (s, 2H), 7.19 (dd, J=8.1, 5.7 Hz, 2H), 7.11 (d, J=8.2 Hz, 2H), 6.04 (d, J=6.3 Hz, 4H), 5.75 (d, J=6.3 Hz, 4H), 3.05 (dt, J=13.9, 6.9 Hz, 2H), 2.26 (s, 6H), 1.39 (d, J=6.9 Hz, 12H). 13C NMR (75 MHz, CD3NO2/CD3OD) δ 170.33, 155.58, 151.69, 141.96, 134.87, 134.26, 133.06, 130.03, 129.82, 129.43, 128.54, 128.32, 126.83, 126.35, 123.52, 120.53, 119.80, 108.27, 104.42, 101.34, 96.15, 85.75, 84.80, 83.11, 32.07, 22.56, 17.92. ESI-MS for C133H104F3N4O11Ru4S: calcd 809.44 [7-3OTf]+3; observed, 809.49.

Cell culture and reagents

HCT-15 human colorectal cancer cells, SK-hep-1 human liver cancer cells, AGS human gastric cancer cells, and COS-7 monkey fibroblasts were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). The HCT-15, AGS, and COS-7 cells were cultured in Dulbecco’s Modified Eagle’s Medium. The SK-hep-1 cells were cultured in RPMI 1640 medium. The cells were supplemented with 5% fetal bovine serum and 1% penicillin/streptomycin and incubated at 37°C in a humidified atmosphere of 5% CO2. The human cell lines used in this experiment were handled in accordance with the guidelines for human cell line experiment. This study was approved by the Ethics Committee of Gachon University Institutional Animal Care and Use.

Cell viability assay

The cells (5×103 cells per well) were seeded into 96-well plates and incubated at 37°C overnight, followed by stimulation with each compound dissolved in dimethyl sulfoxide (DMSO) for the indicated periods of time. After incubation, the cells were cultured with 10 μL of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide solution for 4 hours at 37°C in a humidified atmosphere of 5% CO2. The formazan formed during the culture period was dissolved in 100 μL of DMSO. The absorbance of the resulting solution was measured at 550 nm using a microplate reader. The half-maximal inhibitory concentration (IC50) values for cell growth were calculated by fitting the plot of the logarithmic percentage of surviving cells against the logarithm of the drug concentration using a linear regression function.

Labeling of autophagic vacuoles with monodansylcadaverine

The autophagic activity of bowls 57 was determined by fluorescent monodansylcadaverine (MDC) staining. Cells were labeled with 0.05 mM MDC (Sigma-Aldrich, St Louis, MO, USA) in RPMI 1640 medium at 37°C for 10 minutes, after which they were washed three times with phosphate buffered saline (PBS) and immediately observed under a fluorescence microscope (Nikon Eclipse TE 300, Tokyo, Japan) equipped with a filter system (V-2A excitation filter, 380/420 nm; barrier filter, 450 nm).

Tali® image-based cytometric assay

Apoptosis was assessed using the Tali® Image-Based Cytometer (Thermo Fisher Scientific, Waltham, MA, USA) as described previously.38,39 Briefly, the AGS and COS-7 cells were treated with bowl 6 at concentrations from 0 μM to 20 μM for 24 hours at 37°C in an atmosphere of 5% CO2. The number of cells was measured using a Countess® Automated Cell Counter. The cells were harvested using TrypLE™ reagent and stained with the Tali® Apoptosis Kit. For the estimation of apoptotic cell death, the cells were stained with the annexin V–Alexa Fluor® 488 conjugate. Propidium iodide (PI) was used to differentiate the cells that were necrotic (annexin V positive/PI positive or annexin V negative/PI positive) from those that were apoptotic (annexin V positive/PI negative). The percentages of the population reported as viable, apoptosis, and necrosis by the Tali® cytometer were comparable with data obtained when the same samples were run independently on a flow cytometer.

Western blot analysis

Cultured cells were washed with cold PBS and lysed with RIPA lysis buffer (Santa Cruz Biotechnology, Inc., Dallas, TX, USA) to allow protein extraction. The protein concentration was determined using a commercial protein assay kit (Bio-Rad Laboratories Inc., Hercules, CA, USA). Bovine serum albumin was used for the standard curve. Equal amounts of each total protein lysate (20 μg) were resolved by 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred onto Immobilon®-P polyvinylidene difluoride membranes (Amersham, Arlington Heights, IL, USA), which were incubated overnight with primary antibodies. The membranes were subsequently probed with horseradish peroxidase–conjugated secondary antibodies and visualized using an enhanced chemiluminescence kit (Amersham).

Caspase-3 activity analysis

AGS cells were seeded into 96-well clear-bottom plates. Caspase-3 activity was measured using the Caspase-Glo_3 Assay kit (CG3 kit; Promega Corporation, Fitchburg, WI, USA) according to the manufacturer’s protocol. The CG3 Assay is based on the cleavage of the specific sequence of a luminogenic substrate by caspase-3, which results in a luminescent signal. Fluorescence was measured at an excitation wavelength of 485 nm and an emission wavelength of 527 nm using a Mikrowin 2,000 Plate Reader (Tecan, Männedorf, Switzerland).

Statistical analysis

Data are presented as mean ± standard error of mean Statistical analysis was determined by one-way analysis of variance using Sigma STAT (Jandel Software, San Raphael, CA, USA). Differences between individual groups were assessed using Dunnett’s test. Statistical significance was considered as P<0.05 and P<0.01.

Results and discussion

Synthesis and characterization of molecular bowls 5–7

Phenanthrene-derived donor 1 was prepared by Sonagashira coupling of 3,6-dibromophenathrene and fully characterized by multinuclear NMR and high-resolution mass spectroscopy (Figure 1).

Figure 1 Synthesis of phenanthrene donor 1.

The pure dipyridyl donor 1 was mixed separately with dinuclear Ru(II) p-cymene acceptors 24 and solvent CH3NO2/CH3OH (1/1) was added to each resulting mixture. The reaction mixtures were stirred at room temperature for 6 hours to obtain clear solutions. Precipitation and filtration via the addition of diethyl ether yielded 57 as analytically pure crystalline powders (Figure 2). The new molecular bowls 57 were fully characterized by 1H and 13C NMR, ESI-MS, and elemental analysis. The solid-state structure of complex 5 was determined by single-crystal X-ray analysis.

Figure 2 Self-assembly of molecular bowls 57.
Notes: Molecular bowl 5 was self-assembled using 1 and 2. Molecular bowl 6 was self-assembled using 1 and 3. Molecular bowl 7 was self-assembled using 1 and 4.

The 1H NMR spectra in CD3OD/CD3NO2 (1/1) clearly indicated the formation of molecular bowls 57 with typical resonance shifts relative to those of the free donor and acceptors due to metal–ligand binding.9 The α-pyridyl protons of donor 1 shifted upfield by 0.4–0.6 ppm on complexation with acceptors 24. The 1H NMR peaks associated with the phenanthrene unit also significantly shifted upfield. ESI-MS analysis confirmed the formation of molecular bowls 57. The isotopic distribution peaks for molecular bowls 5, 6, and 7 observed at m/z 709.32, 742.96, and 809.49, respectively, corresponded to [M−3OTf]3+ and were in good agreement with the theoretical isotopic distribution patterns for these compounds (Figure 3)

Figure 3 Electrospray ionization–mass spectrometry spectra of molecular bowls (A) [5-3OTf]3+, (B) [6-3OTf]3+, and (C) [7-3OTf]3+.

The molecular structure of bowl 5 was unambiguously confirmed by single-crystal X-ray analysis (Figure 4). A suitable single crystal of 5 for X-ray crystallography using synchrotron radiation was obtained by slow vapor diffusion of diethyl ether into a concentrated CH3NO2/CH3OH (1:1) solution containing 5. X-ray analysis revealed that the two phenanthrene units were closely stacked with strong π–π interactions at the interplanar distance of ~3.5 Å. The strong π–π interactions between phenanthrene units forced the molecule into a bowl-like shape. The maximum interatomic distance of the structure was found to be 1.975 nm between C107 and C106. The average Ru-N pyridine bond distances were 2.11 Å, which was comparable to that of other tetranuclear architectures.9 Two views of the structure of 5 are shown in Figure 4.

Figure 4 Two views of the X-ray crystal structure of bowl 5.
Note: The hydrogen atoms and counter anions are omitted for clarity.

Cytotoxicity of organometallic multinuclear bowls 57 against human cancer cell lines

We first assessed the cytotoxic potential of each organometallic multinuclear arene–Ru(II) complex by determining their IC50 values in three types of human cancer cells. For these experiments, the effects of the compounds were assessed in HCT-15, SK-hep-1, and AGS cells, which were derived separately from colon cancer, hepatic cancer, and gastric cancer cells. Among the tested compounds, donor 1 and bowls 57 showed the strongest anticancer potential in all tested cell types. Moreover, the IC50 of bowl 6 in AGS cells was 3.2-fold lower than that of doxorubicin. Cisplatin showed an IC50 >100 μM in AGS cells, indicating that, in comparison with the other tested cell types, gastric cancer cells showed decreased sensitivity to cisplatin (Table 1). Based on these results, we selected the AGS human gastric carcinoma cell line for further analysis of the promising anticancer properties of bowls 57. In order to observe the effects of longer treatments, AGS cells were treated with bowl 6 for 24 hours, 48 hours, and 72 hours (Table 2). We found that treatment of bowl 6 and cisplatin for 48 hours and 72 hours maintained the anticancer effects, whereas doxorubicin tended to lose its cytotoxic effects on cancer cells.

Table 1 IC50 values of donor 1 and bowls 57 against HCT-15, SK-hep-1, and AGS human cancer cell lines
Note: Data are presented as mean ± standard error from at least three independent experiments, as obtained by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay using exposure times of 24 hours.
Abbreviation: IC50, the drug concentration necessary for 50% inhibition of cell viability.

Table 2 IC50 values of bowl 6 against the AGS human gastric cancer cell lines
Note: Data are presented as mean ± standard error from at least three independent experiments, as obtained by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay using exposure times of 24 hours, 48 hours, and 72 hours.
Abbreviation: IC50, the drug concentration necessary for 50% inhibition of cell viability.

Autophagy and apoptosis in bowl 6–treated AGS cells

To investigate whether the cytotoxic effects of bowl 6 in AGS cells were mediated by autophagy or apoptotic cell death, we estimated relative autophagic activity. Green fluorescent protein–transduced cells were stained using the Tali® Viability Kit, while Dead Cell Red was used to stain AGS human gastric cancer cells and COS-7 normal monkey kidney fibroblast cells. Cells were treated with 20 μM Bowl 6 for 24 hours, and DAPI or MDC staining was observed under fluorescence microscope. The representative pictures showed that treatment of 20 μM Bowl 6 increased MDC-stained area compared to control cells, indicating that Bowl 6 stimulated the autophagic vacuoles (Figure 5A). As shown Figure 5B, bowl 6 at concentrations of 5 μM, 10 μM, and 20 μM significantly increased the autophagic activity of AGS cells by 27.1%, 48.6%, and 68.4%, respectively, in comparison with the untreated cells. In normal COS-7 cells, bowl 6 at concentrations of 5 μM, 10 μM, and 20 μM significantly increased autophagic activity by 12.5%, 18.9%, and 23.3%, respectively, in comparison with the untreated cells. At a concentration of 20 μM, bowl 6 significantly increased the autophagic activity of AGS cells by 1.4-fold in comparison with normal cells. As shown Figure 5C, bowl 6 markedly induced concentration-dependent apoptotic cell death in AGS cells, while normal COS-7 cells were less susceptible to bowl 6 and had a higher ratio of live cells than the AGS cells following treatment with the same concentration of bowl 6. These results suggest that bowl 6 is selectively cytotoxic to cancer cells via induction of autophagic activity and apoptotic cell death, while producing no significant changes in autophagy or apoptosis in normal fibroblasts.

Figure 5 Effects of bowl 6 on programmed cell death.
Notes: (A) The representative pictures of MDC, DAPI-stained cells with merged pictures in AGS cells. Cells were treated with 20 μM bowl 6 for 24 hours and DAPI or MDC staining was observed under fluorescence microscope. (B) Relative autophagic activity following exposure to bowl 6 in the AGS and COS-7 cell lines. The cells were treated with the samples for 24 hours. (C) Analysis of apoptotic cell death in the AGS and COS-7 cell lines. Live cells and apoptotic cells were stained red and green. Apoptosis was assessed using a Tali® Image-Based Cytometer (Thermo Fisher Scientific). The results are mean ± standard error of mean (SEM) of triplicates from a representative experiment. *P<0.05, **P<0.01 compared to 0.
Abbreviation: MDC, monodansylcadaverine.

Effects of autophagy and apoptosis induced by bowl 6 in AGS cells

We examined the cellular mechanisms through which bowl 6 activated autophagy in AGS cells by measuring the expression of p62, a selective target of autophagy, and the intracellular distribution of LC3 protein, a reliable autophagosome marker, using Western blotting. p62 protein is a commonly used marker for the estimation of autophagic flux that binds to LC3 and is degraded during autophagy, leading to accumulation of p62 when autophagy is inhibited.12 LC3 plays a key role in the formation of autophagosomes and has been identified in two forms, a cytosolic form designated LC3-I and a membrane-bound form designated LC3-II.41 Increased conversion of LC3, in particular of LC3-I to LC3-II, is observed during autophagy. Bowl 6 significantly upregulated the expression of p62 protein and enhanced LC3-I to LC3-II conversion in the AGS cells in comparison with the untreated cells (Figure 6A).

Figure 6 Regulation of programmed cell death by bowl 6.
Notes: (A) LC3 and p62 protein levels indicated that bowl 6 induced an autophagic response. AGS and COS-7 cells were treated with bowl 6 at a concentration of 0–1 μM for 24 hours for the autophagic inducible protein assay. (B) Apoptotic and autophagic effects of bowl 6 on Akt and upstream expression in the AGS and COS-7 cell lines. Cells were treated with bowl 6 at a concentration of 0–20 μM for 24 hours for the Akt/mTOR Western blot analysis. Bar graphs of p62, LC3 and mTOR protein densitometry normalized by β-actin concentrations, and p-AKT was normalized by AKT. The results are mean ± standard error of mean (SEM) of triplicates from a representative experiment. *P<0.05, **P<0.01 compared to 0.
Abbreviations: LC3, light chain 3; mTOR, mammalian target of rapamycin.

To further elucidate the mechanisms underlying bowl 6–induced apoptosis in AGS cells, we assessed phosphorylation of Akt and mTOR proteins by Western blotting. In human cancers, activation of the Akt/mTOR pathway via phosphorylation leads to cancer cell proliferation and metastasis, while stimulating autophagy. Because inhibition of the Akt/mTOR pathway is associated with inhibition of autophagy in cancer cells, potential anticancer agents can maintain tumor survival or induce tumor growth.12,4143 Expression of p-Akt in the bowl 6–treated AGS cells significantly decreased in comparison with that in the untreated cells, which was in accordance with the downregulation of mTOR in comparison with the untreated cells (Figure 6B). To assess whether bowl 6 induced caspase-mediated apoptosis, caspase-3 activity was measured using a substrate cleavage–based assay. As shown Figure 7, bowl 6 significantly increased caspase-3 activity in a dose-dependent manner in comparison with that of the untreated cells. These results imply that bowl 6 induced cancer cell death by inhibiting the Akt/mTOR pathway, followed by activation of autophagy.

Figure 7 Caspase-3 activity in AGS cells following treatment with bowl 6.
Note: Caspase-3 activity was evaluated by a luminescence assay. The results are mean ± standard error of mean (SEM) of triplicates from a representative experiment. **P<0.01 compared to control.

Stability of bowl 6 in DMSO and cell culture medium

The stability of bowl 6 was examined in DMSO and culture medium (10% fetal bovine serum in Dulbecco’s Modified Eagle’s Medium), using conditions similar to those under which the drug is likely to be handled during the course of biological studies. Bowl 6 (10 μM) was preincubated in the culture medium at 37°C for various periods of time before it was added to the AGS cell culture. As a control experiment, bowl 6 was preincubated in DMSO at 37°C for the same periods of time. As shown in Figure 8, bowl 6 was stable in DMSO for 50 hours at a concentration of 10 μM. After preincubation in the culture medium for 24 hours, the inhibitory rate of bowl 6 decreased by 50%.

Figure 8 Loss of the growth-inhibitory activity of bowl 6 following preincubation in AGS cell culture medium.
Note: DMSO was used as a control.
Abbreviations: DMSO, dimethyl sulfoxide; h, hours.

Conclusion

In this study, we report the synthesis of three new ~2-nmr-sized tetranuclear molecular bowls by coordination-driven self-assembly of a phenanthrene-derived donor and Ru(II) p-cymene acceptors. Formation of metalla-rectangles was established by various spectroscopic techniques. The molecular structure of one of the molecular bowls was confirmed by single-crystal X-ray diffraction analysis. Bowl 6 produced cytotoxicity in AGS human gastric carcinoma cells by inducing autophagy via activation of the Akt/mTOR pathway. We propose that bowl 6 selectively induces autophagic and apoptotic PCD in cell lines derived from gastric cancer cells and may thus be a viable candidate drug for the treatment of gastric cancer. This study was the first set of in vitro observations regarding the antitumor effects of nano-sized Ru(II)-based, self-assembled architectures through induction of apoptosis and autophagy in human cancer cells. Future research should confirm the anticancer effects of self-assembled molecular bowls in vivo and study pharmaceutical kinetics of orally administrated self-assembled molecular bowls.

Acknowledgments

This study was supported by the Basic Science Research program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (NRF-20013R1A1A2006859) as well as by the Ministry of Education (NRF-2014R1A1A2007828) Priority Research Centers program (2009-0093818). X-ray diffraction experiments using synchrotron radiation were performed at the Pohang Accelerator Laboratory in Korea. HK also would like to thank the Research and Development Program of KIER (B5-2513).

Disclosure

The authors report no conflicts of interest in this work.


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