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Analysis of Bimatoprost-Induced changes on Rabbits eyelash Follicle: Clinical and Electron microscopic study

Authors Sayed KM, Mostafa EM, Mounir A, Abd Elhaliem NG, Alsmman AH

Received 30 August 2019

Accepted for publication 14 November 2019

Published 5 December 2019 Volume 2019:13 Pages 2421—2426

DOI https://doi.org/10.2147/OPTH.S229335

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Nicola Ludin

Peer reviewer comments 3

Editor who approved publication: Dr Scott Fraser


Khulood Muhammad Sayed,1 Engy Mohammed Mostafa,1 Amr Mounir,1 Nesreen G Abd Elhaliem,2 Alahmady Hamad Alsmman1

1The Department of Ophthalmology, Sohag Faculty of Medicine, Sohag University, Sohag, Egypt; 2The Department of Histology, Sohag Faculty of Medicine, Sohag University, Sohag, Egypt

Correspondence: Alahmady Hamad Alsmman
The Department of Ophthalmology, Sohag Faculty of Medicine, Sohag University, Sohag 82524, Egypt
Tel +201111102698
Email alahmady20@yahoo.com

Objective: To analyze ultrastructural changes of rabbits’ eyelash follicles treated with bimatoprost eye drops to increase our knowledge of how this drug works.
Methods: The study included 15 clinically healthy male rabbits. All rabbits were treated with bimatoprost 0.03% daily for 4 weeks with one drop of the topical eye drops applied to the conjunctival fornix of the right eyes; left eyes were used as controls. Eyelash lengths were measured before and after treatment. The eyelid of each animal was dissected for light and electron microscopic analysis.
Results: Both control and treated rabbit eyes matched regarding eyelash length before treatment (9.80±0.388mm vs 9.88±0.24mm) (P=0.108). There was a significant increase in eyelash length between control (9.75±0.33 mm) and treated rabbit eyes (11.60±0.46 mm) (P=0.369). Light and electron microscopy revealed, bimatoprost treated eyes had thick epidermis. The dermis contained two hairs growing out of the same hair follicle. Heavily keratinized Henle’s layer, the cortical cells (Cx) have prominent nucleolus and cytoplasm is studded with melanin granules.
Conclusion: Bimatoprost-induced eyelash changes were not restricted to increased eyelash length, thickness, and pigmentation but also showed increased number of eyelashes within the same hair follicle which were stronger and could resist pulling from the skin without any evidence of inflammatory cells within the specimens. These changes occurred as early as 1 month of treatment, giving rise to thoughts about the possibility of using bimatoprost eye drops as a prophylaxis against madarosis associated with chemotherapy if it is started 1 month before chemotherapy and continued afterwards, making eyelashes stronger and resistant to falling out.

Keywords: bimatoprost, rabbit, eyelash, hair follicle

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