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A novel plasmid carrying carbapenem-resistant gene blaKPC-2 in Pseudomonas aeruginosa

Authors Hu YY, Wang Q, Sun QL, Chen GX, Zhang R

Received 29 November 2018

Accepted for publication 26 February 2019

Published 14 May 2019 Volume 2019:12 Pages 1285—1288

DOI https://doi.org/10.2147/IDR.S196390

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Joachim Wink


Yan-Yan Hu, Qi Wang, Qiao-Ling Sun, Gong-Xiang Chen, Rong Zhang

2nd Affiliated Hospital of Zhejiang University, School of Medicine, Zhejiang University, Hangzhou, People’s Republic of China

Abstract: A carbapenem-resistant Pseudomonas aeruginosa strain PA1011 (ST463) was isolated from a patient in a surgical intensive care unit. PCR detection showed that PA1011 carried the blaKPC-2 gene. A plasmid was isolated and sequenced using the Illumina NextSeq 500 and PacBio RSII sequencing platforms. The plasmid was named pPA1011 and carried the carbapenem-resistant gene blaKPC-2. pPA1011 was a 62,793 bp in length with an average G+C content of 58.8%. It was identified as a novel plasmid and encoded a novel genetic environment of blaKPC-2 gene (ΔIS6-Tn3-ISKpn8-blaKPC-2-ISKpn6-IS26).

Keywords: plasmid, genetic environment, KPC, ST463, Pseudomonas aeruginosa

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