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A novel dissolution media for testing drug release from a nanostructured polysaccharide-based colon specific drug delivery system: an approach to alternative colon media

Authors Kotla N, Singh S, Maddiboyina B, Sunnapu O, Webster T

Received 26 September 2015

Accepted for publication 12 January 2016

Published 17 March 2016 Volume 2016:11 Pages 1089—1095

DOI https://doi.org/10.2147/IJN.S97177

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Govarthanan Muthusamy

Peer reviewer comments 4

Editor who approved publication: Dr Lei Yang

Niranjan G Kotla,1,2 Sima Singh,1,3 Balaji Maddiboyina,4 Omprakash Sunnapu,2 Thomas J Webster5,6

1School of Pharmaceutical Sciences, Lovely Professional University, Punjab, India; 2Technologies for the Advancement of Science, Institute for Stem Cell Biology and Regenerative Medicine, Bangalore, Karnataka, India; 3Department of Pharmaceutical Sciences, Birla Institute of Technology, Mesra, Ranchi, Jharkhand, India; 4Department of Pharmaceutics, Vishwabharathi College of Pharmaceutical Sciences, Guntur, Andhra Pradesh, India; 5Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 6Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia

Abstract: The aim of this study was to develop a novel microbially triggered and animal-sparing dissolution method for testing of nanorough polysaccharide-based micron granules for colonic drug delivery. In this method, probiotic cultures of bacteria present in the colonic region were prepared and added to the dissolution media and compared with the performance of conventional dissolution methodologies (such as media with rat cecal and human fecal media). In this study, the predominant species (such as Bacteroides, Bifidobacterium, Lactobacillus species, Eubacterium and Streptococcus) were cultured in 12% w/v skimmed milk powder and 5% w/v grade “A” honey. Approximately 1010–1011 colony forming units m/L of probiotic culture was added to the dissolution media to test the drug release of polysaccharide-based formulations. A USP dissolution apparatus I/II using a gradient pH dissolution method was used to evaluate drug release from formulations meant for colonic drug delivery. Drug release of guar gum/Eudragit FS30D coated 5-fluorouracil granules was assessed under gastric and small intestine conditions within a simulated colonic environment involving fermentation testing with the probiotic culture. The results with the probiotic system were comparable to those obtained from the rat cecal and human fecal-based fermentation model, thereby suggesting that a probiotic dissolution method can be successfully applied for drug release testing of any polysaccharide-based oral formulation meant for colonic delivery. As such, this study significantly adds to the nanostructured biomaterials’ community by elucidating an easier assay for colonic drug delivery.

Keywords:
probiotic media, colon specific drug delivery, dissolution methodologies, simulated colonic media, microbially triggered drug delivery

Introduction

Colon specific drug delivery systems (CDDS) afford the most advantages for many local treatments of colonic disorders/diseases such as colon cancer and inflammatory bowel disease (which is of two types, ie, Crohn’s disease and ulcerative colitis), and other local bacterial infections.1,2 The approaches which are commonly used for a drug to reach the colon include a prodrug design approach, a time-dependent system, a pH sensitive polymeric approach, and microflora-activated delivery systems.3,4 Among the aforementioned systems, the microflora-triggered delivery systems have been found to be the most promising because specific colonic enzymes degrade the polysaccharide coating for drug release.57 The main principle of action of microflora-activated systems is enzymatic degradation of polysaccharides (such as chitosan, inulin, guar gum, cellulose, xanthan gum, amylose, alginates, dextran, pectin, khaya, and albizia gum) present in the system by colonic bacteria.8,9

In vitro evaluation of these systems remains a challenge in dissolution studies inhibiting the further discovery of treatment regimes. Moreover, the anaerobic microflora that are highly specific to the colonic region are challenging to assimilate into USP dissolution methods. As a consequence, alternative dissolution approaches have been designed to better represent colonic conditions in vitro. A rationally developed dissolution method with a proper justification can be used to estimate drug release kinetics as well as the pH effect and hydrodynamic conditions of the gastrointestinal tract on drug release characteristics.

In order to fulfill these aforementioned requirements, it is essential that the dissolution method be discriminative, reproducible, biorelevant, and more importantly, scientifically justifiable. When a drug product application is sent to the regulatory agencies, the dissolution methodology and its use as a quality control tool are the essential parts of that application.10

In the last decade, a number of biorelevant media have been developed to simulate conditions encountered by formulations designed for colon targeting (Figure 1).

Figure 1 Current dissolution methods to test the drug release of polysaccharide-based CDDS.
Abbreviation: CDDS, colon-based drug delivery system.

The most commonly used dissolution testing methods for these delivery systems (which have few limitations11) involve the addition of enzymes,1117 rat cecal contents,1821 and human fecal slurries.2231

Under colonic conditions, enzymes are continuously replenished by the microbiota that secretes these enzymes. However, for the in vitro methods used, the concentration of the enzymes used in the media decreases. The second method using rat cecal content requires the sacrifice of a large number of animals and human fecal content usage is not pragmatic because it would not be practical to obtain fecal samples every time without significant batch-to-batch variations.10

In this paper, therefore, an approach was made to develop an animal-sparing dissolution media using probiotics to test the drug release of guar gum/Eudragit FS30D coated 5-fluorouracil granules (5-FU) (Figure 2). Dissolution media with probiotics were compared with existing rat cecal and human fecal media. Moreover, an important part of this work was to create micron granules with nanostructured roughness, since numerous studies have demonstrated that nanoroughness can decrease inflammation and immune system recognition.32,33 This is because nanoroughness can alter the surface energy of granules and control initial protein adsorption (such as decreased IgG adsorption) to inhibit inflammatory cell responses. Such events would allow the drug capsule to be more effective at delivering drugs to targeted sites.

Figure 2 Outline of drug release during in vitro dissolution testing of polysaccharide-based colon-targeted drug delivery system using (A) enzymes, (B) rat cecal contents, (C) human fecal contents, and (D) cultured probiotics media.

Materials and methods

Materials

5-FU was procured from Jackson Laboratories Pvt. Ltd., Punjab, India. Guar gum and Xanthan gum were obtained from Molychem Manufacturers Pvt. Ltd., Mumbai, India. Eudragit FS30D was acquired from Evonik Industries, Essen, Germany. Probiotics were purchased from Eris Life Sciences Pvt. Ltd., Gujarat, India. Grade “A” honey was obtained from Dabur India Ltd., New Delhi, India. Dry milk powder was purchased from Everyday Milk Products, New Delhi, India.

Preparation of 5-FU drug-loaded granule core

The granules were prepared by mixing 5-FU, guar gum, and xanthan gum at a ratio of 1:1:1 (15 g each), (Table 1), in a V-cone blender. Wet granulation of the prepared mass was carried out using purified water. The damp mass was passed through sieve number 10 initially; a larger size fraction was obtained by using sieve number 30 with a mesh size of 0.59 mm. The prepared granules were dried for 30 minutes in a hot air oven at 40°C–50°C.

Table 1 Composition of guar gum and Eudragit FS30D coated 5-fluorouracil granules

Coating of the prepared granules

The prepared granules were coated up to 20% (9 g guar gum in 900 mL water for 45 g granules bed) with guar gum in an accela cota coating pan (Thomas Engineering, Hoffman Estates, IL, USA). The process parameters were as follows: an inlet temperature of 40°C±2°C, product temperature of 37°C±2°C, and spray rate of 0.2 g/min. This gum layer acts as a triggering mechanism for the drug release in the colon by colonic microflora. Further, the granules were coated with 40% (ie, 18 g in 60 mL of Eudragit FS30D) Eudragit FS30D to retard the drug release in the stomach and small intestine.

Filling of coated granules in hard gelatin transparent capsules

The weight equivalent to 50 mg of coated granules of 5-FU were filled in hard gelatin capsules manually.

Determination of drug content

The weight equivalent to 50 mg of 5-FU was transferred to a volumetric flask containing about 50 mL of pH 6.8 phosphate buffer and mixed thoroughly. The remainder of the volume to 100 mL was made up with a pH 6.8 buffer. The solutions were filtered through 0.22 μm membrane filters. The drug content was measured using reverse-phase high performance liquid chromatography (RP-HPLC; LC-20AD; Shimadzu, Kyoto, Japan) at 265 nm.

Preparation of dissolution media

Preparation of fresh human fecal content media

Fresh human fecal slurries were generally used to study the fermentation of nonstarch polysaccharides.24 The solution was prepared by homogenizing fresh feces (5% w/v) obtained from healthy humans. Content was dissolved in 0.1 M sodium phosphate buffer (pH 6.8) under anaerobic conditions with carbon dioxide purging. These solutions were finally added to the dissolution vessel to mimic the colonic environment for drug release.

Preparation of rat cecal media

Thirty minutes prior to the fifth hour of the dissolution study, rats were anesthetized and killed. The abdomens were opened, ceca were traced, ligated at both the ends, dissected, and contents were pooled and added to the pH 6.8 phosphate buffer continuously bubbled with carbon dioxide. These solutions were finally added to the dissolution media to give a final cecal dilution of 4% w/v.31

Preparation of probiotic culture media

In order to conduct dissolution testing in the probiotic culture media, the probiotic culture needs to be activated and cultured in a suitable media. A composition of probiotics (total 5 billion; Bifidobacterium breve, Bifidobacterium longum, Bifidobacterium infantis, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus casei, Lactobacillus rhamnosus, Streptococcus thermophilus, and Saccharomyces boulardi) and prebiotics (Fructooligosaccharide-100 mg) were used for developing the probiotic dissolution media.

A 5% w/v solution of tempered Grade “A” honey was added to 12% w/v reconstituted nonfat dry milk (NDM) in a 100 mL volumetric flask, and the remaining volume to 100 mL was made up with sterile water. The sample was pasteurized at 70°C for 30 minutes and cooled to 37°C. A single capsule of probiotic powder was slurried in sterile water and was inoculated into the pasteurized milk media and incubated at 37°C for 24 hours under anaerobic conditions.34 The cultured media was counted for total bacteria, which was found to be 9.8×1010 colony forming units (CFU)/mL (1011–1012 CFU/mL normal microbiota count in colon). The prepared probiotic media was added to the dissolution chamber.

Dissolution studies

In vitro drug release of 5-FU granules using human fecal slurries

The drug release experiments were carried out in six 250 mL beakers which were immersed into the dissolution vessels (DS 8000; LAB INDIA, Mumbai, India).27 Six 5-FU capsules were placed in a solution containing 150 mL of pH 1.2 HCl buffer at 37°C±0.5°C for 2 hours at 100 rpm. After 4 hours, the pH was adjusted to 6.8–7.5 using 50 mL of phosphate buffer and sodium hydroxide.

At the end of the fourth hour, the media was degassed using CO2 to remove undissolved oxygen and anaerobic conditions were maintained in the media for 15 minutes. A 5% w/v solution of freshly prepared fecal content was added to the dissolution media, and the study was continued for up to 24 hours with continuous purging of CO2 throughout the study.

About 1.0 mL of each sample was withdrawn at regular time intervals and was replaced by the fresh media which was maintained under anaerobic conditions. The volume of the sample was adjusted to 10 mL and solutions were filtered by using 0.22 μm membrane filters. Twenty μL of this solution was injected into an HPLC (LC-20AD; Shimadzu). All the studies were repeated six times and the mean data were recorded. The stationary phase used was a Kinetex 5U C-18 Column (250×4.6 mm2 ID: particle size 5 μm), with a mobile phase [Isocratic mode] of 40 mM monobasic potassium phosphate buffer adjusted to pH 7.0. The flow rate was kept at 0.5 mL/min and the retention time was found to be 5.5 minutes at 265 nm. The experimental protocol was approved by the institutional animal ethical committee of Lovely Professional University, Punjab (letter number- 954/ac/06/CPCSEA/12/8).

In vitro drug release of 5-FU granules using rat cecal media

The drug release experiments were carried out in six 250 mL beakers which were immersed into the dissolution vessels (DS 8000; LAB INDIA). Six 5-FU capsules were placed in a solution containing 150 mL of pH 1.2 HCl buffer at 37°C±0.5°C for 2 hours at 100 rpm. After 4 hours, the pH was adjusted to 6.8 using 50 mL of phosphate buffer and sodium hydroxide. A 4% w/v solution of rat cecal content was added to the dissolution media and the study was continued for up to 24 hours with continuous purging of CO2. About 1.0 mL of each sample was withdrawn at regular time intervals and replaced by fresh media which was maintained under anaerobic conditions. The volume of the sample was adjusted to 10 mL and solutions were filtered using 0.22 μm membrane filters and analyzed using RP-HPLC.

In vitro drug release of 5-FU granules using probiotic culture

The drug release experiments were carried out in six 250 mL beakers which were immersed into the dissolution vessels (DS 8000; LAB INDIA). Six 5-FU capsules were placed in a solution containing 150 mL of pH 1.2 HCl buffer at 37°C±0.5°C for 2 hours at 100 rpm. Afterwards, the pH of the dissolution media was adjusted to 6.8–7.5 with 50 mL of pH 6.8 buffer and sodium hydroxide, and the study continued for another 1 hour. At the end of the third hour, the media was degassed using carbon dioxide gas to remove the undissolved oxygen and to maintain anaerobic conditions inside the media for 10 minutes. Then, the entire volume of the prepared probiotic culture (9.8×1010 CFU/mL) was added to the dissolution media and the study was continued up to 24 hours with continuous purging of CO2 throughout the study. About 1.0 mL of each sample was withdrawn at regular time intervals and replaced by the fresh media. The volume of the sample was adjusted to 10 mL and solutions were filtered by using 0.22 μm membrane filters and analyzed using RP-HPLC. The studies on the aforementioned 5-FU multiparticulate granules were also carried out in the same manner but without adding the probiotic culture, rat cecal content, and/or human fecal contents (ie, pH buffer media).

Results and discussion

Drug content in 5-FU granules and characterization

Results showed that the present granules were approximately 500–600 μm in diameter and showed nanoscale roughness (Figure 3). Again, implementing nanoscale roughness on the granules was important because numerous studies have indicated that nanoscale roughness can decrease inflammation and immune system recognition allowing the granules to reach their destination and release drugs.32,33 Of course, more testing is necessary to confirm this. Moreover, the drug content of the guar gum and Eudragit FS30D coated granules for 5-FU was found to be 96.5%.

Figure 3 SEM image of the granules of interest to the present study.
Note: Magnification, 100 microns.
Abbreviation: SEM, scanning electron microscopy.

Drug release comparison in different media

The in vitro release profile of guar gum and Eudragit FS30D coated 5-FU granules containing capsules in different dissolution media is shown in Figure 4. The study revealed that in all cases, the 5-FU release was less than 10% in upper gastric tract and had a tendency to accelerate in the presence of the probiotic media when compared to human fecal media as well as rat cecal media.

Figure 4 Dissolution profile of 5-FU granules in buffer, fecal, rat cecal, and probiotic media.
Note: Values represents mean of three determinations and bars represent ± SEM.
Abbreviations: SEM, standard error of the mean; 5-FU, 5-fluorouracil.

After 24 hours, the capsules subjected to media containing probiotic cultures showed a release rate of 91.61% as compared to media containing fecal slurries (67.88%) and rat cecal content (86.81%). The obtained results showed superior dissolution potential of the developed probiotic media over human fecal slurries and rat cecal content. Bifidobacterium, Lactobacillus species and Bacteroids were the most predominant bacteria in the media which degraded the guar gum and xanthan gum coating, triggering effective drug release in both the probiotic and rat cecal media compared to human fecal media. The results with the probiotic system were comparable to those obtained from rat cecal- and human fecal-based fermentation model, thereby suggesting that the probiotic dissolution method can be successfully applied.

Microbially triggering CDDS are based on the manipulation of the specific enzymatic degradation of the colon microflora (Bifidobacterium, Bacteroids, Lactobacillus, and enterobacteria). These colonic bacterial milieu are predominately anaerobic in nature and secrete enzymes that are capable of metabolizing various polysaccharides that escape digestion in the upper gastric tract. Colon microflora have the capability of fermenting innumerable types of substrates that have been left undigested in the small intestine, eg, di- and trisaccharides, polysaccharides, etc. For this fermentation, the microflora produce various enzymes like glucoronidase, xylosidase, arabinosidase, galactosidase, and azo reductazes. Because of the presence of the biodegradable enzymes only in the colon, the use of biodegradable polymers for colon-specific drug delivery seems to be more site-specific compared to pH- and time-dependent approaches.

Conclusion

Pharmaceutical approaches commonly used in CDDS include triggering by changes in pH or enzymatic conditions or activation by microbial flora of the gastrointestinal tract. An efficacious colon drug-delivery system needs an in-built mechanism in the delivery system to only respond to the physiological surroundings specific to the colon. Due to the lack of similarities in the upper and lower gastrointestinal physiological conditions, strategies which are generally used to achieve colonic drug delivery, comprise a timed releasing system, coating with pH sensitive polymer layers, prodrug methodology, and colonic microflora-activated delivery systems. Among all the systems, the microflora activated delivery systems have been found to be the most effective scaffold systems since the abrupt escalation of the bacteria population and associated enzymatic activities in the ascending colon signifies a noncontinuous event independent of gastrointestinal transit time and pH conditions. The foremost principle in microflora-activated systems is a series of polysaccharides which evade enzymatic degradation in the small intestine and that are predominantly metabolized by colon bacteria to release the therapeutic agents.

To date, in vitro characterization specific to in vitro dissolution of these systems remains a challenge in drug release studies. Moreover, the resident microbiota that are highly specific to the colon are difficult to incorporate into the USP dissolution methodologies. As a consequence, alternative dissolution approaches have been designed to better represent the colonic conditions. A rationally developed dissolution method with a proper justification can be used to estimate drug release kinetics as well as the impact of pH and hydrodynamic conditions of the gastrointestinal tract on drug release characteristics.

This study was aimed at developing an animal-sparing, cost-effective dissolution media to test the drug release of polysaccharide-based formulations meant for the colon, by using probiotics. Probiotic culture was used in dissolution media to mimic the colonic microflora conditions. This approach shows that the dissolution media used for evaluating the drug release of pharmaceuticals meant for colon-specific drug delivery is possible. The dissolution profiles in the current test media (probiotic media) were found to be very similar to those acquired with the rat cecal content and fecal slurries media. More specifically, the current technique shows the development of animal sparing dissolution media for testing the drug release of microgranules of nanorough polysaccharide-based formulations used for colon-specific drug delivery. Of course, the current system needs to be validated and tested with various other drug-polysaccharide-based delivery systems before widespread implementation should occur.

Acknowledgments

The financial assistance and facilities provided by the Dean and Chancellor of Lovely Professional University, Punjab, India, are gratefully acknowledged.

Disclosure

NGK declares that this work was from his MS dissertation work, supported by the Lovely Faculty of Applied Medical Sciences. The authors report no conflicts of interest in this work.


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Balasundaram G, Storey DM, Webster TJ

International Journal of Nanomedicine 2014, 9:1845-1853

Published Date: 15 April 2014

Colloidal graphite/graphene nanostructures using collagen showing enhanced thermal conductivity

Bhattacharya S, Dhar P, Das SK, Ganguly R, Webster TJ, Nayar S

International Journal of Nanomedicine 2014, 9:1287-1298

Published Date: 10 March 2014

Novel kojic acid-polymer-based magnetic nanocomposites for medical applications

Hussein-Al-Ali SH, El Zowalaty ME, Hussein MZ, Ismail M, Dorniani D, Webster TJ

International Journal of Nanomedicine 2014, 9:351-362

Published Date: 7 January 2014

Greater fibroblast proliferation on an ultrasonicated ZnO/PVC nanocomposite material

Maschhoff PM, Geilich BM, Webster TJ

International Journal of Nanomedicine 2014, 9:257-263

Published Date: 28 December 2013

Nanostructured polyurethane-poly-lactic- co-glycolic acid scaffolds increase bladder tissue regeneration: an in vivo study

Yao C, Hedrick M, Pareek G, Renzulli J, Haleblian G, Webster TJ

International Journal of Nanomedicine 2013, 8:3285-3296

Published Date: 28 August 2013

Nanostructured magnesium has fewer detrimental effects on osteoblast function

Weng L, Webster TJ

International Journal of Nanomedicine 2013, 8:1773-1781

Published Date: 6 May 2013

Nano-BaSO4: a novel antimicrobial additive to pellethane

Aninwene II GE, Stout D, Yang Z, Webster TJ

International Journal of Nanomedicine 2013, 8:1197-1205

Published Date: 22 March 2013

Reduced adhesion of Staphylococcus aureus to ZnO/PVC nanocomposites

Geilich BM, Webster TJ

International Journal of Nanomedicine 2013, 8:1177-1184

Published Date: 21 March 2013

Decreased cervical cancer cell adhesion on nanotubular titanium for the treatment of cervical cancer

Crear J, Kummer KM, Webster TJ

International Journal of Nanomedicine 2013, 8:995-1001

Published Date: 7 March 2013

Comparison study of ferrofluid and powder iron oxide nanoparticle permeability across the blood–brain barrier

Hoff D, Sheikh L, Bhattacharya S, Nayar S, Webster TJ

International Journal of Nanomedicine 2013, 8:703-710

Published Date: 13 February 2013

Increased healthy osteoblast to osteosarcoma density ratios on specific PLGA nanopatterns

Wang Y, Zhang L, Sun L, Webster TJ

International Journal of Nanomedicine 2013, 8:159-166

Published Date: 7 January 2013

Mechanisms of greater cardiomyocyte functions on conductive nanoengineered composites for cardiovascular applications

Stout DA, Yoo J, Noemi Santiago-Miranda A, Webster TJ

International Journal of Nanomedicine 2012, 7:5653-5669

Published Date: 13 November 2012

Cytotoxicity of selenium nanoparticles in rat dermal fibroblasts

Ramos JF, Webster TJ

International Journal of Nanomedicine 2012, 7:3907-3914

Published Date: 23 July 2012

Decreased Staphylococcus aureus biofilm formation on nanomodified endotracheal tubes: a dynamic airway model

Machado MC, Tarquinio KM, Webster TJ

International Journal of Nanomedicine 2012, 7:3741-3750

Published Date: 19 July 2012

Carbon nanotubes impregnated with subventricular zone neural progenitor cells promotes recovery from stroke

Moon SU, Kim J, Bokara KK, Kim JY, Khang D, Webster TJ, Lee JE

International Journal of Nanomedicine 2012, 7:2751-2765

Published Date: 1 June 2012

Erratum

Aninwene G II , Yao C, Webster TJ

International Journal of Nanomedicine 2012, 7:1573-1574

Published Date: 22 March 2012

Fructose-enhanced reduction of bacterial growth on nanorough surfaces

Durmus NG, Taylor EN, Inci F, Kummer KM, Tarquinio KM, Webster TJ

International Journal of Nanomedicine 2012, 7:537-545

Published Date: 1 February 2012

Reduced adhesion of macrophages on anodized titanium with select nanotube surface features

Rajyalakshmi A, Ercan B, Balasubramanian K, Webster TJ

International Journal of Nanomedicine 2011, 6:1765-1771

Published Date: 23 August 2011

Selenium nanoparticles inhibit Staphylococcus aureus growth

Tran PA, Webster TJ

International Journal of Nanomedicine 2011, 6:1553-1558

Published Date: 29 July 2011

Reducing infections through nanotechnology and nanoparticles

Taylor E, Webster TJ

International Journal of Nanomedicine 2011, 6:1463-1473

Published Date: 13 July 2011

Self-assembled rosette nanotubes encapsulate and slowly release dexamethasone

Chen Y, Song S, Yan Z, Fenniri H, Webster TJ

International Journal of Nanomedicine 2011, 6:1035-1044

Published Date: 18 May 2011

Silver nanoparticle toxicity in Drosophila: size does matter

Deborah J Gorth, David M Rand, Thomas J Webster

International Journal of Nanomedicine 2011, 6:343-350

Published Date: 11 February 2011

Anodized 20 nm diameter nanotubular titanium for improved bladder stent applications

Ece Alpaslan, Batur Ercan, Thomas J Webster

International Journal of Nanomedicine 2011, 6:219-225

Published Date: 25 January 2011

Self-assembled rosette nanotubes for incorporating hydrophobic drugs in physiological environments

Shang Song, Yupeng Chen, Zhimin Yan, et al

International Journal of Nanomedicine 2011, 6:101-107

Published Date: 10 January 2011

Greater osteoblast and endothelial cell adhesion on nanostructured polyethylene and titanium

Theresa Raimondo, Sabrina Puckett, Thomas J Webster

International Journal of Nanomedicine 2010, 5:647-652

Published Date: 3 September 2010

Differential effects of nanoselenium doping on healthy and cancerous osteoblasts in coculture on titanium

Phong A Tran, Love Sarin, Robert H Hurt, et al

International Journal of Nanomedicine 2010, 5:351-358

Published Date: 10 May 2010

Decreased lung carcinoma cell density on select polymer nanometer surface features for lung replacement therapies

Lijuan Zhang, Young Wook Chun, Thomas J Webster

International Journal of Nanomedicine 2010, 5:269-275

Published Date: 20 April 2010

Bactericidal effect of iron oxide nanoparticles on Staphylococcus aureus

Nhiem Tran, Aparna Mir, Dhriti Mallik, et al

International Journal of Nanomedicine 2010, 5:277-283

Published Date: 14 April 2010

Nanofunctionalized zirconia and barium sulfate particles as bone cement additives

Riaz Gillani, Batur Ercan, Alex Qiao, et al

International Journal of Nanomedicine 2010, 5:1-11

Published Date: 17 December 2009

The use of superparamagnetic nanoparticles for prosthetic biofilm prevention

Erik N Taylor, Thomas J Webster

International Journal of Nanomedicine 2009, 4:145-152

Published Date: 12 August 2009

Enhanced endothelial cell functions on rosette nanotube-coated titanium vascular stents

Eli Fine, Lijie Zhang, Hicham Fenniri, Thomas J Webster

International Journal of Nanomedicine 2009, 4:91-97

Published Date: 17 April 2009

Greater osteoblast proliferation on anodized nanotubular titanium upon electrical stimulation

Batur Ercan, Thomas J Webster

International Journal of Nanomedicine 2008, 3:477-485

Published Date: 5 December 2008

Decreased astroglial cell adhesion and proliferation on zinc oxide nanoparticle polyurethane composites

Justin T Seil, Thomas J Webster

International Journal of Nanomedicine 2008, 3:523-531

Published Date: 5 December 2008

Influence of nanophase titania topography on bacterial attachment and metabolism

Margaret R Park, Michelle K Banks, Bruce Applegate, Thomas J Webster

International Journal of Nanomedicine 2008, 3:497-504

Published Date: 5 December 2008

Enhanced osteoblast adhesion on nanostructured selenium compacts for anti-cancer orthopedic applications

Phong Tran, Thomas J Webster

International Journal of Nanomedicine 2008, 3:391-396

Published Date: 12 September 2008

Biomimetic helical rosette nanotubes and nanocrystalline hydroxyapatite coatings on titanium for improving orthopedic implants

Lijie Zhang, Yupeng Chen, Jose Rodriguez, Hicham Fenniri, Thomas J Webster

International Journal of Nanomedicine 2008, 3:323-333

Published Date: 12 September 2008

Nano rough micron patterned titanium for directing osteoblast morphology and adhesion

Sabrina Puckett, Rajesh Pareta, Thomas J Webster

International Journal of Nanomedicine 2008, 3:229-241

Published Date: 6 June 2008

Enhanced osteoblast adhesion to drug-coated anodized nanotubular titanium surfaces

George E Aninwene II, Chang Yao, Thomas J Webster

International Journal of Nanomedicine 2008, 3:257-264

Published Date: 6 June 2008

Enhanced endothelial cell density on NiTi surfaces with sub-micron to nanometer roughness

Harry D Samaroo, Jing Lu, Thomas J Webster

International Journal of Nanomedicine 2008, 3:75-82

Published Date: 7 March 2008

The influence of nano MgO and BaSO4 particle size additives on properties of PMMA bone cement

Alyssa Ricker, Peishan Liu-Snyder, Thomas J Webster

International Journal of Nanomedicine 2008, 3:125-132

Published Date: 7 March 2008

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