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A novel animal model for residence time evaluation of injectable hyaluronic acid-based fillers using high-frequency ultrasound-based approach

Authors Merola F, Scrima M, Melito C, Iorio A, Pisano C, Giori AM, Ferravante A

Received 10 November 2017

Accepted for publication 23 February 2018

Published 11 July 2018 Volume 2018:11 Pages 339—346

DOI https://doi.org/10.2147/CCID.S156740

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Jeffrey Weinberg


Filomena Merola,1 Mario Scrima,1 Carmela Melito,1 Antonio Iorio,1 Claudio Pisano,2 Andrea Maria Giori,3 Angela Ferravante1

1Research and Development, Bouty SpA, Ariano Irpino, Italy; 2Medicinal Investigational Research, Biogem, Ariano Irpino, Italy; 3Research and Development, IBSA Farmaceutici Italia, Lodi, Italy

Background:
Hyaluronic acid (HA)-based devices are among the most popular filler agents for skin rejuvenation. One of the principal goals is the improvement in residence time of HA-based products, to increase their performance and reduce frequency of the treatment. So, understanding fillers, behavior after subcutaneous injection is a fundamental aspect for discovery and optimization of new products. Current in vivo approaches to detect/quantify injected HA fillers are not always well optimized or easy to apply.
Objective: To develop more efficacious and noninvasive diagnostic tools to make a quantitative evaluation of the degradation of fillers in a small animal model.
Materials and methods: We evaluated the residence time of different HA-based fillers, fluorescein-labeled and not, injected subcutaneously in mice. Volumes of fillers were monitored through high-frequency ultrasound (HF-US) method while fluorescence intensity through the well-established fluorescence living imaging method. To confirm the effectiveness of HF-US, obtained volumetric measurements were compared with fluorescence intensity values.
Results: Both the presented methods revealed the same degradation kinetics for the tested products.
Conclusion: The two used methods are fully comparable and quantitatively accurate. The presented approach has been proved to be noninvasive, sensitive, and reproducible.

Keywords: hyaluronic acid, hyaluronan, dermal filler, HF-US, ultrasound, animal model

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