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A LbL-Assembled Bioactive Coating Modified Nanofibrous Membrane for Rapid Tendon-Bone Healing in ACL Reconstruction

Authors Han F, Zhang P, Chen T, Lin C, Wen X, Zhao P

Received 4 May 2019

Accepted for publication 21 October 2019

Published 25 November 2019 Volume 2019:14 Pages 9159—9172

DOI https://doi.org/10.2147/IJN.S214359

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Dr Mian Wang


Fei Han,1 Peng Zhang,2 Tianwu Chen,2 Chao Lin,1 Xuejun Wen,1 Peng Zhao1

1Institute for Translational Medicine, Institute for Biomedical Engineering and Nanoscience, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200092, People’s Republic of China; 2Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, 200040, People’s Republic of China

Correspondence: Peng Zhao; Peng Zhang
Institute for Translational Medicine, Institute for Biomedical Engineering and Nanoscience, Shanghai East Hospital, Tongji University School of Medicine, Tongji University, Room 222, Shixun Building, Shanghai 200092, People’s Republic of China
Tel +86 21 65988029
Fax +86 21 65983708-0
Email zp@tongji.edu.cn; zhangpengym@163.com

Introduction: In anterior cruciate ligament (ACL) reconstruction, hamstring tendon autograft is a well-accepted surgical choice as an alternative ACL graft. But the main disadvantage of autograft is its inefficient healing with host bone-tunnel which will leading to surgery failure.
Methods: A biomimetic nanofibrous membrane for tendon-bone integration is fabricated in this work, which is composed of polycaprolactone (PCL) electrospinning membrane and chitosan/hyaluronic acid (CS/HA) multilayers film.
Results: By using layer-by-layer (LbL) self-assembly this functional CS/HA multilayer films are deposited on the surface of PCL nanofiber to enable the local delivery of stromal cell-derived factor-1 α (SDF-1α) and bone morphogenetic protein-2 (BMP-2) in tendon-bone interface. This membrane can promote cell proliferation and recruitment, as well as inducing the osteogenic differentiation and recruitment of BMSCs.
Conclusion: Further in vivo studies demonstrate that to wrap the tendon autograft using the membrane may afford superior tendon-bone integration and inhibit scar tissue formation in a rabbit ACL reconstruction model. More importantly, the biomechanical properties of the tendon-bone interface have been improved. This study shows that this biomimetic nanofibrous membrane is effective for improving tendon-bone healing after ACL reconstruction surgery.

Keywords: anterior cruciate ligament reconstruction, tendon-bone healing, layer-by-layer self-assembly, nanofibrous membrane, stromal derived factor-1α


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