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ZIF-8 Modified Polypropylene Membrane: A Biomimetic Cell Culture Platform with a View to the Improvement of Guided Bone Regeneration

Authors Ejeian F, Razmjou A, Nasr-Esfahani MH, Mohammad M, Karamali F, Ebrahimi Warkiani M, Asadnia M, Chen V

Received 5 August 2020

Accepted for publication 26 October 2020

Published 9 December 2020 Volume 2020:15 Pages 10029—10043

DOI https://doi.org/10.2147/IJN.S269169

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Phong A Tran


Fatemeh Ejeian,1,2 Amir Razmjou,1,3 Mohammad Hossein Nasr-Esfahani,2 Munirah Mohammad,3 Fereshteh Karamali,2 Majid Ebrahimi Warkiani,4 Mohsen Asadnia,5 Vicki Chen6

1Department of Biotechnology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan 73441-81746, Iran; 2Department of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran; 3UNESCO Center for Membrane Technology, School of Chemical Engineering, University of New South Wales, Sydney, NSW, 2052, Australia; 4School of Biomedical Engineering, University of Technology Sydney, Sydney, NSW, 2007, Australia; 5School of Engineering, Macquarie University, Sydney, NSW, 2109, Australia; 6School of Chemical Engineering, University of Queensland, Brisbane, QLD, 4072, Australia

Correspondence: Amir Razmjou
UNESCO Center for Membrane Technology, School of Chemical Engineering, University of New South Wales, Sydney, NSW 2052, Australia
Tel +61 (2) 9385 4341
Fax +61 (2) 9385 5966
Email amirr@unsw.edu.au
Mohammad Hossein Nasr-Esfahani Department of Animal Biotechnology, Cell Science Research Center
Royan Institute for Biotechnology, ACECR, Isfahan, Iran
Tel +98 31 95015680
Fax +98 31 95015687
Email mh.nasr-esfahani@royaninstitute.org

Purpose: Despite the significant advances in modeling of biomechanical aspects of cell microenvironment, it remains a major challenge to precisely mimic the physiological condition of the particular cell niche. Here, the metal–organic frameworks (MOFs) have been introduced as a feasible platform for multifactorial control of cell-substrate interaction, given the wide range of physical and mechanical properties of MOF materials and their structural flexibility.
Results: In situ crystallization of zeolitic imidazolate framework-8 (ZIF-8) on the polydopamine (PDA)-modified membrane significantly raised surface energy, wettability, roughness, and stiffness of the substrate. This modulation led to an almost twofold increment in the primary attachment of dental pulp stem cells (DPSCs) compare to conventional plastic culture dishes. The findings indicate that polypropylene (PP) membrane modified by PDA/ZIF-8 coating effectively supports the growth and proliferation of DPSCs at a substantial rate. Further analysis also displayed the exaggerated multilineage differentiation of DPSCs with amplified level of autocrine cell fate determination signals, like BSP1, BMP2, PPARG, FABP4, ACAN, and COL2A. Notably, osteogenic markers were dramatically overexpressed (more than 100-folds rather than tissue culture plate) in response to biomechanical characteristics of the ZIF-8 layer.
Conclusion: Hence, surface modification of cell culture platforms with MOF nanostructures proposed as a powerful nanomedical approach for selectively guiding stem cells for tissue regeneration. In particular, PP/PDA/ZIF-8 membrane presented ideal characteristics for using as a barrier membrane for guided bone regeneration (GBR) in periodontal tissue engineering.

Keywords: metal–organic framework, mesenchymal stem cell, ZIF-8, cell culture platform, barrier membrane

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