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The Effect of Talaromyces marneffei Infection on CD86 Expression in THP-1 Cells

Authors Yang D, Shen L, Chen R, Fu Y, Xu H, Zhang L, Liu D

Received 21 December 2020

Accepted for publication 31 January 2021

Published 19 February 2021 Volume 2021:14 Pages 651—660

DOI https://doi.org/10.2147/IDR.S297160

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Professor Suresh Antony


Di Yang,1,* Lin-xia Shen,1,2,* Ri-feng Chen,1,* Yu Fu,1 Hong-yan Xu,1 Li-na Zhang,1 Dong-hua Liu1,3

1Department of Dermatology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, People’s Republic of China; 2Department of Dermatology and Venereology, Huashan Hospital, Fudan University, Shanghai, 200040, People’s Republic of China; 3Guangxi Key Laboratory of AIDS Prevention and Treatment, School of Public Health, Nanning, 530021, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Dong-hua Liu
Department of Dermatology, The First Affiliated Hospital of Guangxi Medical University, No.6, Shuangyong Road, Qingxiu District, Nanning, Guangxi Province, 530021, People’s Republic of China
Email ldhgxmu@163.com

Background: Talaromyces marneffei (T. marneffei) is a destructive opportunistic dimorphic fungal which can cause lethiferous Talaromycosis, but the clearance of T. marneffei mainly depends on the innate immune response.
Objective: To investigate whether T. marneffei can inhibit the expression of CD86 in THP-1 cells after infection and discuss the potential mechanisms.
Methods: Western blot and immunoelectron microscopy were used to detect the CD86 expression on T. marneffei cultured on BHI medium at 37°C. Western blot, enzyme-linked immunoassay and immunofluorescence were used to detect the change of CD86 expression on macrophages incubating with T. marneffei. Enzyme-linked immunoassay was used to detect the content of CD86 in supernatant in the co-culture system. Immunohistochemistry and immunoelectron microscopy were used to detect the expression of CD86 on T. marneffei incubating with macrophages.
Results: T. marneffei did not express CD86 when cultured separately at 37°C detected by Western blot and immunoelectron microscopy, but it did express CD86 when incubated with macrophages detected by immunohistochemistry and immunoelectron microscopy. The CD86 expression of macrophages significantly decreased at 72 hours when infected with T. marneffei while the content of CD86 in supernatant significantly increased at 72 hours compared with the control group which were detected by Western blot, enzyme-linked immunoassay and immunofluorescence.
Conclusion: 1) After T. marneffei infection, CD86 expression on THP-1 decreased, and with the progression of infection, insufficient polarization of M1 macrophages gradually appeared; 2) T. marneffei may adsorb or uptake CD86 in supernatant produced by macrophages during the contact with THP-1 cells, thus leading to the consumption of CD86 in macrophages.

Keywords: Talaromyces marneffei, T. marneffei, macrophages, CD86, THP-1, immunoelectron microscopy

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