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The anticancer properties of iron core–gold shell nanoparticles in colorectal cancer cells

Authors Wu Y, Wu P, Yang L, Ratinac KR, Thordarson P, Jahn KA, Chen D, Shieh D, Braet F

Received 7 May 2013

Accepted for publication 21 June 2013

Published 2 September 2013 Volume 2013:8(1) Pages 3321—3331

DOI https://doi.org/10.2147/IJN.S47742

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

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Ya-Na Wu,1 Ping-Ching Wu,1 Li-Xing Yang,1 Kyle R Ratinac,2 Pall Thordarson,4 Kristina A Jahn,2 Dong-Hwang Chen,3 Dar-Bin Shieh,1,5,6 Filip Braet2,7

1Institute of Oral Medicine and Department of Stomatology, National Cheng Kung University Hospital, College of Medicine, Tainan, Taiwan; 2Australian Centre for Microscopy and Microanalysis, University of Sydney, Sydney, NSW, Australia; 3Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan; 4School of Chemistry, University of New South Wales, Sydney, NSW, Australia; 5Center for Micro/Nano Science and Technology, National Cheng Kung University, Tainan, Taiwan; 6Advanced Optoelectronic Technology Center, National Cheng Kung University, Tainan, Taiwan; 7School of Medical Sciences (Discipline of Anatomy and Histology), The Bosch Institute, University of Sydney, Sydney, NSW, Australia

Abstract: Previously, iron core–gold shell nanoparticles ([email protected]) have been shown to possess cancer-preferential cytotoxicity in oral and colorectal cancer (CRC) cells. However, CRC cell lines are less sensitive to [email protected] treatment when compared with oral cancer cell lines. In this research, [email protected] are found to decrease the cell viability of CRC cell lines, including Caco-2, HT-29, and SW480, through growth inhibition rather than the induction of cell death. The cytotoxicity induced by [email protected] in CRC cells uses different subcellular pathways to the mitochondria-mediated autophagy found in [email protected] oral cancer cells, OECM1. Interestingly, the Caco-2 cell line shows a similar response to OECM1 cells and is thus more sensitive to [email protected] treatment than the other CRC cell lines studied. We have investigated the underlying cell resistance mechanisms of [email protected] CRC cells. The resistance of CRC cells to [email protected] does not result from the total amount of [email protected] internalized. Instead, the different amounts of Fe and Au internalized appear to determine the different response to treatment with Fe-only nanoparticles in [email protected] CRC cells compared with the [email protected] OECM1 cells. The only moderately cytotoxic effect of [email protected] nanoparticles on CRC cells, when compared to the highly sensitive OECM1 cells, appears to arise from the CRC cells' relative insensitivity to Fe, as is demonstrated by our Fe-only treatments. This is a surprising outcome, given that Fe has thus far been considered to be the "active" component of [email protected] nanoparticles. Instead, we have found that the Au coatings, previously considered only as a passivating coating to protect the Fe cores from oxidation, significantly enhance the cytotoxicity of [email protected] in certain CRC cells. Therefore, we conclude that both the Fe and Au in these core–shell nanoparticles are essential for the anticancer properties observed in CRC cells.

Keywords: cancer therapy, Fe, gold-coated iron, nanoparticles, differential cytotoxicity

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