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Self-assembled albumin nanoparticles for combination therapy in prostate cancer

Authors Lian H, Wu J, Hu Y, Guo H

Received 22 June 2017

Accepted for publication 17 August 2017

Published 24 October 2017 Volume 2017:12 Pages 7777—7787


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Prof. Dr. Dongwoo Khang

Huibo Lian,1 Jinhui Wu,2 Yiqiao Hu,2 Hongqian Guo1

1Department of Urology, Drum Tower Hospital, Medical School of Nanjing University, 2State Key Laboratory of Pharmaceutical Biotechnology, Medical School of Nanjing University, Nanjing, Jiangsu, People’s Republic of China

Abstract: Resistance to regular treatment strategies is a big challenge in the treatment of castration-resistant prostate cancer. Combination of photothermal and photodynamic therapy (PTT/PDT) with chemotherapy offers unique advantages over monotherapy alone. However, free drugs, such as photosensitizers and chemotherapeutic agents, lack tumor-targeted accumulation and can be easily eliminated from the body. Moreover, most of the PTT drugs are hydrophobic and their organic solvents have in vivo toxicity, thereby limiting their potential in clinical translation. Herein, simple multifunctional nanoparticles (NPs) using IR780 (a near-infrared dye) and docetaxel (DTX)-loaded nanoplatform based on human serum albumin (HSA) ([email protected]@DTX) was developed for targeted imaging and for PTT/PDT with chemotherapy for the treatment of castration-resistant prostate cancer treatment. In this platform, HSA is a biocompatible nanocarrier that binds to both DTX and IR780. DTX and IR780, as hydrophobic drug, can induce the self-assembly of HSA proteins. Transmission electron microscopic imaging showed that NPs formed by self-assembly are spherical with a smooth surface with a hydrodynamic diameter of 146.5±10.8 nm. The cytotoxicity of [email protected]@DTX NPs with or without laser irradiation in prostate cancer cells (22RV1) was determined via CCK-8 assay. The antitumor effect of [email protected]@DTX plus laser irradiation was better than either [email protected]@DTX without laser exposure or single PTT heating induced by [email protected] NPs under near-infrared laser, suggesting a significant combined effect in comparison to monotherapy. Near-infrared fluorescence imaging showed that [email protected]@DTX NPs could preferentially accumulate in tumors. In vivo therapeutic efficacy experiment showed that xenografted prostate tumors on mice treated with [email protected]@DTX plus near-infrared laser irradiation were completely inhibited, whereas tumors on mice treated with chemotherapy alone ([email protected] and [email protected]@DTX without laser) or PTT/PDT alone ([email protected] with laser) showed moderate growth inhibition. Overall, [email protected]@DTX NPs showed notable targeting and theranostic potential for the treatment of castration-resistant prostate cancer.

Keywords: prostate cancer, combination therapy, albumin nanoparticles, photothermal and photodynamic therapy, chemotherapy


Prostate cancer is the development of cancer in the prostate, a gland in the male reproductive system. It may initially cause no symptoms; however, in later stages it can lead to difficulty urinating, hematuresis, or pain in the bone. Prostate cancer cells may spread to other parts of the body, particularly to the bones and to the lymph nodes.1 The treatment strategies available for prostate cancer are multiple. The primary treatments for localized prostate cancer include radical prostatectomy and radiotherapy, whereas for advanced stages, treatments such as chemotherapy, androgen deprivation therapy, and adjuvant radiotherapy are considered.2 However, most prostate cancers become treatment-resistant after several cycles of therapy, with poor prognosis and low survival.36 Hence, more effective therapeutic approaches need to be developed in order to improve the treatment and survival of patients with prostate cancer.

Combination therapy is a promising strategy to improve the survival of patients with prostate cancer. Photothermal therapy (PTT) or radiotherapy has been combined with chemotherapy to enhance anticancer efficacy of chemotherapy in a synergistic manner.79 Among various combination therapies, the combination of PTT with chemotherapy has showed a superior treatment effect in case of prostate cancer.10,11 PTT usually utilizes near-infrared (NIR) light-absorbing photosensitizers to damage tumor cells with laser irradiation by heating.12 However, both photosensitizers and chemotherapeutic agents lack tumor-targeted accumulation and can be easily eliminated from the body. Moreover, most PTT agents are hydrophobic, thereby limiting their potential application in clinical practice.

In our previous studies, we developed a simple method to remarkably increase the water solubility of hydrophobic drugs by encapsulating them into human serum albumin (HSA).13,14 In this study, a simple method was used to encapsulate hydrophobic IR780 and docetaxel (DTX) into HSA to form HSA nanoparticles (NPs) ([email protected]@DTX) for the combination of PTT and PDT with chemotherapy for the enhancement of treatment of castration-resistant prostate cancer (Scheme 1). Albumin, the primary composition of serum proteins, is an excellent nanocarrier for drug delivery because it is biocompatible, biodegradable, has low immunogenicity, and has low toxicity.15 DTX, the first-line antitumor chemotherapeutic for prostate cancer, can bind to human serum albumin (HSA) via hydrophobic interactions between the drug molecule and the hydrophobic domain of HSA.16 IR780 iodide, an NIR dye, is a highly hydrophobic agent with specific absorption peak at 780 nm. It has been reported that IR780 could effectively produce heat and generate singlet oxygen after irradiation with 808 nm laser, suggesting that IR780 can be an ideal agent for PTT and PDT.17,18 In our proposed drug delivery system, HSA is a biocompatible and biodegradable carrier platform to deliver DTX, an effective antitumor drug, and IR780, a PTT/PDT agent. Furthermore, IR780 could also serve as a fluorescent imaging probe. DTX is the first-line antitumor chemotherapeutic for castration-resistant prostate cancer. Compared with existing NPs and various formulations used in combination PTT–chemotherapy, our self-assembled albumin NPs of DTX are much easier to form and to have potential clinical use for cancer treatment as Abraxane®. In this study, we developed simple multifunctional nanoparticles (NPs) using IR780 (a near-infrared dye)and docetaxel (DTX)-loaded nanoplatform based on human serum albumin (HSA) ([email protected]@DTX) for targeted imaging and for PTT/PDT with chemotherapy for the treatment of castration-resistant prostate cancer treatment.

Scheme 1 A schematic illustration to show the formation of [email protected]@DTX nanoparticles by self-assembly between HSA, DTX, and IR780.
Abbreviations: HSA, human serum albumin; DTX, docetaxel; PTT, photothermal therapy; PDT, photodynamic therapy; NIR, near-infrared.

Materials and methods


HSA solutions were purchased from Baxter. DTX, IR780, and dichlorodihydrofluorescein diacetate (H2DCFDA) were both purchased from Sigma Aldrich. The cell counting kit-8 (CCK-8) was supplied by Dojindo Laboratories (Tokyo, Japan). The obtained [email protected]@DTX NPs were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), and ultraviolet -visible (UV-Vis) spectrophotometry. It showed simultaneous synergistic PDT/PTT and chemotherapy treatment effect in vitro and in vivo for castration-resistant prostate cancer. Deionized water was used throughout the experiments. We purchased 22RV1 cell lines from the Institute of Biochemistry and Cell Biology, Shanghai Institute of Life Sciences, Chinese Academy of Sciences. All the nude mice were purchased from Yangzhou University Medical Center, and the weight of each mouse was between 20 and 22 g.

Preparation and characterization of [email protected]@DTX NPs

We dissolved 200 mg of HSA in 100 mL of deionized water with constant stirring. Next, 2-mercaptoethanol was added to expose the hydrophobic region of HSA. Then, IR780 and DTX (2 and 30 mg/mL, respectively) were dissolved in dimethylformamide by slow addition, which denatures the protein and stirred until they were completely dissolved. We performed ultrafiltration of the obtained dispersions to remove 2-mercaptoethanol and unbound HSA or DTX (the molecular weight was 60 kDa) using an ultrafiltration membrane with the molecular weight cut-off of ~100 kDa. Then, the solution was filtered through 220 nm filter membrane. Finally, the NP dispersions (namely, [email protected], [email protected], and [email protected]@DTX NPs) were stored at 4°C. The amount of IR780 or DTX in NPs was determined by UV–Vis absorption spectra and HPLC. The morphology and particle size of NPs were analyzed by TEM (Hitachi H-7650). The NPs were dried on a copper grid coated with amorphous carbon and then observed at 200 kV. The NPs were also coated in a cathodic evaporator with a fine gold player and observed on a scanning electron microscope (Hitachi S-3400N). UV–Vis spectra were measured by a UV–Vis spectrophotometer (UV2450, Shimadzu Corporation). The diameter of NPs was measured by a DLS analyzer (Brookhaven Instruments Corporation, NY, USA). The final concentration of IR780 or DTX in the NP solutions was 1 mg/mL and 10 mg/mL, respectively. To evaluate the photothermal effects of NPs in aqueous solution, NPs were exposed to 808 nm wavelength laser irradiation (1 W/cm2) with the illumination direction from the top to the bottom of the cuvette. The negative control was an equivalent amount of water with the same laser irradiation. The temperature of solution was determined with a thermal probe at different time points.

ROS generation by [email protected] and [email protected]@DTX NPs in cancer cells

H2DCFDA acts as a fluorescent probe for ROS in cells, which is non-fluorescent before penetrating into cells. Once entering the cells, the diacetate group of H2DCFDA would be removed by intracellular esterases. The remaining part would be oxidized rapidly and emit green fluorescence in the presence of ROS. Based on this principle, pre-seeded 22RV1 cells in a confocal 12-well plate were co-incubated with [email protected] NPs, [email protected] NPs, [email protected]@DTX NPs dispersions, or with PBS and H2DCFDA (40 μM) for 4 h in an atmosphere of 37°C and 5% CO2. Subsequently, the cells were washed with phosphate buffer saline (PBS) and the central region in the 12-well plate was exposed to 808 nm wavelength laser irradiation (1 W/m2) for 5 min. Fluorescence images in the irradiation region were immediately captured on a confocal fluorescence microscope (Olympus FV1000) using an excitation of 504 nm and an emission of 510–560 nm.

Cellular uptake of [email protected] NPs and [email protected]@DTX NPs

We seeded 22RV1 cells onto a glass-bottom dish (glass diameter 10 mm) at a density of 1.0×104 cells/dish and cultured for 24 h in an atmosphere of 37°C in 5% humidified CO2. After 24 h, the medium was changed to the fresh medium containing [email protected] and [email protected]@DTX NPs. After 2 h incubation, the cells were washed thrice with PBS and fixed with 4% paraformaldehyde solution for 20 min, and then stained with DAPI for 15 min. Finally, the cells were observed by confocal laser scanning microscope (CLSM, Leica TCS SP5, Germany). The excitation wavelength of IR780 was 633 nm and the emission spectrum recorded was between 700 and 800 nm. In addition, for the cellular uptake experiment by flow cytometry, 22RV1 cells were seeded in 6-well plate at a density of 5×105/well. The treatment was the same as cell uptake mentioned above. Then, the cells were washed with PBS three times, centrifuged, re-suspended in 500 μL PBS, and analyzed by flow cytometry. The fluorescence of IR780 was collected on the FL4-H channel.

In vitro therapeutic efficacy of NPs

The cytotoxicity of [email protected] and [email protected]@DTX NPs with or without light irradiation in 22RV1 cells was determined via CCK-8 assay. Briefly, the cells were seeded in a 96-well plate at a density of 5×103 cells/well in 100 μL DMEM containing 10% FBS. After incubation for 24 h, samples with various concentrations of NPs were added to each well. After 2 h, the medium was replaced with 100 μL fresh medium, and the cells were irradiated with a 1 W/cm 2 808-nm laser for 2.5 min. Control cells were treated identically but not irradiated with the laser. Subsequently, the cells were incubated for 24 h, and CCK-8 assay was performed to quantify the cell viability. The cell viability was defined as the percentage of surviving cells versus untreated cells.

In vivo NIR fluorescence imaging of [email protected]@DTX NPs

In vivo imaging and biodistribution analysis

When the size of the tumor in mice reached about 100–200 mm3, the [email protected]@DTX NPs (3 mg IR780/kg body weight) were injected via tail vein (n=6). Images were taken at 0, 12, 24, and 48 h after injection using the in vivo imaging system (IVIS Lumina XR III, USA). Three of the BALB/c mice were sacrificed at 48 h after injection. Then the organs including heart, liver, spleen, lung, kidney, brain, and tumor tissue were collected for imaging and semi-quantitative biodistribution analysis by the imaging system. The excitation wavelength employed for IR780 was 735 nm and the emission spectrum was at 780–900 nm.

Therapeutic efficacy of NPs in vivo

Male nude mice received care in compliance with the guidelines outlined in the Guide for the Care and Use of Laboratory Animals and were used in accordance with the regulations of the Institutional Animal Care and Use Committee (IACUC) of Nanjing University. All animal tests and experimental procedures were approved by the Administration Committee of Experimental Animals in Jiangsu Province and the Ethics Committee of Nanjing University.

To establish the tumor model, 22RV1 cells (5×106) were administered via subcutaneous injection into the right flank of the mice. Treatments were initiated when the tumor volumes reached 100–200 mm3. To evaluate the thermal effect of NPs in vivo, 100 μL of [email protected] and [email protected]@DTX NPs (5 mg/kg, concentration of IR780) was injected into the tumor-bearing mice by vein. After 24 h, an infrared (IR) thermal camera (Fluke, USA) was used to monitor the temperature change when the tumors were exposed to 808 nm laser power density of 1 W/cm2 at 1 min intervals for a total of 4 min. When the tumor volumes reached about 100–200 mm3, the mice were divided into seven groups and treated with PBS, [email protected], [email protected], or [email protected]@DTX with or without laser irradiation (IR780 dose: 5 mg/kg). All samples were given by intravenous injection via tail vein. The day of administration was set as day 0 and 24 h later, the tumors were exposed to 808 nm laser (1 W/cm 2) for 4 min. Tumor volumes were calculated using the following formula: tumor volume = length × width 2×0.5. Tumor sizes and body weights were measured every 3 days for the duration of the experiment. After more than 2 weeks of treatment, the animals were sacrificed and tumors and primary organs (heart, liver, spleen, lung, and kidney) were harvested for H&E staining.

Results and discussion

Preparation and characterization of [email protected]@DTX NPs

[email protected], [email protected] NPs, and [email protected]@DTX NPs were prepared according to the previous methods.13 First, the disulfide bond of has was broken by 2-mercaptoenthanol and the hydrophobic domains of HSA were exposed. IR780 and DTX are strong hydrophobic molecules, tending to integrate with the exposed hydrophobic parts of HSA to form the NPs. While the hydrophilic groups of HSA were exposed to water, the mixture of IR780, DTX, IR780, and DTX were enclosed in the hydrophobic groups of HSA. Then, the [email protected], [email protected] NPs, and [email protected]@DTX NPs were formed. The optimized formulation prepared at a molar ratio of HSA:IR780 (1:10), HSA:DTX (1:30), and HSA:IR780:DTX (1:10:30) were selected and used in our following experiments. The obtained NP dispersions were ultrafiltered to remove excess molecules. The IR780 and DTX content in the [email protected]@DTX formulation were measured to be 2.3% and 6.5% (the loading efficiencies of IR780 and DTX were 76.9% and 39.4%), respectively, by high-performance liquid chromatography (HPLC).

We characterized the obtained [email protected], [email protected] NPs, and [email protected]@DTX NPs. TEM imaging showed that all NPs formed by self-assembly were spherical with a smooth surface (Figure 1A). Hydrodynamic diameters of [email protected], [email protected] NPs, and [email protected]@DTX were measured by DLS. The mean diameter was found to be 142±10.5, 170.1±17.9, and 146.5±10.8, respectively (Figure 1B). In the preparation of different kinds of NPs, HSA as a nanocarrier was excess for [email protected] NPs and [email protected]@DTX NPs; thus, when more hydrophobic drugs were encapsulated in HSA, it may produce more NPs rather than increase particle size. As shown in Figure 1C, UV–Vis spectra indicated that HSA-DTX solution had no absorption in the NIR region. Compared with free IR780, [email protected] and [email protected]@DTX showed slightly red-shifted absorbance peak probably due to the molecular conformation change after IR780 is complexed with the albumin. Next, we investigated the photothermal profile of the NPs. [email protected] and [email protected]@DTX solutions showed the similar photothermal profile under the 808 nm laser irradiation (1 W/cm2) (Figure 1D). The temperature change of NPs was in a concentration-dependent manner. The temperature of the [email protected]@DTX NPs (20 μg/mL) increased from room temperature (26.4°C) to 45°C in 90 s under laser irradiation. The maximum temperature was found to be 47.5°C. Such observed temperatures are high enough to cause irreversible hyperthermia damage to tumor cells. To verify the photodynamic effects of NPs, the singlet oxygen generation of NPs is manifested by the fluorescence intensity of singlet oxygen sensor green (SOSG). The fluorescence intensity of NPs gradually increased after being exposed to 808 nm laser irradiation, indicating the generation of singlet oxygen generated (Figure 1E). The release profiles of DTX from [email protected]@DTX nanocomplexes with or without NIR laser irradiation were also investigated (Figure 1F). As shown in Figure 1, there was no significant difference between the release profile with NIR laser irradiation and without NIR laser irradiation.

Figure 1 Preparation and characterization of HSA–ICG–DTX complex. (A) TEM images of [email protected], [email protected], and [email protected]@DTX. (B) Hydrodynamic diameters of [email protected], [email protected], and [email protected]@DTX measured by DLS. (C) UV–Vis–NIR spectra of Free IR780, [email protected], [email protected], and [email protected]@DTX. (D) Temperature change of PBS, [email protected], and [email protected]@DTX in the irradiation of a NIR laser (808 nm) with a power density of 1 W/cm2. (E) Singlet oxygen generation of NPs after NIR laser irradiation. (F) Release profiles of [email protected]@DTX with or without laser irradiation.
Abbreviations: DTX, docetaxel; FL, fluorescence; HSA, human serum albumin; NIR, near-infrared; PBS, phosphate buffer saline; SOSG, singlet oxygen sensor green; ICG, indocyanine green.

ROS generation of [email protected] and [email protected]@DTX NPs in cancer cells

We studied ROS generation under exposure to 808 nm laser irradiation. ROS generation induced by IR780 can oxidize H2DCFDA to produce green fluorescence. As shown in Figure 2, fluorescence can be barely observed in cells treated with [email protected] and [email protected]@DTX NPs without laser irradiation. However, cells treated with [email protected] and [email protected]@DTX NPs plus NIR irradiation exhibited obvious green fluorescence, demonstrating an amount of ROS generation in cells and further predicting the application of NPs for PDT therapy.

Figure 2 Confocal fluorescence images of ROS generation in MCF-7 cells. From top to bottom: [email protected] NPs, [email protected] NPs plus NIR laser (808 nm, 1 W/cm2, 2 min), [email protected]@DTX NPs and [email protected]@DTX NPs plus NIR laser. The final concentration of [email protected] NPs (calculated as IR780) was 5 μg/mL. The green fluorescence color represents oxidatively stressed cells affected with ROS. Fluorescence images were collected at 510~560 nm, under an excitation at 504 nm. The scale bar is 20 μm.
Abbreviations: DTX, docetaxel; HSA, human serum albumin; NIR, near-infrared; NPs, nanoparticles.

Cell uptake of [email protected] NPs and [email protected]@DTX NPs

The cellular uptake of [email protected] and [email protected]@DTX NPs in 22RV1 cells were confirmed by a confocal fluorescence microscopy. After 2 h incubation, strong red fluorescence was observed in the cytoplasm of the cells treated with [email protected] and [email protected]@DTX NPs, suggesting that [email protected] and [email protected]@DTX NPs are taken up by the malignant cells (Figure 3A). Flow cytometric analysis (Figure 3B) confirmed that [email protected] NPs and [email protected]@DTX NPs are taken up by cells in equal quantities. Our results indicate that DTX did not influence the uptake of [email protected]@DTX NPs.

Figure 3 Cellular uptake of [email protected] NPs and [email protected]@DTX NPs. (A) Confocal fluorescence images of 22RV1 cells localization of [email protected] NPs and [email protected]@DTX NPs 45 min post-incubation. Blue fluorescence indicates positive staining of Hochest and red fluorescence is the fluorescence of IR780. (B) Flow cytometry analysis of 22RV1 cell line incubated with PBS, [email protected] NPs, and [email protected]@DTX NPs, respectively.
Abbreviations: DTX, docetaxel; HSA, human serum albumin; NPs, nanoparticles; PBS, phosphate buffer saline.

In vitro antitumor therapeutic efficacy of the NPs

Next, we tested the in vitro antitumor efficacy of [email protected], [email protected] NPs, and [email protected]@DTX NPs in a cancer cell model. Briefly, 22RV1 human prostate cancer cells, cultured under recommended conditions, were firstly incubated with [email protected], [email protected] NPs, and [email protected]@DTX NPs for 3 h, then washed with PBS to remove free NPs. Cells were irradiated with 808 nm laser at a power density of 1 W cm2 for 2.5 min. After 24 h, the cell viabilities were determined by the CCK-8 assay to determine the antitumor therapeutic efficacy of the NPs. As shown in Figure 4, the cell viability of cells treated with [email protected]@DTX plus laser irradiation was much lower than that of cells treated with [email protected]@DTX without laser irradiation, [email protected] with or without laser irradiation, or single photothermal heating induced by [email protected] NPs under NIR laser, suggesting a significant combination effect in comparison to monotherapy. Moreover, [email protected] NPs seem to be affecting cell viability at 1 and 1.5 μg/mL concentrations, probably because IR780 have dark toxicity due to its mitochondria-targeted and hydrophobic property.13,19

Figure 4 In vitro cell experiments. Relative viabilities of 22RV1 cells after incubation with different concentrations of IR780 and DTX in [email protected], [email protected] NPs, and [email protected]@DTX NPs with or without 808 nm laser irradiation (1 W/cm2, 2 min).
Abbreviations: DTX, docetaxel; HSA, human serum albumin; NPs, nanoparticles; NIR, near-infrared.

In vivo imaging of [email protected]@DTX NPs

To evaluate the feasibility of [email protected]@DTX NPs as an ideal nano-photosensitizer, in vivo NIR fluorescence imaging was performed on 22RV1 tumor-bearing mice. NIR fluorescence imaging was applied on tumor-bearing mice which were injected with [email protected]@DTX NPs via the tail vein. As shown in Figure 5A, [email protected]@DTX NPs tended to accumulate in the tumor over time, with prominent accumulation in tumor 48 h post-injection. To further confirm the accumulation of the NPs in tumor, fluorescence in tumor and organs was detected 48 h after injection of the NPs. The results revealed that the tumor had stronger fluorescent signals, confirming the accumulation of the NPs (Figure 5B). The preferential accumulation in tumors might be due to the enhanced permeability and retention effects.

Figure 5 NIR imaging and biodistribution analysis. (A) The fluorescence images of mice with prostate cancer injected with NPs (the white arrows indicated tumor area). (B) Ex vivo fluorescence images of major organs from those mice at 48 h. (C) Semiquantitative biodistribution of NPs in major organs. The data are shown as mean ± SD (n=3).
Abbreviations: NIR, near-infrared; NPs, nanoparticles.

Therapeutic efficacy of NPs in vivo

Motivated by the high tumor accumulation of [email protected]@DTX, we next investigated the in vivo combined PTT with chemotherapy using the 22RV1 subcutaneous tumor model in mice. Seven groups of 22RV1 tumor-bearing mice with five mice per group were used in this experiment. When the tumor volume reached 100–200 mm3, PBS, [email protected], [email protected], or [email protected]@DTX solutions were injected into the mice intravenously (doses: IR780 5 mg/kg, DTX 20 mg/kg). The tumors were then irradiated by the 808 nm laser at a power density of 1 W/cm2 for 4 min 24 h post-injection when the tumor accumulation of [email protected]@DTX reached its peaked level. An IR thermal camera was used to monitor the temperature change of tumor during laser irradiation. For mice intravenously injected with [email protected] or [email protected]@DTX, the tumor temperatures rapidly increased to ~53°C under the laser exposure, whereas mice with PBS injection did not show any significant change under same laser exposure (Figure 6A). However, even though PTT alone induced tumor damage, it could not completely destruct subcutaneous 4T1 tumors based on previous reports.20 The tumor sizes were then measured by a caliper. At 2 days post-injection, [email protected]@DTX plus laser and [email protected] plus laser-treated mice demonstrated a severe tumor necrosis at the site of the laser irradiation. A similar necrosis was not observed in the other groups (Figure 7A). After 9 days, it was apparent that the necrotic scar tissue was healed and that normal tissue had begun to regenerate in the [email protected]@DTX plus laser-treated mice. The therapeutic efficacy of each treatment was monitored by evaluating the tumor volumes for 18 days (Figure 6B). Remarkably, tumors on mice treated with [email protected]@DTX plus NIR laser irradiation were completely inhibited without a single case of regrowth in the period up to 18 days. In contrast, tumors on mice with chemotherapy alone ([email protected], [email protected]@DTX without laser) or with PTT alone ([email protected] with laser) showed a moderate growth inhibition effect. Particularly, tumor size in the [email protected] with laser group was similar to that in [email protected]@DTX with laser group in the first 3 days after the treatment. However, 3 days later, the [email protected] with laser group showed tumor regrowth gradually and [email protected]@DTX with laser group showed continuous tumor growth inhibition effect, owing to the combination effect of PTT and chemotherapy. H&E staining of tumor slices collected 18 days post-treatment revealed that tumor cells were severely destroyed after combination therapy ([email protected]@DTX plus NIR laser), while those after single therapy were only partially damaged (Figure 7B). Neither body weight loss nor animal death was observed in each experimental group (Figure 6C). H&E stained images of the primary organs from [email protected]@DTX-injected mice 18 days post-injection indicated no appreciable abnormality or noticeable organ damage (Figure 7C). Therefore, our results demonstrate an excellent synergistic therapeutic effect of the combined PTT and chemotherapy delivered by [email protected]@DTX, which did not show significant toxic side effect on the treated animals.

Figure 6 Imaging guided combination therapy on a subcutaneous tumor model. (A) Heating curves of tumor-bearing mice with PBS, [email protected] NPs and [email protected]@DTX NPs. Data are expressed as mean ± SD (n=3). (B) The tumor growth curves of different group of mice after various treatments indicated (five mice per group). Error bars were based on standard errors of the mean. (C) Body weight of mice in different group after treatment.
Abbreviations: DTX, docetaxel; HSA, human serum albumin; NPs, nanoparticles; PBS, phosphate buffer saline.

Figure 7 Therapeutic efficacy and biosafety evaluation of formed nanoparticles in vivo.
Notes: (A) Representative images of mice bearing prostate cancer pre-treatment and after treatments, (B) the corresponding H&E-stained tumor sections, (C) H&E staining of major organs after the treatment of nanoparticles.
Abbreviations: DTX, docetaxel; HSA, human serum albumin; NIR, near-infrared; PBS, phosphate buffer saline.


We developed a simple method to encapsulate hydrophobic IR780 and DTX into HSA to form multifunctional [email protected]@DTX NPs for the combination therapy of PTT and PDT with chemotherapy in the treatment of castration-resistant prostate cancer. These new [email protected]@DTX NPs exhibit great advantages in both imaging and combined therapy, suggesting a great potential in clinical translation. Based on such biocompatible theranostic agent, it is hoped that imaging-guided combined PTT and/or PDT therapy with chemotherapy would bring new perspectives to the future fight against castration-resistant prostate cancer.


This work was supported by grants from the National Natural Science Foundation of China (Nos 81572519, 81502203, 81602221) and Jiangsu Provincial Medical Youth Talent (QNRC2016017).


The authors report no conflicts of interest in this work.



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