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rs187960998 polymorphism in miR-211 prevent development of human colon cancer by deregulation of 3’UTR in CHD5

Authors Zhu L, Wang R, Zhang L, Zuo C, Zhang R, Zhao S

Received 20 July 2018

Accepted for publication 16 November 2018

Published 3 January 2019 Volume 2019:12 Pages 405—412


Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Andrew Yee

Peer reviewer comments 3

Editor who approved publication: Dr Sanjeev Srivastava

Limei Zhu,* Ran Wang,* Li Zhang, Chunlei Zuo, Rui Zhang, Shaolin Zhao

Department of Clinical Laboratory, The First People’s Hospital of Lianyungang, Lianyungang, Jiangsu, China

*These authors contributed equally to this work

Background: Previous research indicated that overexpression of miRNA-211 could promote colorectal cancer cell growth by targeting tumor suppressive gene Chromodomain-helicase-DNA-binding protein 5 (CHD5) in human colon cancer (CC). Moreover, the function of the single-nucleotide polymorphism (SNP) located in the mature region of miR-211 has not been investigated. In this study, we found that SNP of rs187960998 in miR-211 was involved in the occurrence of CC by acting as a tumor suppressor by mal-regulation of its target gene CHD5.
Materials and methods: The genotype of total 685 CC patients was detected by real-time PCR, the proliferation of CC cell lines with different genotypes of miR-211 was determined by Cell Counting Kit-8, cell invasion evaluated by transwell and the activity of the CHD5 promoter in CC cell lines transfected with different miR-211 was determined by luciferase assay. The expression of CHD5 in CC patients was determined by the immunohistochemistry, and the relapse-free survival rate was analyzed by Kaplan–Meier analysis.
Results: C/T SNP of miR-211 could inhibit CC cell proliferation and invasion by upregulation of CHD5. And SNP in rs187960998 of miR-211 was associated with tumor size, metastasis and tumor differentiation in CC patients. Patients with CC genotype have significantly low CHD5 expression than the T-carrier, while no significant expression difference in miR-211 expression among different genotype subsets. Patients with CC genotype have significantly shorter postsurgery survival rate compared to the T-carrier.
Conclusion: rs187960998 in miR-211 was highly associated with a decreased risk of CC in the Chinese population by deregulating a tumor suppressive gene CHD5.

Keywords: miR-211, colon cancer, CHD5, SNP, survival

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