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Role of microparticles derived from monocytes, endothelial cells and platelets in the exacerbation of COPD

Authors Tőkés-Füzesi M, Ruzsics I, Rideg O, Kustán P, Kovács GL, Molnár T

Received 29 May 2018

Accepted for publication 3 September 2018

Published 15 November 2018 Volume 2018:13 Pages 3749—3757

DOI https://doi.org/10.2147/COPD.S175607

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Ms Justinn Cochran

Peer reviewer comments 3

Editor who approved publication: Dr Richard Russell


Margit Tőkés-Füzesi,1 István Ruzsics,2 Orsolya Rideg,3 Péter Kustán,1 Gábor L Kovács,1,4 Tihamér Molnár5

1Department of Laboratory Medicine, University of Pécs, Medical School, Pécs, Hungary; 21st Department of Internal Medicine, Division of Pulmonology, University of Pécs, Medical School, Pécs, Hungary; 3Department of Pharmacy, University of Pécs, Pécs, Hungary; 4Szentágothai Research Centre, Pécs, Hungary; 5Department of Anesthesiology and Intensive Therapy, University of Pécs, Medical School, Pécs, Hungary

Background: Microparticles (MPs) are shedding membrane vesicles released from activated blood and endothelial cells under inflammatory conditions. The role of endothelial MPs (EMPs) in pathophysiology of COPD is relatively well known. However, the release and function of MPs of other cellular origins, eg, platelets, red blood cells and leukocytes, are not clearly evaluated in COPD.
Purpose: The aim of this study was to measure EMPs and other cell-derived circulating MPs in stable and exacerbated COPD patients.
Patients and methods: A total of 50 patients with COPD and 19 healthy volunteers were enrolled in the study. EMPs (CD31+, CD62E+) and platelet-derived (CD61+, CD41+, CD42a+, PAC1+), red blood cell-derived (GlyA+) and leukocyte-derived (CD45+, CD13+, CD14+, CD56+) MPs were measured. Flow cytometry (FC) was performed on Beckman Coulter FC500 analyzer. MP reference gate was set using 0.3–0.5–0.9 µm microbeads with MP size gates of 0.5–1.0 µm.
Results: All the measured MPs were significantly (P<0.001) higher in COPD patients than in the controls. Furthermore, CD62E+, CD41+, CD42a+ and CD14+ MP values were significantly (P<0.001) increased in exacerbated COPD compared to stable COPD. These MPs showed significant (P<0.001) inverse correlation with FEV1/FVC, as well.
Conclusion: In this study, we describe a reliable flow cytometric assay for MP analysis that was successfully applied in COPD. Besides EMPs, COPD is accompanied by an increased concentration of various MPs in the systemic circulation; particularly, platelet- and monocyte-derived MPs seem to be important in exacerbation.

Keywords: cell-derived microparticles, biomarker, COPD, flow cytometry

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