Quantitative and multiplexed detection for blood typing based on quantum dot–magnetic bead assay
Authors Xu T, Zhang Q, Fan YH, Li RQ, Lu H, Zhao SM, Jiang TL
Received 25 January 2017
Accepted for publication 23 March 2017
Published 26 April 2017 Volume 2017:12 Pages 3347—3356
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Jiang Yang
Peer reviewer comments 3
Editor who approved publication: Dr Linlin Sun
Ting Xu, Qiang Zhang, Ya-han Fan, Ru-qing Li, Hua Lu, Shu-ming Zhao, Tian-lun Jiang
Department of Transfusion Medicine, Southwest Hospital, Third Military Medical University, Chongqing, People’s Republic of China
Background: Accurate and reliable blood grouping is essential for safe blood transfusion. However, conventional methods are qualitative and use only single-antigen detection. We overcame these limitations by developing a simple, quantitative, and multiplexed detection method for blood grouping using quantum dots (QDs) and magnetic beads.
Methods: In the QD fluorescence assay (QFA), blood group A and B antigens were quantified using QD labeling and magnetic beads, and the blood groups were identified according to the R value (the value was calculated with the fluorescence intensity from dual QD labeling) of A and B antigens. The optimized performance of QFA was established by blood typing 791 clinical samples.
Results: Quantitative and multiplexed detection for blood group antigens can be completed within 35 min with more than 105 red blood cells. When conditions are optimized, the assay performance is satisfactory for weak samples. The coefficients of variation between and within days were less than 10% and the reproducibility was good. The ABO blood groups of 791 clinical samples were identified by QFA, and the accuracy obtained was 100% compared with the tube test. Receiver-operating characteristic curves revealed that the QFA has high sensitivity and specificity toward clinical samples, and the cutoff points of the R value of A and B antigens were 1.483 and 1.576, respectively.
Conclusion: In this study, we reported a novel quantitative and multiplexed method for the identification of ABO blood groups and presented an effective alternative for quantitative blood typing. This method can be used as an effective tool to improve blood typing and further guarantee clinical transfusion safety.
Keywords: blood typing, quantum dots, magnetic beads, blood group antigens, fluorescence detection
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