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Polo-like kinase 3 is associated with poor prognosis and regulates proliferation and metastasis in prostate cancer

Authors Lin C, Bai S, Du T, Lai Y, Chen X, Peng S, Ma X, Wu W, Guo Z, Huang H

Received 9 June 2018

Accepted for publication 6 October 2018

Published 14 February 2019 Volume 2019:11 Pages 1517—1524

DOI https://doi.org/10.2147/CMAR.S176762

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Justinn Cochran

Peer reviewer comments 3

Editor who approved publication: Dr Antonella D'Anneo


Chunhao Lin,1,2,* Shoumin Bai,1,3,* Tao Du,4 Yiming Lai,2 Xianju Chen,5 Shengmeng Peng,2 Xiaoming Ma,2 Wanhua Wu,2 Zhenghui Guo,1,2 Hai Huang1,2

1Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China; 2Department of Urology, Sun Yatsen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China; 3Department of Radiation Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China; 4Department of Obstetrics and Gynecology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China; 5Department of Urology, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518017, China

*These authors contributed equally to this work

Background: Biological mechanism of prostate cancer (PCa) recurrence and progress is complex but many of the key elements are not fully understood. Polo-like kinases (Plks) represent a family of highly conserved serine–threonine kinases that play essential roles in cell cycle progression. Plk3 plays contradictory roles in different cancers. However, the roles of Plk3 in PCa remain largely unexplored.
Methods: Kaplan–Meier analysis and Cox regression analysis were performed to evaluate the relationship between Plk3 and prognosis of patients with PCa. Gene set enrichment analysis (GSEA) was conducted to evaluate proliferation and metastasis gene sets using The Cancer Genome Atlas Dataset. MTS assay, clone formation assay, cell migration, and wound healing assay were carried out to investigate biological functions of Plk3.
Results: We found that high Plk3 expression was closely correlated with poor prognosis. GSEA revealed that Plk3 was involved in proliferation and metastasis. Loss-of-function assays demonstrated that Plk3 promoted proliferation and metastasis in PCa cells in vitro.
Conclusion: We discovered that Plk3 plays a critical role in PCa, indicating that it may be a potential prognostic marker and help predict the progression, especially recurrence of PCa.

Keywords: prostate cancer, polo-like kinase 3, recurrence, proliferation, migration, GSEA


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