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Over-expression of nuclear factor-κB family genes and inflammatory molecules is related to chronic obstructive pulmonary disease

Authors Zhou L, Liu Y, Chen X, Wang S, Liu H, Zhang T, Zhang Y, Xu Q, Han X, Zhao Y, Song X, Ye L

Received 30 January 2018

Accepted for publication 11 May 2018

Published 12 July 2018 Volume 2018:13 Pages 2131—2138

DOI https://doi.org/10.2147/COPD.S164151

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Professor Hsiao-Chi Chuang

Peer reviewer comments 2

Editor who approved publication: Professor Chunxue Bai


Liting Zhou,1 Ying Liu,2 Xin Chen,1 Shuyue Wang,3 Hongbo Liu,1 Tianrong Zhang,1 Yuezhu Zhang,1 Qi Xu,1 Xu Han,1 Yaming Zhao,1 Xinyue Song,1 Lin Ye1

1Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, China; 2Department of Respiratory Medicine, The First Hospital of Jilin University, Jilin University, Changchun, China; 3Department of Emergency, China-Japan Union Hospital, Jilin University, Changchun, China

Background: Nuclear factor-κB (NF-κB) signaling plays essential roles in inflammatory responses. However, whether the expression levels of NF-κB family genes affect inflammatory responses is unclear. Moreover, little is known regarding the association between NF-κB family genes expression and the pathogenesis of chronic obstructive pulmonary disease (COPD). The present study was undertaken to assess the relationship between the expression levels of NF-κB family genes mRNA and of inflammatory markers relevant to COPD pathogenesis.
Methods: A total of 186 unrelated patients with acute exacerbations of COPD and 180 healthy controls were recruited. Total RNA was extracted from the peripheral fasting blood of each subject using trizol reagent. The mRNA levels of NF-κB family genes (NF-κB1, NF-κB2 and c-REL) were measured by real-time quantitative polymerase chain reaction. The serum levels of cyclooxygenase-2 (COX-2), C-reactive protein, interleukin (IL)-1β, IL-6, IL-8, IL-12 and tumor necrosis factor-α were measured with enzyme-linked immunosorbent assay kits.
Results: The relative mRNA levels of the NF-κB family genes and the levels of inflammatory molecules were significantly higher in the COPD group than in the control group after adjustment for smoking. The IL-1β, IL-8 and COX-2 levels were significantly lower in the NF-κB2 under-expression subgroup as compared to the NF-κB2 over-expression subgroup. The COX-2 level was significantly lower (P < 0.05) in the c-REL under-expression subgroup as compared to the c-REL over-expression subgroup.
Conclusion: NF-κB2 over-expression was associated with IL-1β, IL-8 and COX-2 levels, whereas c-REL over-expression was associated with COX-2 level. Over-expression of both NF-κB2 and c-REL was found to be related to COPD.

Keywords: chronic obstructive pulmonary disease, inflammation, NF-κB, gene expression

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