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OCT corneal epithelial topographic asymmetry as a sensitive diagnostic tool for early and advancing keratoconus

Authors Kanellopoulos AJ, Asimellis G

Received 17 May 2014

Accepted for publication 19 June 2014

Published 18 November 2014 Volume 2014:8 Pages 2277—2287


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 5

Anastasios John Kanellopoulos,1,2 George Asimellis1 Eye Institute, Athens, Greece; 2Department of Ophthalmology, New York University Medical School, New York, NY, USA

Purpose: To investigate epithelial thickness-distribution characteristics in a large group of keratoconic patients and their correlation to normal eyes employing anterior-segment optical coherence tomography (AS-OCT).
Materials and methods: The study group (n=160 eyes) consisted of clinically diagnosed keratoconus eyes; the control group (n=160) consisted of nonkeratoconic eyes. Three separate, three-dimensional epithelial thickness maps were obtained employing AS-OCT, enabling investigation of the pupil center, average, mid-peripheral, superior, inferior, maximum, minimum, and topographic epithelial thickness variability. Intraindividual repeatability of measurements was assessed. We introduced correlation of the epithelial data via newly defined indices. The epithelial thickness indices were then correlated with two Scheimpflug imaging-derived AS-irregularity indices: the index of height decentration, and the index of surface variance highly sensitive to early and advancing keratoconus diagnosis as validation.
Results: Intraindividual repeatability of epithelial thickness measurement in the keratoconic group was on average 1.67 µm. For the control group, repeatability was on average 1.13 µm. In the keratoconic group, pupil-center epithelial thickness was 51.75±7.02 µm, while maximum and minimum epithelial thickness were 63.54±8.85 µm and 40.73±8.51 µm. In the control group, epithelial thickness at the center was 52.54±3.23 µm, with maximum 55.33±3.27 µm and minimum 48.50±3.98 µm epithelial thickness. Topographic variability was 6.07±3.55 µm in the keratoconic group, while for the control group it was 1.59±0.79 µm. In keratoconus, topographic epithelial thickness change from normal, correlated tightly with the topometric asymmetry indices of IHD and ISV derived from Scheimpflug imaging.
Conclusion: Simple, OCT-derived epithelial mapping, appears to have critical potential in early and advancing keratoconus diagnosis, confirmed with its correlation with established Scheimpflug-derived asymmetry topometric indices.

anterior-segment optical coherence tomography, early and advancing keratoconus, epithelial thickness imaging, novel corneal epithelial thickness-asymmetry indices, three-dimensional mapping, Scheimpflug imaging based Pentacam, topographic keratoconus grading, index of surface variance, index of height decentration


There is an increasing awareness pertaining to epithelial thickness characteristics in keratoconus (KCN).13 Specifically, overall corneal epithelial thickness,4,5 as well as epithelial thickness topographic variability, may serve as diagnostic elements, in synergy with other topographic and Scheimpflug tomographic screening indicators, such as irregular corneal thickness6,7 and anterior-surface topographic variability.8,9

In addition, the corneal epithelial layer-thickness distribution can be very useful in clinical10 as well as in basic research.11,12 We have introduced10 and reported13 a potentially novel epithelial clinical indicator for biomechanical corneal instability in keratoconus (KCN) as a criterion demonstrating increased overall corneal epithelial thickness. We have thus proposed this simple clinical parameter as an early keratectasia diagnostic marker.

The recent popularity of clinical in-vivo epithelial mapping by anterior-segment optical coherence tomography (AS-OCT) presents a new and potentially more practical tool, with the speed of optical imaging and noncontact-mode ease of use compared to the previous gold standard for epithelial imaging, high frequency ultrasound.1416

This work aims to investigate three-dimensional epithelial layer thickness characteristics in a large pool of keratoconic patients via clinically available AS-OCT. Keratoconic investigation and severity stratification was facilitated by Scheimpflug imaging; OCT-derived epithelial thickness characteristics were correlated on one-to-one basis with Scheimpflug-derived KCN classification and AS-irregularity indices, which have been previously identified and reported as robust indicators for KCN severity and progression.8

Materials and methods

This comparative case-series study received approval by the ethics committee of our institution, adherent to the tenets of the Declaration of Helsinki. Informed written consent was obtained from each subject at the time of the first clinical visit.

Patient inclusion/exclusion criteria

Study group A (n=160 different eyes) consisted of cases with keratoconic eyes, confirmed by a complete ophthalmologic evaluation and corneal imaging, in which no previous surgery had been applied. KCN diagnosis was established by slit-lamp biomicroscopy for clinical signs of KCN, such as the pattern of opposing central and peripheral retinoscopic movements, the conical reflection on the nasal cornea under temporal lighting (Rizzutti’s sign), the Fleischer ring, “iron deposits” within the epithelium near the cone base, and finally the presence of Vogt’s striae.17 The KCN diagnosis was further confirmed by the WaveLight® Oculyzer™ II (Alcon Surgical, Fort Worth, TX, USA) and a Pentacam High-Resolution Scheimpflug imaging camera (Oculus Optikgeräte, Wetzlar, Germany).18 Quantitative data supporting the KCN diagnosis included keratometry >45.00 D and asymmetric topographic patterns. In addition to not having been subjected to any previous ocular intervention, for a patient to be included in study group A, the following criteria had to be met: absence of systemic disease, no history of chemical injury, or delayed epithelial healing.

The control group, B (n=160 different eyes), consisted of normal, healthy eyes, confirmed by a complete ophthalmologic evaluation and corneal imaging. While participants were randomly selected from the pool of screening patients, age and sex were considered in order to match study group A. Exclusion criteria for the control group (B) included current or past ocular pathology, previous surgery, present irritation, or dry-eye disorder, as well as contact lens wear.

Female patients with ongoing pregnancy or lactating at the time of the study were excluded from either group. In all cases, to minimize testing variations, OCT and Scheimpflug imaging were conducted by skilled trained investigators and preceded ocular clinical examination.

Sample-size determination

The sample size was established by the following parameters: estimated epithelial thickness variability (standard deviation), minimum spotted difference, significance criterion, and statistical power.19 The epithelial thickness variability was established by a recent study20 to be in the order of 2.5 μm. The minimum spotted difference was 1 μm, while the significance criterion was set to 0.05 and the intended statistical power was set to 0.95. Based on these criteria, the total sample size was estimated to be n=320.

OCT epithelial imaging

The Fourier-domain AS-OCT system RTVue® 100 (Optovue, Fremont, CA, USA), running on analysis and report software version A6 (9.0.27), was employed in the study. Data output included total corneal and epithelial thickness maps corresponding to a 6 mm-diameter area. The settings were: L-Cam lens, eight meridional B scans per acquisition, consisting of 1,024 A scans each with 5 μm axial resolution. The main analysis report produced by the system displayed total corneal (reported as pachymetry) and epithelial three-dimensional thickness maps covering the 6 mm-diameter area, examples of which are provided in Figure 1.

Figure 1 Total corneal (left) and epithelial (right) three-dimensional pachymetry maps. Top, a right eye with topographic variability of 8.4 μm and epithelial range of −28 mm, classified as KC2 (IHD =0.065, ISV =64); bottom, a right eye with topographic variability of 9.6 μm and epithelial range of −30 μm, classified as KC3 (IHD =0.107, ISV =104).
Notes: *Thickness minimum (in both corneal and epithelial maps); +thickness maximum (epithelial map only).
Abbreviations: IHD, index of height decentration; ISV, index of surface variance.

As shown in Figure 1, each pachymetry map is divided in to 17 sectors. Specifically, these are the 2 mm-diameter pupil-center disk of 12.56 mm2 area, eight sectors (octants) within the annulus between the 2–5 mm zones, each of 8.24 mm2 area, and eight sectors (octants) within the annulus of 5–6 mm zones, each of 4.32 mm2 area. For each one of these sectors, the average thickness is displayed numerically over the corresponding area.

In our study, the reported “epithelium center” thickness corresponded to the numeric indication over the center disk. The superior, inferior, minimum, and maximum thickness, as well as the topographic thickness variability (computed by the epithelial thickness standard deviation of the 17 segments) were obtained directly by the statistics report. Epithelial thickness range, defined as the minimum minus the maximum epithelial thickness, was derived accordingly. Mean epithelial thickness was computed by the integer values of the seventeen octant numerical results, and the peripheral epithelial thickness was computed by the average of the thickness corresponding to eighteen equispaced points along the 5 mm radius (data collected via mouse-over indication on the epithelial thickness map). To assess intraindividual repeatability, three consecutive acquisitions per eye were performed. The standard deviation of the three respective values was reported as repeatability, while the mean of these values has been included in the study.

Scheimpflug imaging anterior-surface topometric indices

Scheimpflug imaging was employed to provide simulated keratometry (in diopters), topographic KCN classification (TKC), and AC-irregularity indices.21 These data are offered, eg, by the “refractive” and “topometric” reports provided by the Pentacam software. Specifically, the TKC grading scheme comprised of the following stages, indicating not KCN, KC1, KC1–2, KC2, KC2–3, KC3, KC3–4, and KC4, indicating advancing stages of KCN, from the mildest to the most severe, respectively. To facilitate statistical analysis, we introduced a numeric conversion, ie, grade (−) was set to 0, KC1 to 1, KC1–2 to 2, KC2 to 3, KC2–3 to 4, KC3 to 5, KC3–4 to 6, and KC4 to 7, as was previously introduced.22

The AS-irregularity indices investigated in the study were: index of surface variance (ISV), the value of the standard deviation of individual corneal sagittal radii from the mean curvature; and index of height decentration (IHD), the value of the decentration of elevation data in the vertical direction, calculated from a Fourier analysis. Both of these indices have been recently studied in screening, classification, and clinical follow-up8,18,21,23 of keratoconic patients, indicating a robust correlation with KCN severity.

To investigate further the possible correlation of OCT-derived epithelial thickness characteristics with Scheimpflug-derived KCN severity, two subgroups were formed from group A. Specifically, subgroup A1 (n=40) was formed from the cases whose KCN grading (TKC) was less or equal to KC1, while subgroup A2 (n=58) was formed from the advanced KCN cases (TKC equal to or greater than KC3).

Descriptive statistics, linear regression, analysis of variance to seek possible correlations, and analysis of variance between subgroups were performed by Minitab version 16.2.3 (Minitab, Coventry, UK) and Origin® version 9 (OriginLab, Northampton, MA, USA). Paired analysis P-values less than 0.05 were considered an indication of statistically significant results. Results are presented in the form of means ± standard deviation of the mean (minimum to maximum).


The keratoconic study group (A) was formed from 160 eyes, of which 72 belonged to female and 88 belonged to male patients. The mean age of patients at the time of examination was 33.8±9.7 (18–60) years. There were 83 right (oculus dexter) and 77 left (oculus sinister) eyes. Average simulated keratometry for group A (KCN), as provided by the Scheimpflug imaging, was K1 (flat) 46.63±3.78 D, and K2 (steep) 50.86±5.14 D. Average TKC was between the KC2 and the KC2–3 stages. The AS-irregularity indices had the following values: IHD 0.076±0.055 (0.030–0.287) and ISV 82.8±47.7 (10–237).

The control group (B) was formed from 160 eyes, of which 70 belonged to female and 90 to male patients. The mean age of patients at the time of examination was 34.8±9.3 (18–59) years. There were 80 right and 80 left eyes. Average simulated keratometry for this control group, as provided by the Scheimpflug imaging, was K1 (flat) 42.78±1.33 D and K2 43.47±1.95 D. Average TKC was (−). The AS-irregularity indices had the following values: IHD 0.029±0.21 and ISV 31.78±22.61.

Repeatability and ease of OCT measurement

In all cases, following correct fixation and centering, OCT epithelial imaging-acquisition time was less than 0.5 second. No patient reported reservations or expressed discomfort regarding this noncontact measurement. Following acquisition, the epithelial thickness summary report was furnished in less than 1 minute.

Intraindividual repeatability for the epithelial thickness for the center, superior, inferior, maximum, minimum, and topographic thickness variability was evaluated by the standard deviation of four consecutive acquisitions in each case. As reported in Table 1, for study group A (keratoconic eyes), the epithelial thickness had an average repeatability for center 1.78±1.31 μm, superior 1.92±0.99 μm, inferior 1.58±1.01 μm, minimum 1.33±1.15 μm, maximum 1.72±1.30 μm, and topographic thickness variability 0.46±0.25 μm. For the control group (B, normal eyes), the epithelial thickness had an average repeatability for center 0.89±0.75 μm, superior 1.21±0.89 μm, inferior 0.73±0.67 μm, minimum 1.07±1.02 μm, maximum 1.73±1.05 μm, and topographic thickness variability 0.19±0.11 μm, respectively.

Table 1 Intraindividual-repeatability descriptive statistics for epithelial thickness, as computed by the standard deviation of the respective values obtained by four consecutive acquisitions per eye
Notes: Repeatability is reported for the epithelium at the pupil center (center), superior, inferior, as well as minimum, maximum, and topographic thickness variability. All values are in micrometers.
Abbreviations: KCN, keratoconus; SD, standard deviation.

Epithelial thickness statistical analysis

For study group A (keratoconic eyes), the center epithelial thickness was on average 51.75±7.02 (36–72) μm, with superior 55.57±6.79 (42–76) μm, inferior 49.65±5.54 (33–72) μm, minimum 40.73±8.51 (19–60) μm, maximum 63.54±8.85 (49–94) μm, mean 52.39±5.48 (43.50–70.33) μm, and mid-peripheral thickness 52.52±5.36 (43.60 –70.00) μm. For the control group (B, healthy eyes), the center epithelial thickness was 52.54±3.23 (45–59) μm, with superior 51.35±3.41 (44–60) μm, inferior 53.13±3.27 (45–59) μm, minimum 48.50±3.98 (28–57) μm, maximum 55.33±3.67 (46–64) μm, mean 52.24±3.21 (44.58–59.42) μm, and mid-peripheral thickness 52.18±3.23 (44.50–59.50) μm. These results are reported in Table 2 and are illustrated in the form of box plots in Figure 2.

Table 2 Central epithelial thickness (center), superior, inferior, minimum, maximum, topographic thickness variability, range, mean, and peripheral for the two study groups (control and keratoconus [KCN])
Notes: Subgroup A1 was defined by keratoconic patients classified by topographic KCN classification (TKC) less than or equal to 1, and subgroup A2 defined by TKC equal to or greater than 3. All values are in micrometers.

Figure 2 Box plots of epithelial thickness (showing center, superior, inferior, minimum, maximum, mean, and peripheral) showing median level (⊗), average (⊕), 95% median confidence, and interquartile interval range boxes. Top, keratoconic group; bottom, control group.

The epithelium was thinner in the center in the KCN group (−0.79 μm), while thicker overall (+0.15 μm) and mid-peripherally (+0.35 μm) in comparison to the control group (0.0760 and 0.0491, respectively; P=0.0199). The epithelium at the superior position (+4.22 μm) and maximum (+8.21 μm) was thicker, while the inferior (−3.48 μm) and minimum (−7.77 μm) were thinner in the KCN group in comparison to the control group (in all cases, P<0.01). More pronounced were the differences in topographic thickness variability (+4.48 μm) and range (−15.94 μm), both significant statistical differences (P<0.001). Figure 3 illustrates (in the form of box plots) epithelial thickness topographic variability and epithelial thickness range for the two groups. We note the negative sign in the thickness range, a result of the definition adopted by the AS-OCT software, which we preserved in this study for conformity.

Figure 3 Box plots of epithelial thickness topographic variability (top) and range, defined as minimum minus maximum (bottom) for the two groups. Graphs include median level (⊗), average (⊕), 95% median confidence, and interquartile interval range boxes.
Abbreviation: KCN, keratoconus.

Epithelial thickness in relation to keratoconus stage

We further investigated distribution characteristics within the two subgroups formed within study group A, namely the lower keratoconic stage A1 and the advanced-stage subgroup A2. The center epithelium was thicker in the lower keratoconic stage subgroup A1 (+0.36 μm, P=0.118) and thinner in the advanced-stage subgroup A2 (−1.93 μm, P=0.032) in comparison to the control group. Mean and peripheral epithelial thickness was also higher in the lower keratoconic subgroup A1 (+0.63 and +1.14 μm, P=0.045 and 0.021, respectively) in comparison to the control group. Detailed descriptive statistics are reported in Table 2.

The epithelial thickness topographic variability was 2.46±0.75 (−4 to −18) μm in the lower keratoconic stage subgroup A1 and 9.27±3.25 (−14 to −66) μm in the advanced-stage subgroup A2. The analysis between topographic variability for the control group (B), KCN group (A), and subgroups A1 and A2 indicated (P<0.01) that the means differed at the 0.05 level of significance. Similar results were obtained for the epithelial range: in the advanced KCN subgroup A2, the epithelial thickness range was −34.05±11.31 (−14 to −66) μm, in the entire KCN group −22.81±12.55 (−4 to −66) μm, and in the low KCN subgroup A1 −10.33±3.82 (−4 to −18) μm, while in the control group it was −6.86±3.33 (−3 to −29) μm (P<0.001).

Epithelial thickness topographic variability and range compared to Scheimpflug-derived anterior-surface irregularity indices

As indicated by the statistically significant differences observed in the topographic thickness variability and thickness range (difference of the minimum minus the maximum) in relation to the keratoconic severity, we further considered the one-to-one correlation of these two epithelial thickness topographic variables, obtained by OCT with two AS-irregularity indices, IHD and ISV, obtained by Scheimpflug imaging. Specifically, regression analysis was performed on the following pairs: epithelial thickness variability versus IHD and ISV, and epithelial thickness range versus IHD and ISV. Regression analysis indicated statistically significant relationships (P<0.01 in both pairs; coefficients of determination 0.58, 0.61, 0.53, and 0.58, respectively). Scatter and fitted-line plots for these relationships are illustrated in Figures 4 and 5.

Figure 4 Scatter and fitted-line plots of topographic epithelial thickness variability versus index of height decentration (IHD; top) and index of surface variance (ISV; bottom). Graphs include regression, 95% confidence interval (CI), and 95% prediction interval (PI) lines.

Figure 5 Scatter and fitted-line plots of topographic epithelial thickness range versus index of height decentration (IHD; top) and index of surface variance (ISV; bottom). Graphs include regression, 95% confidence interval (CI), and 95% prediction interval (PI) lines.


Clinical in vivo epithelial mapping by AS-OCT is currently in practice, and introduces a simple and effective clinical tool for corneal epithelium mapping. The data are very easily obtained, and epithelial thickness parameters are automatically calculated by the system software report.20,24 The ease of use, as well as the reliable and predictable measurement, as indicated by the low intraindividual repeatability,25 which was only slightly elevated in the keratoconic group (Table 1), suggest that epithelial imaging by AS-OCT holds promise for wider clinical application, such as screening of young adults for early KCN, and in a much wider perspective, potential candidates for laser cornea refractive surgery.

One may wonder what the clinical potential of epithelial thickness measurements might be and what this technology could accomplish that other imaging technologies in widespread use might not? One possibility lies in the fact that irregular epithelial thickness distribution can “compensate” for underlying irregular stromal distribution,26,27 and thus may mask topographic and tomographic results, particularly in early stage KCN investigation. We believe therefore that epithelial thickness imaging may be extremely helpful in KCN investigation by revealing the accentuated stromal irregularities that may not be detected by traditional corneal topography.

The comprehensive study presented herein not only confirms our previous findings10,13 regarding increased overall epithelial thickness and variation in keratoconic eyes, and to a much lesser degree in less affected eyes, but also that the newly clinically available AS-OCT feature of in vivo epithelial imaging may illustrate these differences in a more reliable, repeatable, and quantitative way.

The epithelium in the keratoconic eye group presents with relatively increased overall levels and noticeably increased thickness variations (in simple terms, not only choppy waters, but high tide as well). Specifically, the defining qualitative feature of the epithelium in the keratoconic group was the pattern of thin epithelium over the cone (corresponding to the thinnest cornea, Figure 1), typically in the temporal/inferior area, while a thicker epithelium appears in the superior/nasal area, away from the cone. It is noteworthy that in the normal population (group B in this study), and also extensively presented in Kanellopoulos and Asimellis,20 the superior epithelium was thinner superiorly than inferiorly; the opposite held true in the keratoconic group (Table 2, Figure 2). These thickness irregularities are pronounced with increasing severity of KCN.

Quantitatively, these differences can be expressed by the epithelial minimum being on average −7.77 μm and inferior −3.48 μm thinner in the keratoconic group, while maximum and superior thickness were thicker by +8.21 μm and +4.22 μm in the keratoconic group, in comparison to the controls. This pattern is in agreement with previous results obtained with high-frequency ultrasound biomicroscopy1,10 and OCT.13,15

Overall epithelial thickness variability as well as thickness range appear even more significant. For example, in the keratoconic group, thickness variability (+4.48 μm, or 380% more) and range (−15.94 μm, or 332% more) increased a large margin and were statistically significant compared to the control group (Table 2), in agreement with a previous study.2 More importantly, these indices, derived by clinical OCT imaging, were well correlated with established KCN indices, such as the Pentacam-derived topographic AS-irregularity indices of ISV and IHD (Figures 4 and 5). Such a comparison has not been previously reported in the peer-reviewed literature in kind or in scale, and in our opinion presents a novel, specific, and sensitive tool for the clinician.

Although not documented yet, epithelium depiction with AS-OCT probably represents longer-term remodeling associated with KCN, and not a temporary effect due to recent eye rubbing. It could be the irregular corneal shape and thickness (although normalization of the epithelium following corneal cross-linking treatment minimizes the influence of this theory), as has been introduced by our team.7 It could be related to eye rubbing, although regular stability of the epithelial maps through long-term investigation also minimized the potential influence of this theory. Finally, it appears that the specific epithelial pattern that is encountered in keratoconic eyes is directly related to the degree of biomechanical instability, and thus it may be linked to partial deformation of the cornea associated with blinking and eye rubbing, but more importantly we feel that normal oscillation of the cornea due to lateral and vertical oscillation of the eye and its possible inertia effect on the corneal biomechanics, during normal binocular fixation, reaching the astounding speeds of 900 degrees/sec.28,29 Additionally possibly due to the fluid pulse wave reaching the cornea posteriorly through aqueous conduction and deriving from the arterial ventricular human heart pulse wave.10 Partial “normalization” of the epithelial pattern following collagen cross-linking further supports this theory. The comprehensive investigation of epithelial thickness by AS-OCT in this work suggests high predictability of measurement in KCN patients. Our previously reported finding of overall epithelial thickness in keratoconic eyes in comparison to normal when studied with high frequency ultrasound, has been confirmed with this technology as well. Of particular diagnostic interest is the relation of this finding to lower/earlier stages of keratoconus or even preclinical keratoconus manifestation. The increase in topographic thickness variability and range, easily captured by the device studied, has been identified to be in very close correlation with severity of keratoconus. Additionally the OCT-derived epithelial topographic thickness variability and epithelial thickness range correlate remarkably with established Scheimpflug imaging-derived anterior surface-irregularity indices for keratoconus. Again of particular clinical significance, the interest would be this relation in lower/earlier keratoconus stages, or even when evaluating the fellow “healthy” unaffected cornea of a keratconus patient, especially of younger age.


Corneal collagen crosslinking has changed the therapeutic paradigm for keratoconus and ectasia, dictating vigorous work on the side of the clinician on early diagnosis and more sensitive progression detection. As cornea imaging modalities multiply, early detection and sensitive progression monitoring appears to redefine the traditional metrics used up until now. Epithelial imaging has been probably greatly ignored up until recently, due to mainly the technical difficulty of its imaging capture, may become an easy and important clinical tool with the help of OCT.

Author contributions

Design of the study (AJK); data collection (AJK), management (AJK), analysis (AJK, GA), interpretation of the data (AJK, GA); manuscript preparation (AJK, GA), manuscript critical revision (AJK, GA), manuscript approval (AJK).


AJK holds consultant/advisory positions at Alcon/WaveLight, Avedro, i-Optics, Allergan and Keramed. GA reports no conflicts of interest in this work.



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