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Multifaceted Characterization And In Vitro Assessment Of Polyurethane-Based Electrospun Fibrous Composite For Bone Tissue Engineering

Authors Jiang H, Mani MP, Jaganathan SK

Received 6 May 2019

Accepted for publication 28 August 2019

Published 8 October 2019 Volume 2019:14 Pages 8149—8159

DOI https://doi.org/10.2147/IJN.S214646

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Melinda Thomas

Peer reviewer comments 2

Editor who approved publication: Prof. Dr. Anderson Oliveira Lobo


Haoli Jiang,1 Mohan Prasath Mani,2 Saravana Kumar Jaganathan3–6

1Orthopaedics Department, The Third People’s Hospital of Shenzhen, Shenzhen, Guangdong 518114, People’s Republic of China; 2School of Biomedical Engineering and Health Sciences, Faculty of Engineering, Universiti Teknologi Malaysia, Skudai 81310, Malaysia; 3Department for Management of Science and Technology Development, Ton Duc Thang University, Ho Chi Minh City, Vietnam; 4Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, Vietnam; 5IJNUTM Cardiovascular Engineering Center, School of Biomedical Engineering and Health Sciences, Faculty of Engineering, Universiti Teknologi Malaysia, Skudai 81310, Malaysia; 6Department of Engineering, Faculty of Science and Engineering, University of Hull, Hull HU6 7RX, UK

Correspondence: Saravana Kumar Jaganathan
Department for Management of Science and Technology Development, Ton Duc Thang University, Ho Chi Minh City, Vietnam
Tel +84 28837755037
Email saravana@tdtu.edu.vn

Introduction: Recently several new approaches were emerging in bone tissue engineering to develop a substitute for remodelling the damaged tissue. In order to resemble the native extracellular matrix (ECM) of the human tissue, the bone scaffolds must possess necessary requirements like large surface area, interconnected pores and sufficient mechanical strength.
Materials and methods: A novel bone scaffold has been developed using polyurethane (PE) added with wintergreen (WG) and titanium dioxide (TiO2). The developed nanocomposites were characterized through field emission scanning electron microscopy (FESEM), Fourier transform and infrared spectroscopy (FTIR), X-ray diffraction (XRD), contact angle measurement, thermogravimetric analysis (TGA), atomic force microscopy (AFM) and tensile testing. Furthermore, anticoagulant assays, cell viability analysis and calcium deposition were used to investigate the biological properties of the prepared hybrid nanocomposites.
Results: FESEM depicted the reduced fibre diameter for the electrospun PE/WG and PE/WG/TiO2 than the pristine PE. The addition of WG and TiO2 resulted in the alteration in peak intensity of PE as revealed in the FTIR. Wettability measurements showed the PE/WG showed decreased wettability and the PE/WG/TiO2 exhibited improved wettability than the pristine PE. TGA measurements showed the improved thermal behaviour for the PE with the addition of WG and TiO2. Surface analysis indicated that the composite has a smoother surface rather than the pristine PE. Further, the incorporation of WG and TiO2 improved the anticoagulant nature of the pristine PE. In vitro cytotoxicity assay has been performed using fibroblast cells which revealed that the electrospun composites showed good cell attachment and proliferation after 5 days. Moreover, the bone apatite formation study revealed the enhanced deposition of calcium content in the fabricated composites than the pristine PE.
Conclusion: Fabricated nanocomposites rendered improved physico-chemical properties, biocompatibility and calcium deposition which are conducive for bone tissue engineering.

Keywords: polymer, TiO2/wintergreen, surface properties, apatite formation, tissue engineering

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