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Long Noncoding RNA LINC00173 Promotes NUTF2 Expression Through Sponging miR-765 and Facilitates Tumorigenesis in Glioma

Authors Du Q, Liu J, Tian D, Zhang X, Zhu J, Qiu W, Wu J

Received 12 May 2020

Accepted for publication 23 July 2020

Published 12 August 2020 Volume 2020:12 Pages 7211—7217

DOI https://doi.org/10.2147/CMAR.S262279

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Sanjeev Srivastava


Qinghua Du,1,* Jin Liu,1,* Da Tian,1 Xuelei Zhang,1 Jinwei Zhu,1 Weiwen Qiu,2 Jun Wu3

1Neurosurgery Department, Lishui City People’s Hospital, Lishui 323000, People’s Republic of China; 2Neurology Department, Lishui City People’s Hospital, Lishui 323000, People’s Republic of China; 3Pathology Department, Lishui City People’s Hospital, Lishui 323000, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Jun Wu; Weiwen Qiu Email wwwwjjjj924@163.com; weiwenq@hotmail.com

Background: Glioma is one of the leading causes of cancer-related deaths. This study aimed to investigate the function and mechanism of long noncoding RNA (lncRNA) LINC00173 in the regulation of glioma progression.
Methods: LINC00173 expression was measured using qRT-PCR. Survival rate was analyzed through Kaplan–Meier method. CCK8, colony formation and EdU assays were performed to measure cell proliferation while transwell was used to determine cell migration and invasion. Luciferase reporter assay was conducted to test RNA interaction.
Results: LINC00173 expression was elevated in glioma tissues and cells. LINC00173 high expression predicted poor prognosis. Loss of LINC00173 inhibited proliferation, migration and invasion. LINC00173 interacted with miR-765 to enhance NUTF2 expression. MiR-765 expression was negatively correlated with LINC00173 and NUTF2 in glioma tissues. NUTF2 level was increased in glioma tissues. NUTF2 overexpression rescued the potential of proliferation, migration and invasion in LINC00173-silenced cells.
Conclusion: Our research demonstrated that LINC00173 promotes glioma progression through targeting miR-765/NUTF2 axis.

Keywords: LINC00173, miR-765, NUTF2, glioma

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